Curcumin ameliorates TNF-α-induced ICAM-1 expression and subsequent THP-1 adhesiveness via the induction of heme oxygenase-1 in the HaCaT cells
1
조회
0
다운로드
7
인용
0
참고문헌
키워드
초록
Adhesion molecules such as ICAM-1 are important in the infiltration of leukocytes into the site of inflammation. In this study, we investigated the inhibitory effects of curcumin on ICAM-1 expression and monocyte adhesiveness as well as its underlying action mechanism in the TNF-α-stimulated keratinocytes. Curcumin induced expression of heme oxygenase-1(HO-1) in the human keratinocyte cell line HaCaT. In addition,curcumin induced Nrf2 activation in dose- and time-dependent manners in the HaCaT cells. Curcumin suppressed TNF-α-induced ICAM-1 expression and subsequent monocyte adhesion,which were reversed by the addition of tin protoporphyrin IX (SnPP), a specific inhibitor of HO-1, or HO-1knockdown using siRNA. Furthermore, Nrf2 knockdown using siRNA reversed the inhibitory effect of curcumin on the TNF-α-induced ICAM-1 expression and adhesion of monocytes to keratinocytes. These results suggest that curcumin may exert its anti-inflammatory activity by suppressing the TNF-α-induced ICAM-1 expression and subsequent monocyte adhesion via expression of HO-1 in the keratinocytes.
Abstract
Adhesion molecules such as ICAM-1 are important in the infiltration of leukocytes into the site of inflammation. In this study, we investigated the inhibitory effects of curcumin on ICAM-1 expression and monocyte adhesiveness as well as its underlying action mechanism in the TNF-α-stimulated keratinocytes. Curcumin induced expression of heme oxygenase-1(HO-1) in the human keratinocyte cell line HaCaT. In addition,curcumin induced Nrf2 activation in dose- and time-dependent manners in the HaCaT cells. Curcumin suppressed TNF-α-induced ICAM-1 expression and subsequent monocyte adhesion,which were reversed by the addition of tin protoporphyrin IX (SnPP), a specific inhibitor of HO-1, or HO-1knockdown using siRNA. Furthermore, Nrf2 knockdown using siRNA reversed the inhibitory effect of curcumin on the TNF-α-induced ICAM-1 expression and adhesion of monocytes to keratinocytes. These results suggest that curcumin may exert its anti-inflammatory activity by suppressing the TNF-α-induced ICAM-1 expression and subsequent monocyte adhesion via expression of HO-1 in the keratinocytes.
참고문헌 (31)
- Gröne, A., "Keratinocytes and cytokines, Vet", Immunol. Immunopathol, 88, pp.1~12 (2002)
- Dustin, M. L., "Adhesion of T lymphoblasts to epidermal keratinocytes is regulated by interferon gamma and is mediated by intercellular adhesion molecule 1 (ICAM-1)", J. Exp. Med, 167, pp.1323~1340 (1988)
- Barker, J. N., "Marked synergism between tumor necrosis factor-alpha and interferon-gamma in regulation of keratinocyte-derived adhesion molecules and chemotactic factors", J. Clin. Invest, 85, pp.605~608 (1990)
- Krutmann, J., "Tumor necrosis factor beta and ultraviolet radiation are potent regulators of human keratinocyte ICAM-1 expression", J. Invest. Dermatol, 95, pp.127~131 (1990)
- Zhou, H., "The targets of curcumin", Curr. Drug. Targets, 12, pp.332~347 (2011)
- Thangapazham, R. L., "Beneficial role of curcumin in skin diseases", Adv. Exp. Med. Biol, 595, pp.343~357 (2007)
- Cho, J. W., "Curcumin attenuates the expression of IL-1beta, IL-6, and TNF-alpha as well as cyclin E in TNF-alpha-treated HaCaT cells; NFkappaB and MAPKs as potential upstream targets", Int. J. Mol. Med, 19, pp.469~474 (2007)
- 조재위, "Curcumin inhibits the expression of COX-2 in UVB-irradiated human keratinocytes (HaCaT) by inhibiting activation of AP-1: p38 MAP kinase and JNK as potential upstream targets", Experimental and Molecular Medicine, 37(3), pp.186~192 (2005), 생화학분자생물학회
- Grandjean-Laquerriere, A., "Relative contribution of NF-kappaB and AP-1 in the modulation by curcumin and pyrrolidine dithiocarbamate of the UVB-induced cytokine expression by keratinocytes", Cytokine, 18, pp.168~177 (2002)
