본문 바로가기
KERD
논문KCI 등재

Tat-ATOX1 inhibits inflammatory responses via regulation of MAPK and NF-κB pathways

1

조회

0

다운로드

6

인용

0

참고문헌

키워드

주제분류화학

초록

Antioxidant 1 (ATOX1) protein has been reported to exhibit various protective functions, including antioxidant and chaperone. However, the effects of ATOX1 on the inflammatory response has not been fully elucidated. Thus, we prepared cell permeable Tat-ATOX1 and studied the effects on lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13- acetate (TPA)-induced inflammation. Experimental results showed that transduced Tat-ATOX1 protein significantly suppressed LPS-induced intracellular reactive oxygen species (ROS). Also, Tat-ATOX1 protein markedly inhibited LPS- and TPA-induced inflammatory responses by decreasing cyclooxygenase- 2 (COX-2) and inducible nitric oxide synthase (iNOS) and further inhibited phosphorylation of mitogen activated protein kinases (MAPKs; JNK, ERK and p38) and the nuclear factor-kappaB (NF-κB) signaling pathway. These results indicate that the Tat-ATOX1 protein has a pivotal role in inflammation via inhibition of inflammatory responses, suggesting Tat-ATOX1 protein may offer a therapeutic strategy for inflammation.

Abstract

Antioxidant 1 (ATOX1) protein has been reported to exhibit various protective functions, including antioxidant and chaperone. However, the effects of ATOX1 on the inflammatory response has not been fully elucidated. Thus, we prepared cell permeable Tat-ATOX1 and studied the effects on lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13- acetate (TPA)-induced inflammation. Experimental results showed that transduced Tat-ATOX1 protein significantly suppressed LPS-induced intracellular reactive oxygen species (ROS). Also, Tat-ATOX1 protein markedly inhibited LPS- and TPA-induced inflammatory responses by decreasing cyclooxygenase- 2 (COX-2) and inducible nitric oxide synthase (iNOS) and further inhibited phosphorylation of mitogen activated protein kinases (MAPKs; JNK, ERK and p38) and the nuclear factor-kappaB (NF-κB) signaling pathway. These results indicate that the Tat-ATOX1 protein has a pivotal role in inflammation via inhibition of inflammatory responses, suggesting Tat-ATOX1 protein may offer a therapeutic strategy for inflammation.

참고문헌 (42)

