본문 바로가기
KERD
논문KCI 등재

PEP-1-GSTpi protein enhanced hippocampal neuronal cell survival after oxidative damage

조수빈|조성우|신민재|김덕수|최수영|박진서|김대원|박정환|최연주|조용준|이치헌|권오신|조효상|여은지|음원식|손은정|손오라|Yeon Hee Yu
생화학분자생물학회 (Korean Society Of Biochemistry And Molecular Biology)|2016.07|BMB Reports|제49권 제7호|pp. 382-387

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

Oxidative stressIschemiaProtein therapyApoptotic signalsPEP-1- GSTpi
주제분류화학

초록

Reactive oxygen species generated under oxidative stress are involved in neuronal diseases, including ischemia. Glutathione S-transferase pi (GSTpi) is a member of the GST family and is known to play important roles in cell survival. We investigated the effect of GSTpi against oxidative stress-induced hippocampal HT-22 cell death, and its effects in an animal model of ischemic injury, using a cell-permeable PEP-1-GSTpi protein. PEP-1-GSTpi was transduced into HT-22 cells and significantly protected against H2O2-treated cell death by reducing the intracellular toxicity and regulating the signal pathways, including MAPK, Akt, Bax, and Bcl-2. PEP-1-GSTpi transduced into the hippocampus in animal brains, and markedly protected against neuronal cell death in an ischemic injury animal model. These results indicate that PEP-1-GSTpi acts as a regulator or an antioxidant to protect against oxidative stressinduced cell death. Our study suggests that PEP-1-GSTpi may have potential as a therapeutic agent for the treatment of ischemia and a variety of oxidative stress-related neuronal diseases.

Abstract

Reactive oxygen species generated under oxidative stress are involved in neuronal diseases, including ischemia. Glutathione S-transferase pi (GSTpi) is a member of the GST family and is known to play important roles in cell survival. We investigated the effect of GSTpi against oxidative stress-induced hippocampal HT-22 cell death, and its effects in an animal model of ischemic injury, using a cell-permeable PEP-1-GSTpi protein. PEP-1-GSTpi was transduced into HT-22 cells and significantly protected against H2O2-treated cell death by reducing the intracellular toxicity and regulating the signal pathways, including MAPK, Akt, Bax, and Bcl-2. PEP-1-GSTpi transduced into the hippocampus in animal brains, and markedly protected against neuronal cell death in an ischemic injury animal model. These results indicate that PEP-1-GSTpi acts as a regulator or an antioxidant to protect against oxidative stressinduced cell death. Our study suggests that PEP-1-GSTpi may have potential as a therapeutic agent for the treatment of ischemia and a variety of oxidative stress-related neuronal diseases.

참고문헌 (44)

