microRNA-214-mediated UBC9 expression in glioma
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It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9),the unique enzyme2 in the sumoylation pathway, is up-regulated in many cancers. However, the expression and regulation of UBC9 in glioma remains unknown. In this study, we found that Ubc9 was up-regulated in glioma tissues and cell lines compared to a normal control. UBC9 knockdown by small interfering RNA (siRNA) affected cell proliferation and apoptosis in T98G cells. Further experiments revealed that microRNA (miR)-214 directly targeted the 3' untranslated region (UTR) of UBC9 and that there was an inverse relationship between the expression levels of miR-214 and UBC9 protein in glioma tissues and cells. miR-214overexpression suppressed the endogenous UBC9 protein and affected T98G cell proliferation. These findings suggest that miR-214 reduction facilitates UBC9 expression and is involved in the regulation of glioma cell proliferation. [BMB Reports 2012; 45(11): 641-646]
Abstract
It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9),the unique enzyme2 in the sumoylation pathway, is up-regulated in many cancers. However, the expression and regulation of UBC9 in glioma remains unknown. In this study, we found that Ubc9 was up-regulated in glioma tissues and cell lines compared to a normal control. UBC9 knockdown by small interfering RNA (siRNA) affected cell proliferation and apoptosis in T98G cells. Further experiments revealed that microRNA (miR)-214 directly targeted the 3' untranslated region (UTR) of UBC9 and that there was an inverse relationship between the expression levels of miR-214 and UBC9 protein in glioma tissues and cells. miR-214overexpression suppressed the endogenous UBC9 protein and affected T98G cell proliferation. These findings suggest that miR-214 reduction facilitates UBC9 expression and is involved in the regulation of glioma cell proliferation. [BMB Reports 2012; 45(11): 641-646]
참고문헌 (27)
- Johnson, E. S., "Protein modification by SUMO", Ann. Rev. Biochem., 73, pp.355~382 (2004)
- Verger, A., "Modification with SUMO. A role in transcriptional regulation", EMBO Rep., 4, pp.137~142 (2003)
- Kerscher, O., "Modification of proteins by ubiquitin and ubiquitin-like proteins", Annu. Rev. Cell Dev. Biol., 22, pp.159~180 (2006)
- Capili, A. D., "Taking it step by step: mechanistic insights from structural studies of ubiquitin", ubiquitin-like protein modification pathways, 2007, pp.17, 726~735
- Geiss-Friedlander, R., "Concepts in sumoylation: a decade on", Nat. Rev., 8, pp.947~956 (2007)
- Bernier-Villamor, V., "Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin- conjugating enzyme Ubc9 and RanGAP1", Cell, 108, pp.345~356 (2002)
- Gostissa, M., "Activation of p53 by conjugation to the ubiquitin-like protein SUMO-1", EMBO J., 18, pp.6462~6471 (1999)
- Mo, Y. Y., "A role for Ubc9 in tumorigenesis", Oncogene, 24, pp.2677~2683 (2005)
- Zhu, S., "Ubc9 promotes breast cell invasion and metastasisin a sumoylation-independent manner", Oncogene, 29, pp.1763~1772 (2010)
- Moschos, S. J., "Expression analysis of Ubc9, the single small ubiquitin- like modifier (SUMO) E2 conjugating enzyme, in normal and malignant tissues", Hum. Pathol., 41, pp.1286~1298 (2010)
- Wu, F., "MicroRNA-mediated regulation of Ubc9 expressionin cancer cells", Clin. Cancer Res., 15, pp.1550~1557 (2009)
- Yu, F., "Mir-30 reduction maintains self-renewal and in-hibits apoptosis in breast tumor-initiating cells", Oncogene, 29, pp.4194~4204 (2010)
- Bartel, D. P., "MicroRNAs: genomics, biogenesis, mechanism, and function", Cell, 116, pp.281~297 (2004)
- Esquela-Kerscher, A., "Oncomirs - microRNAs with a role in cancer", Nat. Rev. Cancer, 6, pp.259~269 (2006)
- Furnari, F. B., "Malignant astrocytic glioma: genetics, biology, and paths to treatment", Genes Dev., 21, pp.2683~2710 (2007)
- Calin, G. A., "Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia", Proc. Natl. Acad. Sci. U.S.A., 99, pp.15524~15529 (2002)
- Calin, G. A., "A MicroRNA signature associated with prognosis and progression in chronic lymphocytic leukemia", New Engl. J. Med., 353, pp.1793~1801 (2005)
- van Jaarsveld, M. T., "MicroRNAs in ovarian cancer biology and therapy resistance", Int. J. Biochem. Cell Biol., 42, pp.1282~1290 (2010)
- Tombol, Z., "Integrative molecular bioinformatics study of human adrenocortical tumors: microRNA, tissue-specific target prediction, and pathway analysis", Endocr. Relat. Cancer, 16, pp.895~906 (2009)
- Yang, H., "MicroRNA expression profiling in human ovarian cancer: miR-214 induces cell survival and cisplatin resistance by targeting PTEN", Cancer Res., 68, pp.425~433 (2008)
- Magrelli, A., "Altered microRNA Expression Patterns in Hepatoblastoma Patients", Translat. Oncol., 2, pp.157~163 (2009)
- Qiang, R., "Plexin-B1 is a target of miR-214 in cervical cancer and promotes the growth and invasion of HeLa cells", Int. J. Biochem. Cell Biol., 43, pp.632~641 (2011)
- Lee, Y. B., "Twist-1 regulates the miR-199a", 214 cluster during development, 2009, pp.37, 123~128
- Derfoul, A., "Decreased microRNA-214 levels in breast cancer cells coincides with increased cell proliferation, invasion and accumulation of the Polycomb Ezh2 methyltransferase", Carcinogenesis, 32, pp.1607~1614 (2011)
- Chen, Y., "MicroRNA-21 down-regulates the expression of tumor suppressor PDCD4 in human glioblastoma cell T98G", Cancer Lett., 272, pp.197~205 (2008)
- Chen, C., "Real-time quantification of microRNAs by stem-loop RT-PCR", Nucleic Acids Res., 33, pp.e179~ (2005)
- Zhang, Y., "MicroRNA-128 inhibits glioma cells proliferation by targeting transcription factor E2F3a", J. Mol. Med., 87, pp.43~51 (2008)