  1. Martinon F, "The inflammasomes:guardians of the body", Annu Rev Immunol, 27, pp.229~265 (2009)
  2. Medzhitov R, "Origin and physiological roles of inflammation", Nature, 454, pp.428~435 (2008)
  3. Hiraiwa K, "Contribution of lung macrophages to the inflammatory responses induced by exposure to air pollutants", Mediators Inflamm, 2013, pp.619523~ (2013)
  4. Fujiwara N, "Macrophages in inflammation", Curr Drug Targets Inflamm Allergy, 4, pp.281~286 (2005)
  5. Laskin DL, "Macrophages and tissue injury: agents of defense or destruction", Annu Rev Pharmacol Toxicol, 51, pp.267~288 (2011)
  6. Haque MA, "Zerumbone suppresses the activation of inflammatory mediators in LPS-stimulated U937 macrophages through MyD88-dependent NF-B", MAPK, Int Immunopharmacol, pp.2018, 55[DOI]
  7. Aggarwal BB, "Nuclear factor-kappaB: the enemy within", Cancer Cell, 6, pp.203~208 (2004)
  8. Yang Y, "Functional roles of p38mitogen activated protein kinase in macrophage-mediated inflammatory responses", Mediators Inflamm, 2014, pp.352371~ (2014)
  9. Hwang PA, "Inhibition of lipopolysaccharide (LPS)-induced inflammatory responses by Sargassum hemiphyllum sulfated polysaccharide extract in RAW 264.7 macrophage cells", J Agric Food Chem, 59, pp.2062~2068 (2011)
  10. Hung IH, "HAH1 is a copper-binding protein with distinct amino acid residues mediating copper homeostasis and antioxidant defense", J Biol Chem, 273, pp.1749~1754 (1998)
  11. Montes S, "Copper and copper proteins in Parkinson's disease", Oxid Med Cell Longev, 147251, pp.2014~ (2014)
  12. Kelner GS, "The copper transport protein Atox1 promotes neuronal survival", J Biol Chem, 275, pp.580~584 (2000)
  13. Ahn EH, "Tat-ATOX1inhibits streptozotocin-induced cell death in pancreatic RINm5F cells and attenuates diabetes in a mouse model", Int J Mol Med, 38, pp.217~224 (2016)
  14. Chen GF, "Copper transport protein antioxidant-1 promotes inflammatory neovascularization via chaperone and transcription factor function", Sci Rep, 5, pp.14780~ (2015)
  15. Hasselbalch HC, "Whole blood transcriptional profiling reveals deregulation of oxidative and antioxidative defence genes in myelofibrosis and related neoplasms. Potential implications of downregulation of Nrf2 for genomic instability and disease progression", PLoS One, 9, pp.e112786~ (2014)
  16. Hatori Y, "Functional partnership of the copper export machinery and glutathione balance in human cells", J Biol Chem, 287, pp.26678~26687 (2012)
  17. Kelner GS, "The copper transport protein Atox1 promotes neuronal survival", J Biol Chem, 275, pp.580~584 (2000)
  18. Ozumi K, "Role of copper transport protein antioxidant 1 in angiotensin II-induced hypertension: a key regulator of extracellular superoxide dismutase", Hypertension, 60, pp.476~486 (2012)
  19. Dolgova NV, "Copper chaperone Atox1 interacts with the metal-binding domain of Wilson's disease protein in cisplatin detoxification", Biochem J, 454, pp.147~156 (2013)
  20. Wadia JS, "Protein transduction technology", Curr Opin Biotechnol, 13, pp.52~56 (2002)
  21. van den Berg A, "Protein transduction domain delivery of therapeutic macromolecules", Curr Opin Biotechnol, 22, pp.888~893 (2011)
  22. Kubo E, "TAT-mediated PRDX6 protein transduction protects against eye lens epithelial cell death and delays lens opacity", Am J Physiol Cell Physiol, 294, pp.C842~C855 (2008)
  23. Embury J, "Proteins linked to a protein transduction domain efficiently transduce pancreatic islets", Diabetes, 50, pp.1706~1713 (2001)
  24. Kim SM, "Tat-antioxidant 1protects against stress-induced hippocampal HT-22 cells death and attenuate ischemic insult in animal model", J Cell Mol Med, 19, pp.1333~1345 (2015)
  25. 여현지, "Protective effects of Tat-DJ-1 protein against streptozotocin-induced diabetes in a mice model", BMB Reports, 51(7), pp.362~367 (2018), 생화학분자생물학회
  26. 조효상, "Effects of PEP-1-FK506BP on cyst formation in polycystic kidney disease", BMB Reports, 50(9), pp.460~465 (2017), 생화학분자생물학회
  27. Jo HS, "Tat-HSP27 inhibits oxidative stress-induced hippocampal neuronal cell death by regulation of the mitochondrial pathway", Mol Brain, 10(1) (2017)
  28. Shi Q, "Geniposde suppresses LPS-induced nitric oxide, PGE2 and inflammatory cytokine by downregulating NF-kappaB, MAPK and AP-1signaling pathways in macrophages", Int Immunopharmacol, 20, pp.298~306 (2014)
  29. Lee HJ, "Chikusetsusaponin IVa methyl ester isolated from the roots of achyranthes japonica suppresses LPS-induced iNOS, TNF-alpha, IL-6, and IL-1beta expression by NF-kappaB and AP-1inactivation", Biol Pharm Bull, 39, pp.657~664 (2016)
  30. Yodkeeree S, "O-methybulbocapnine and dicentrine suppress LPS-induced inflammatory responses by blocking NF-B and AP-1 inactivation through inhibition MAPKs and Akt signaling in RAW264.7 macrophages", Biol Pharm Bull, 41, pp.1219~1227 (2018)
  31. Qiang Z, "Inhibitory effect of different Dendrobium species on LPS-induced inflammation in macrophages via suppression of MAPK pathways", Chin J Nat Med, 16, pp.481~489 (2018)
  32. Harikrishnan H, "Anti-inflammatory effect of phyllanthus amarus Schum. & Thonn. Through inhibition of NF-B, MAPK, and PI3K-Akt signaling pathways in LPS-induced human macrophages", BMC Complement Altern Med, 18, pp.224~ (2018)
  33. Islam SU, "Decursinol angelate inhibits LPS-induced macrophages polarization through modulation of the NF-B and MAPK signaling pathways", Molecules, 23, pp.1880~ (2018)
  34. Stanley PL, "Mouse skin inflammation induced by multiple topical applications of 12-O-tetradecanoyl phorbol-13-acetate", Skin Pharmacol, 4, pp.262~271 (1991)
  35. Kim MJ, "PEP-1-PON1protein regulates inflammatory response in raw 264. 7macrophages and ameliorates inflammation in a TPAinduced animal model", PLoS One, 9, pp.e86034~ (2014)
  36. Hsu CH, "Hexahydro--acids potently inhibit 12-O-tetradecanoylphorbol 13-acetateinduced skin inflammation and tumor promotion in mice", J Agric Food Chem, 61, pp.11541~11549 (2013)
  37. Nagaraj M. Kulkarni, "Topical atorvastatin ameliorates 12-O-tetradecanoylphorbol-13- acetate induced skin inflammation by reducing cutaneous cytokine levels and NF-jB activation", Archives of Pharmacal Research, 38(6), pp.1238~1247 (2015), 대한약학회
  38. Kamiya T, "Copper chaperone antioxidant-1, Atox-1, is involved in the induction of SOD3 in THP-1 cells", Biometals, 31, pp.61~68 (2018)
  39. Ha HY, "Inhibition of the TPA-induced cutaneous inflammation and hyperplasia by EC-SOD", Biochem Biophys Res Commun, 348, pp.450~458 (2006)
  40. Kim SH, "Overexpression of extracellular superoxide dismutase(EC-SOD)in mouse skin plays a protective role in DMBA", TPA-induced tumor formation, 2005, pp.15, 333~341
  41. Bradford M, "A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein-dye binding", Anal Biochem, 72, pp.248~254 (1976)
  42. 양승주, "Anti-inflammatory and anti-oxidative effects of 3-(naphthalen-2-yl(propoxy)methyl)azetidine hydrochloride on β-amyloid-induced microglial activation", BMB Reports, 50(12), pp.634~639 (2017), 생화학분자생물학회