  1. Udomsinprasert R, "Identification, characterization, structure of a new delta class glutathione transferase isoenzyme", Biochem J, 388, pp.763~771 (2005)
  2. Sheehan D, "Structure, function, evolution of glutathione transferases:implications for classification of non-mammalian members of an ancient enzyme superfamily", Biochem J, 36, pp.1~16 (2001)
  3. Raza H, "Dual localization of glutathione S-transferase in the cytosol, mitochondria: implications in oxidative stress, toxicity and disease", FEBS J, 278, pp.4243~4251 (2011)
  4. Atkinson HJ, "Glutathione transferases are structural, functional outliers in the thioredoxin fold", Biochemistry, 48, pp.11108~11116 (2009)
  5. Harrison DJ, "Distribution of glutathione S-transferase isoenzymes in human kidney: basis for possible markers of renal injury", J Clin Pathol, 42, pp.624~628 (1989)
  6. Landi S, "Mammalian class theta GST, differential susceptibility to carcinogens: a review", Mutat Res, 463, pp.247~283 (2000)
  7. Townsend DM, "The role of glutathione-S-transferase in anti-cancer drug resistance", Oncogene, 22, pp.7369~7375 (2003)
  8. Mari M, "Mitochondrial glutathione, a key survival antioxidant", Antioxid Redox Signal, 11, pp.2685~2700 (2009)
  9. Tew KD, "GST function in drug and stress response", Drug Resist Updat, 2, pp.143~147 (1999)
  10. Laborde E, "Glutathione transferases as mediators of signaling pathways involved in cell proliferation and cell death", Cell Death Differ, 17, pp.1373~1380 (2010)
  11. Thevenin AF, "GSTpi modulates JNK activity through a direct interaction with JNK substrate, ATF2", Protein Sci, 20, pp.834~848 (2011)
  12. Wu Y, "Human glutathione S-transferase P1-1 interacts with TRAF2, regulates TRAF2–ASK1 signals", Oncogene, 25, pp.5787~5800 (2006)
  13. Misra MK, "Oxidative stress, ischemic myocardial syndromes", Med Sci Monit, 15, pp.RA209~RA219 (2009)
  14. Halliwell B, "Oxidative stress, neurodegeneration:where are we now?", J Neurochem, 97, pp.1634~1658 (2006)
  15. Giorgio M, "Hydrogen peroxide: a metabolic by-product or a common mediator of ageing signals?", Nat RevMol Cell Biol, 8, pp.722~728 (2007)
  16. Coyle JT, "Oxidative stress, glutamate, and neurodegenerative disorders", Science, 262, pp.689~695 (1993)
  17. Kleikers PW, "NADPH oxidases as a source of oxidative stress, molecular target in ischemia", reperfusion injury, 2012, pp.90, 1391~1406
  18. Moro MA, "Mitochondrial respiratory chain and free radical generation in stroke", Free Radic Biol Med, 39, pp.1291~1304 (2005)
  19. Atlante A, "The apoptosis", necrosis transition in cerebellar granule cells depends on the mutual relationship of the antioxidant, the proteolytic systems which regulate ROS production and cytochrome c release enroute to death, 2003, pp.84, 960~971
  20. Jay SM, "Protein engineering for cardiovascular therapeutics: untapped potential for cardiac repair", Circ Res, 113, pp.933~943 (2013)
  21. Koren E, "Cell-penetrating peptides: breaking through to the other side", Trends Mol Med, 18, pp.385~393 (2012)
  22. Gump JM, "TAT transduction: the molecular mechanism and therapeutic prospects", Trends Mol Med, 13, pp.443~448 (2007)
  23. Brooks NA, "Cell penetrating peptides:application in vaccine delivery", Biochim Biophys Acta, 1805, pp.25~34 (2010)
  24. Woo SJ, "Effects of low doses of Tat-PIM2 protein against hippocampal neuronal cell survival", J Neurol Sci, 358, pp.226~235 (2015)
  25. Kim MJ, "Transduced PEP-1-PON1 proteins regulate microglial activation and dopaminergic neuronal death in a Parkinson's disease model", Biomaterials, 64, pp.45~56 (2015)
  26. Jeong HJ, "Neuroprotective effect of PEP-1-peroxiredoxin2 on CA1 regions in the hippocampus against ischemic insult", Biochim Biophys Acta, 1840, pp.2321~2330 (2014)
  27. Luo L, "Recombinant protein glutathione S-transferases P1 attenuates inflammation in mice", Mol Immunol, 46, pp.848~857 (2009)
  28. Townsend DM, "Novel role for glutathione S-transferase pi: regulator of protein S-glutathionylation following oxidative and nitrosative stress", J Biol Chem, 284, pp.436~445 (2009)
  29. Ramsey JD, "Cell-penetrating peptides transport therapeutics into cells", Pharmacol Therapeut, 154, pp.78~86 (2015)
  30. Dietz, "Cell penetrating peptide technology to delivery chaperones, associated factors in diseases, basic research", Curr Pharm Biotechnol, 11, pp.167~174 (2010)
  31. Lei B, "Measurement of the extracellular H2O2 in the brain by microdialysis", Brain Res Brain Res Protoc, 3, pp.33~36 (1998)
  32. Liu D, "Elevation of hydrogen peroxide after spinal cord injury detected by using the Fenton reaction", Free Radic Biol Med, 27, pp.478~482 (1999)
  33. Zhou L, "Glutathion-S-transferase π inhibits As2O3-induced apoptosis in lymphoma cells: involvement of hydrogen peroxided catabolism", Blood, 105, pp.1198~1203 (2005)
  34. Ohashi M, "Hydrogen peroxide modulates synaptic transmission in ventral horn neurons of the rat spinal cord", J Physiol, 594, pp.115~134 (2016)
  35. Prasad SS, "Neuroprotection induced in vitro by ischemic preconditioning, postconditioning: modulation of apoptosis and PI3K–Akt pathways", J Mol Neurosci, 43, pp.428~442 (2011)
  36. Adler V, "Regulation of JNK signaling by GSTpi", EMBO J, 18, pp.1321~1334 (1999)
  37. Kwon SH, "The neuroprotective effects of Lonicera japonica THUNB. against hydrogen peroxide-induced apoptosis via phosphorylation of MAPKs, PI3K", Akt in SH-SY5Y cells, 2011, pp.49, 1011~1019
  38. Higgins GC, "Oxidative stress: emerging mitochondrial, cellular themes and variations in neuronal injury", J Alzheimers Dis, 20, pp.453~473 (2010)
  39. Choi WS, "Characterization of MPP＋-induced cell death in a dopaminergic neuronal cell line : role of macromolecule synthesis, cytosolic calcium, caspase, and Bcl-2-related proteins", Exp Neurol, 159, pp.274~282 (1999)
  40. Fuenzalida K, "Peroxisome proliferator-activated receptor gamma up-regulates the Bcl-2 anti-apoptotic protein in neurons, induces apoptosis", J Biol Chem, 282, pp.37006~37015 (2007)
  41. Castro-Caldas M, "Glutathion S-transferase pi regulates UV-induced JNK signaling in SH-SY5Y neuroblastoma cells", Neurosci Lett, 451, pp.241~245 (2009)
  42. Castro-Caldas M, "GSTpi expression in MPTP-induced dopaminergic neurodegeneration of C57BL", 6 mouse midbrain and striatum, 2009, pp.38, 114~127
  43. Bradford MA, "A rapid, sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding", Anal Biochem, 72, pp.248~254 (1976)
  44. Kim YN, "Neuroprotective effects of PEP-1-carbonyl reductase 1 against oxidative stress-induced ischemic neuronal cell damage", Free Radic Biol Med, 69, pp.181~196 (2014)

