Peroxiredoxin I deficiency attenuates phagocytic capacity of macrophage in clearance of the red blood cells damaged by oxidative stress
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The role of peroxiredoxin (Prx) I as an erythrocyte antioxidant defense in red blood cells (RBCs) is controversial. Here we investigated the function of Prx I by using Prx I–⁄– and Prx I/II–⁄–mice. Prx I–⁄– mice exhibited a normal blood profile. However,Prx I/II–⁄– mice showed more significantly increased Heinz body formation as compared with Prx II–⁄– mice. The clearance rate of Heinz body-containing RBCs in Prx I–⁄– mice decreased significantly through the treatment of aniline hydrochloride (AH)compared with wild-type mice. Prx I deficiency decreased the phagocytic capacity of macrophage in clearing Heinz bodycontaining RBCs. Our data demonstrate that Prx I deficiency did not cause hemolytic anemia, but showed that further increased hemolytic anemia symptoms in Prx II–⁄– mice by attenuating phagocytic capacity of macrophage in oxidative stress damaged RBCs, suggesting a novel role of Prx I in phagocytosis of macrophage. [BMB Reports 2012; 45(10): 560-564]
Abstract
The role of peroxiredoxin (Prx) I as an erythrocyte antioxidant defense in red blood cells (RBCs) is controversial. Here we investigated the function of Prx I by using Prx I–⁄– and Prx I/II–⁄–mice. Prx I–⁄– mice exhibited a normal blood profile. However,Prx I/II–⁄– mice showed more significantly increased Heinz body formation as compared with Prx II–⁄– mice. The clearance rate of Heinz body-containing RBCs in Prx I–⁄– mice decreased significantly through the treatment of aniline hydrochloride (AH)compared with wild-type mice. Prx I deficiency decreased the phagocytic capacity of macrophage in clearing Heinz bodycontaining RBCs. Our data demonstrate that Prx I deficiency did not cause hemolytic anemia, but showed that further increased hemolytic anemia symptoms in Prx II–⁄– mice by attenuating phagocytic capacity of macrophage in oxidative stress damaged RBCs, suggesting a novel role of Prx I in phagocytosis of macrophage. [BMB Reports 2012; 45(10): 560-564]
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