관련 논문AI추천

PEP-1-GLRX1 Protein Exhibits Anti-Inflammatory Effects by Inhibiting the Activation of MAPK and NF-κB Pathways in Raw 264.7 Cells박진서, 엄원식, 이성호, 최수영, 최연주, 한규형, Dae Won Kim, Hyun Ju Cha, Min Jea Shin, Sunghou Lee생화학분자생물학회 · BMB Reports2020 · 제53권 제2호Transduced Tat-Annexin protein suppresses inflammationassociated gene expression in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells강태천, 권오신, 김대원, 박은영, 박종훈, 박진서, 백수선, 음원식, 이선화, 조성우, 최수영, 한규형, 황현숙생화학분자생물학회 · BMB Reports2011 · 제44권 제7호Tat-GSTpi suppresses inflammatory responses by regulating ROS/MAPKs/apoptosis signaling pathwaysChan Hee Lee, Dae Won Kim, Duk-Soo Kim, Eun Ji Yeo, Gi Soo Youn, Hyeon Ji Yeo, Hyun Jung Kwon, Jong Kook Park, Jung Hwan Park, Keun Wook Lee, Lee Re Lee, Min Jea Shin, Na Yeon Kim, Soo Young Choi, Su Min Kim, Su Yeon Kwon, Won Sik Eum, Yeon Joo Choi생화학분자생물학회 · BMB Reports2025 · 제58권 제6호Transduced Tat-CIAPIN1 reduces the inflammatory response on LPS- and TPA-induced damages김대원, 김덕수, 김민영, 김정수, 신민재, 엄원식, 여현지, 유지호, 최수영생화학분자생물학회 · BMB Reports2019 · 제52권 제12호Suppression of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation in mice by transduced Tat-Annexin protein권혁일, 권형주, 김덕수, 박미영, 박진서, 음원식, 이선화, 최수영, 한규형, 황현숙, Dae Won Kim, Seon Ae Eom, Se-Young Jun생화학분자생물학회 · BMB Reports2012 · 제45권 제6호Tat-Thioredoxin-like protein 1 attenuates ischemic brain injury by regulation of MAPKs and apoptosis signaling김덕수, 최수영, Dae Won Kim, Eun Jeong Sohn, Eun Ji Yeo, Gi Soo Youn, Hyeon Ji Yeo, Hyun Ju Cha, Hyun Jung Kwon, Jung Hwan Park, Lee Re Lee, Min Jea Shin, Na Yeon Kim, Oh-Shin Kwon, Sung-Woo Cho, Su Yeon Kwon, Won Sik Eum, Yong-Jun Cho, Yu Ran Lee생화학분자생물학회 · BMB Reports2023 · 제56권 제4호

이 간행물의 다른 논문 (9건)