관련 논문AI추천

Protective effects of Tat-NQO1 against oxidative stress-induced HT-22 cell damage, and ischemic injury in animals김대원, 김덕수, 박정환, 박진서, 신민재, 안은희, 여은지, 음원식, 이치헌, 정석영, 조성우, 조수빈, 조효상, 최수영, 최연주, 한규형, Hyeon Ji Yeo생화학분자생물학회 · BMB Reports2016 · 제49권 제11호Tat-Thioredoxin-like protein 1 attenuates ischemic brain injury by regulation of MAPKs and apoptosis signaling김덕수, 최수영, Dae Won Kim, Eun Jeong Sohn, Eun Ji Yeo, Gi Soo Youn, Hyeon Ji Yeo, Hyun Ju Cha, Hyun Jung Kwon, Jung Hwan Park, Lee Re Lee, Min Jea Shin, Na Yeon Kim, Oh-Shin Kwon, Sung-Woo Cho, Su Yeon Kwon, Won Sik Eum, Yong-Jun Cho, Yu Ran Lee생화학분자생물학회 · BMB Reports2023 · 제56권 제4호Tat-GSTpi suppresses inflammatory responses by regulating ROS/MAPKs/apoptosis signaling pathwaysChan Hee Lee, Dae Won Kim, Duk-Soo Kim, Eun Ji Yeo, Gi Soo Youn, Hyeon Ji Yeo, Hyun Jung Kwon, Jong Kook Park, Jung Hwan Park, Keun Wook Lee, Lee Re Lee, Min Jea Shin, Na Yeon Kim, Soo Young Choi, Su Min Kim, Su Yeon Kwon, Won Sik Eum, Yeon Joo Choi생화학분자생물학회 · BMB Reports2025 · 제58권 제6호Protective effects of PEP-1-Catalase on stress-induced cellular toxicity and MPTP-induced Parkinson’s disease권혁일, 김대원, 김덕수, 박진서, 손은정, 신민재, 안은희, 엄선애, 음원식, 전수정, 정석영, 조성우, 조효상, 최수영, 한규형생화학분자생물학회 · BMB Reports2015 · 제48권 제7호Neuroprotective effects of the antioxidant action of 2-cyclopropylimino-3-methyl-1,3-thiazoline hydrochloride against ischemic neuronal damage in the brain김대원, 김덕수, 김은아, 조성우, 최수영, 하성철, 한아름생화학분자생물학회 · BMB Reports2013 · 제46권 제7호Fenobam promoted the neuroprotective effect of PEP-1-FK506BP following oxidative stress by increasing its transduction efficiency김대원, 김덕수, 박은영, 박종훈, 박진서, 신민재, 안은희, 엄선애, 음원식, 조성우, 조효상, 최수영, 황현숙생화학분자생물학회 · BMB Reports2013 · 제46권 제11호

이 간행물의 다른 논문 (7건)

A novel model of THO/TREX loading onto target RNAs in metazoan gene expression허준호, 정연두pp. 355-356Merlin, a regulator of Hippo signaling, regulates Wnt/β-catenin signaling김소영, 조익훈pp. 357-358Forensic DNA methylation profiling from evidence material for investigative leads이환영, 이숭덕, 신경진pp. 359-369Induction of MAP kinase phosphatase 3 through Erk/MAP kinase activation in three oncogenic Ras (H-, K- and N-Ras)-expressing NIH/3T3 mouse embryonic fibroblast cell linesSen Wang, 박영일, 강나나, 허선진, 구재형pp. 370-375c-Jun N-terminal Kinase (JNK) induces phosphorylation of amyloid precursor protein (APP) at Thr668, in okadaic acid-induced neurodegeneration김동호, 김현주, 김나영, 윤승용, 소상필, 안지환pp. 376-381
원문 보기