- Lee, J. H., "Curcumin, a constituent of curry, suppresses IgE-mediated allergic response and mast cell activation at the level of Syk", J. Allergy Clin. Immunol, 121, pp.1225~1231 (2008)
- Ryter, S. W., "Heme oxygenase- 1", carbon monoxide: from basic science to therapeutic applications, 2006, pp.86, 583~650
- Listopad, J., "Heme oxygenase-1 inhibits T cell-dependent skin inflammation and differentiation and function of antigen-presenting cells", Exp. Dermatol, 16, pp.661~670 (2007)
- Kirino, M., "Heme oxygenase 1 attenuates the development of atopic dermatitis-like lesions in mice: implications for human disease", J. Allergy. Clin. Immunol, 122, pp.290~297 (2008)
- Pae, H. O., "Heme oxygenase-1 attenuates contact hypersensitivity induced by 2,4-dinitrofluorobenzene in mice", Immunopharmacol Immunotoxicol, 30, pp.207~216 (2008)
- Rattan, S. I., "Heat stress and hormetin-induced hormesis in human cells: effects on aging, wound healing, angiogenesis, and differentiation", Dose Response, 7, pp.90~103 (2009)
- Lima, C. F., "Curcumin induces heme oxygenase-1 in normal human skin fibroblasts through redox signaling: relevance for anti-aging intervention", Mol. Nutr. Food Res, 55, pp.430~442 (2011)
- Berge, U., "Hormetic modulation of differentiation of normal human epidermal keratinocytes undergoing replicative senescence in vitro", Exp. Gerontol, 43, pp.658~662 (2008)
- Motterlini, R., "Curcumin, an antioxidant and anti-inflammatory agent, induces heme oxygenase-1 and protects endothelial cells against oxidative stress", Free Radic. Biol. Med, 28, pp.1303~1312 (2000)
- Kim, A. N., "Up-regulation of heme oxygenase-1 expression through CaMKII-ERK1", 2-Nrf2 signaling mediates the anti-inflammatory effect of bisdemethoxycurcumin in LPS-stimu lated macrophages, 2010, pp.49, 323~331
- Hsu, H. Y., "Heme oxygenase-1 mediates the anti-inflammatory effect of Curcumin within LPS-stimulated human monocytes", J. Cell Physiol, 215, pp.603~612 (2008)
- Kumar, A., "Curcumin (Diferuloylmethane) inhibition of tumor necrosis factor (TNF)-mediated adhesion of monocytes to endothelial cells by suppression of cell surface expression of adhesion molecules and of nuclear factor- kappaB activation", Biochem. Pharmacol, 55, pp.775~783 (1998)
- Gupta, B., "Curcuma longa inhibits TNF-alpha induced expression of adhesion molecules on human umbilical vein endothelial cells", Int. J. Immunopharmacol, 21, pp.745~757 (1999)
- Bito, T., "Pine bark extract pycnogenol downregulates IFN-gamma-induced adhesion of T cells to human keratinocytes by inhibiting inducible ICAM-1 expression", Free Radic. Biol. Med, 28, pp.219~227 (2000)
- Kostyuk, V., "The promise of plant polyphenols as the golden standard skin anti-inflammatory agents", Curr. Drug. Metab, 11, pp.414~424 (2010)
- Olszanecki, R., "The role of haem oxygenase-1 in the decrease of endothelial intercellular adhesion molecule-1 expression by curcumin", Basic Clin. Pharmacol. Toxicol, 101, pp.411~415 (2007)
- Seo, W. Y., "Celastrol suppresses IFN-gamma-induced ICAM-1 expression and subsequent monocyte adhesiveness via the induction of heme oxygenase-1 in the HaCaT cells.", Biochem. Biophys. Res. Commun, 398, pp.140~145 (2010)
- Seo, W. Y., "Celastrol induces expression of heme oxygenase-1 through ROS", Nrf2, Biochem. Biophys. Res. Commun, pp.2011, 407[DOI]
- Paine, A., "Signaling to heme oxygenase-1 and its anti-inflammatory therapeutic potential", Biochem. Pharmacol, 80, pp.1895~1903 (2010)
- Ha Yong Song, "Suppression of TNF-alpha-induced MMP-9 expression by a cell-permeable superoxide dismutase in keratinocytes", BMB Reports, 44(7), pp.462~467 (2011), 생화학분자생물학회
- 이선화, "Suppression of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation in mice by transduced Tat-Annexin protein", BMB Reports, 45(6), pp.354~359 (2012), 생화학분자생물학회[DOI]
- 박진서, "The Enhanced Monocyte Adhesiveness after UVB Exposure Requires ROS and NF-κB Signaling in Human Keratinocyte", BMB reports, 39(5), pp.618~625 (2006), 한국생화학분자생물학회
