Imipramine enhances neuroprotective effect of PEP-1-Catalase against ischemic neuronal damage
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The protein transduction domains have been reported to have potential to deliver the exogenous molecules, including proteins,to living cells. However, poor transduction of proteins limits therapeutic application. In this study, we examined whether imipramine could stimulate the transduction efficiency of PEP-1 fused proteins into astrocytes. PEP-1-catalase (PEP-1-CAT) was transduced into astrocytes in a time- and dose-dependent manner, reducing cellular toxicity induced by H2O2. Additionally, the group of PEP-1-CAT + imipramine showed enhancement of transduction efficiency and therefore increased cellular viability than that of PEP-1-CAT alone. In the gerbil ischemia models, PEP-1-CAT displayed significant neuroprotection in the CA1 region of the hippocampus. Interestingly,PEP-1-CAT + imipramine prevented neuronal cell death and lipid peroxidation more markedly than PEP-1-CAT alone. Therefore, our results suggest that imipramine can be used as a drug to enhance the transduction of PEP-1 fusion proteins to cells or animals and their efficacies against various disorders. [BMB reports 2011; 44(10): 647-652]
Abstract
The protein transduction domains have been reported to have potential to deliver the exogenous molecules, including proteins,to living cells. However, poor transduction of proteins limits therapeutic application. In this study, we examined whether imipramine could stimulate the transduction efficiency of PEP-1 fused proteins into astrocytes. PEP-1-catalase (PEP-1-CAT) was transduced into astrocytes in a time- and dose-dependent manner, reducing cellular toxicity induced by H2O2. Additionally, the group of PEP-1-CAT + imipramine showed enhancement of transduction efficiency and therefore increased cellular viability than that of PEP-1-CAT alone. In the gerbil ischemia models, PEP-1-CAT displayed significant neuroprotection in the CA1 region of the hippocampus. Interestingly,PEP-1-CAT + imipramine prevented neuronal cell death and lipid peroxidation more markedly than PEP-1-CAT alone. Therefore, our results suggest that imipramine can be used as a drug to enhance the transduction of PEP-1 fusion proteins to cells or animals and their efficacies against various disorders. [BMB reports 2011; 44(10): 647-652]
참고문헌 (29)
- Koenitzer, J. R, "Redox signaling in", Ann. N. Y. Acad. Sci, 1203, pp.45~52 (2010)
- Bickers, D. R, "Oxidative stress in the pathogenesis of skin disease", J. Invest. Dermatol, 126, pp.2565~2575 (2006)
- Mates, J. M, "Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology", Toxicol, 83, pp.83~104 (2000)
- Muzykantov, V. R, "Targeting of superoxide dismutase and catalase to vascular endothelium", J. Control. Release, 71, pp.1~21 (2001)
- Kim, D. W, "Transduced human PEP-1-catalase fusion protein attenuates ischemic neuronal damage", FREE RADICAL BIOLOGY AND MEDICINE, 47, pp.941~952 (2009), Elsevier
- Eum, W. S, "HIV-1 Tat-mediated protein transduction of Cu,Zn-superoxide dismutase into pancreatic β cells in vitro and in vivo", Free Radical Biology and Medicine, 37(3), pp.339~349 (2004), Elsevier BV
- Ahn, E. H, "Transduced PEP-1-ribosomal protein S3 (rpS3) ameliorates 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice", TOXICOLOGY, 276, pp.192~197 (2010), ELSEVIER IRELAND LTD
- Egleton, R. D, "Bioavailability and transport of peptides and peptide drugs into the brain", Peptides, 18, pp.1431~1439 (1997)
- Behlke, M. A, "Chemical modification of siRNAs for in vivo use", Oligonucleotides, 18, pp.305~320 (2008)
- Dietz, G. P, "Cell-penetrating peptide technology to deliver chaperones and associated factors in diseases and basic research", Curr. Pharm. Biotechol, 11, pp.167~174 (2010)
- Matsui, H, "Protein therapy: in vivo protein transduction by polyarginine (11R) PTD and subcellular targeting delivery", Curr. Protein Pept. Sci, 4, pp.151~157 (2003)
- Prochiantz, J, "Messenger proteins: homeoproteins, TAT and others", Curr. Opin. Cell Biol, 12, pp.400~406 (2000)
- Schwarze, S. R, "In vivo protein transduction: delivery of a biologically active protein into the mouse", Science, 285, pp.1569~1572 (1999)
- Schwarze, S. R, "Protein transduction: unrestricted delivery into all cells", Trends Cell Biol, 10, pp.290~295 (2000)
- Wadia, J. S, "Protein transduction technology", Curr. Opin. Biotechnol, 13, pp.52~56 (2002)
- Wadia, J. S, "Modulation of cellular function by TAT mediated transduction of full length proteins", Curr. Protein Pept. Sci, 4, pp.97~104 (2003)
- Peng, C. H, "Neuroprotection by Imipramine against lipopolysaccharide-induced apoptosis in hippocampusderived neural stem cells mediated by activation of BDNF and the MAPK pathway", Eur. Neuropsychopharmacol, 18, pp.128~140 (2008)
- Keilhoff, G, "Cell proliferation is influenced by bulbectomy and normalized by imipramine treatment in a region-specific manner", Neuropsychopharmacol, 31, pp.1165~1176 (2006)
- Schiavon, A. P, "Imipramine enhances cell proliferation and decreases neurodegeneration in the hippocampus after transient global cerebral ischemia in rats", Neurosci. Lett, 470, pp.43~48 (2010)
- Abdel-Salam, I. M, "Evaluation of the anti-inflammatory and anti-nociceptive effects of different antidepressants in the rat", Pharmacol. Res, 48, pp.157~165 (2003)
- Hashioka, S, "Antidepressants inhibit interferon-gamma-induced microglial production of IL-6 and nitric oxide", Exp. Neurol, 206, pp.33~42 (2007)
- Hwang, J, "Inhibition of glial inflammatory activation and neurotoxicity by tricyclic antidepressants", NEUROPHARMACOLOGY, 55, pp.826~834 (2008), PERGAMON-ELSEVIER SCIENCE LTD
- Wang, H, "Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide", J. Control. Rel, 143, pp.64~70 (2010)
- Sun Hwa Lee, "Enhancement of HIV-1 Tat fusion protein transduction efficiency by bog blueberry anthocyanins", BMB reports, 43(8), pp.561~566 (2010), 생화학분자생물학회
- Zhang, D. L, "Oral administration of Crataegus flavonoids protects against ischemia", reperfusion brain damage in gerbils, 2004, pp.90, 211~219
- Xia, Z, "Tricyclic antidepressants inhibit IL-6, IL-1 beta and TNF-alpha release in human blood monocytes and IL-2 and interferon- gamma in T cells", Immunopharmacol, 34, pp.27~37 (1996)
- Kuroda, Y, "Prediction of phospholipidosis- inducing potential of drugs by in vitro biochemical and physicochemical assays followed by multivariate analysis", Toxicol. In Vitro, 24, pp.661~668 (2010)
- Vistica, D. T, "Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production", Cancer Res, 51, pp.2515~2520 (1991)
- Ahang, D. L, "Oral administration of Crataegus flavonoids protects against ischemia", reperfusion brain damage in gerbils, 2004, pp.90, 211~219