Wogonin inhibits Cytokine-induced TARC/CCL17 Expression by Suppression of NF-κB activation via p38 MAP kinase Signalning Pathways in HaCaT Keratinocytes
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초록
Thymus and activation-regulated chemokine (TARC/CCL-17), produced by keratinocytes, is a CC chemokine known to selectively Th2 type T cells via CCR4+ and is implicated in the development of atopic dermatitis (AD). TARC/CCL17 expression was induced by cytokines such as tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). We recently found that the wogonin, a flavone isolated from Scutellaria baicalensis, suppressed TARC expression via heme oxygenase 1 (HO1) in human keratinocytes induced with mite antigen. However, little is known about the inhibitory mechanism of wogonin on TARC/CCL-17 expression stimulated with cytokines. To investigate the inhibitory mechanism, I determined the inhibitory effects of wogonin on the activation of nuclear factor-κB (NF-κB) and IκBα phosphorylation, and also examined the activation of p38 MAP kainase in HaCaT keratinocytes stimulated with TNF-α and IFN-γ. Wogonin inhibited NF-κB-DNA complex, NF-κB binding activity, and the phosphorylation of IκBα in a dose dependent manner. Wogonin also inhibited the translocation of NF-κB from cytosol to nucleus. Moreover, the phosphorylation of of p38 MAP kinase in the TNF-α and IFN-γ-stimulated HaCaT keratinocytes were suppressed by wogonin in a dose dependent manner. These results suggest that wogonin may inhibit cytokine-induced NF-κB activation by IκBα degradation via suppression of p38 MAP kinase signaling pathway in keratinocytes and modulation of wogonin signaling pathway may be beneficial for the treatment of AD.
Abstract
Thymus and activation-regulated chemokine (TARC/CCL-17), produced by keratinocytes, is a CC chemokine known to selectively Th2 type T cells via CCR4+ and is implicated in the development of atopic dermatitis (AD). TARC/CCL17 expression was induced by cytokines such as tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). We recently found that the wogonin, a flavone isolated from Scutellaria baicalensis, suppressed TARC expression via heme oxygenase 1 (HO1) in human keratinocytes induced with mite antigen. However, little is known about the inhibitory mechanism of wogonin on TARC/CCL-17 expression stimulated with cytokines. To investigate the inhibitory mechanism, I determined the inhibitory effects of wogonin on the activation of nuclear factor-κB (NF-κB) and IκBα phosphorylation, and also examined the activation of p38 MAP kainase in HaCaT keratinocytes stimulated with TNF-α and IFN-γ. Wogonin inhibited NF-κB-DNA complex, NF-κB binding activity, and the phosphorylation of IκBα in a dose dependent manner. Wogonin also inhibited the translocation of NF-κB from cytosol to nucleus. Moreover, the phosphorylation of of p38 MAP kinase in the TNF-α and IFN-γ-stimulated HaCaT keratinocytes were suppressed by wogonin in a dose dependent manner. These results suggest that wogonin may inhibit cytokine-induced NF-κB activation by IκBα degradation via suppression of p38 MAP kinase signaling pathway in keratinocytes and modulation of wogonin signaling pathway may be beneficial for the treatment of AD.
참고문헌 (45)
- "Cosmetic dermatology: Principles & practice", pp.3~8 (2002), McGraw-Hill
- "New insights into atopic dermatitis", 113, pp.651~657 (2004)
- "Apoptosis in tissue inflammation and allergic disease", 16, pp.717~723 (2004)
- "Biphasic response against aeroallergen in atopic dermatitis showing a switch from an initial TH2 response to a TH1 response in situ: an immunocytochemical study", 97, pp.828~837 (1996)
- "T cells and T cell-derived cytokines as pathogenic factors in the nonallergic form of atopic dermatitis", 113, pp.628~634 (1999)
- "T cell-mediated Fas-induced keratinocyte apoptosis plays a key pathogenetic role in eczematous dermatitis", 106, pp.25~35 (2000)
- "Targeting keratinocyte apoptosis in the treatment of atopic dermatitis and allergic contact dermatitis", 108, pp.839~846 (2001)
- "A second step of chemotaxis after transendothelial migration: keratinocytes undergoing apoptosis release IFN-γ inducible protein 10, monokine induced by IFN-γ, and IFN-γ-inducible α-chemoattractant for T cell chemotaxis toward epidermis in atopic dermatitis", 171, pp.1078~1084 (2003)
- "The biology of chemokines and their receptors", 18, pp.217~242 (2000)
- "Chemokines: a new classification system and their role in immunity", 12, pp.121~127 (2004)
- "Macrophage-derived chemokine production by activated human T cells in vitro and in vivo: preferential association with the production of type 2 cytokines", 30, pp.204~210 (2000)
- "Distinct patterns and kinetics of chemokine production regulate dendritic cell function", 29, pp.1617~1625 (1999)
- "Overexpression of Th2-specific chemokines in NC", Nga mice exhibiting atopic dermatitis-like lesions, 1999, pp.104, 1097~1105
- "Thymus and activation-regulated chemokine in atopic dermatitis: serum thymus and activation-regulated chemokine level is closely related with disease activity", 107, pp.535~541 (2001)
- "Chemokines and allergic disease", 104, pp.723~742 (1999)
- "Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4", 273, pp.1764~1768 (1998)
- "Differential expression of chemokine receptors and chemotactic responsiveness of type 1 T helper cells (Th1s) and Th2s", 187, pp.129~134 (1998)
- Lim, B.O, "Efficacy of wogonin in the production of immunoglobulins and cytokines by mesenteric lymph node lymphocytes in mouse colitis induced with dextran sulfate sodium", 68, pp.2505~2511 (2004)
- Lim, B.O, "Effect of the flavonoid components obtained from Scutellaria radix on the histamine, immunoglobulin E and lipid peroxidation of spleen lymphocytes of Sprague-Dawley rats", 67, pp.1126~1129 (2003)
- Chang, Y.L, "Chinese herbal remedy wogonin inhibits monocyte chemotactic protein-1 gene expression in human endothelial cells", 60, pp.507~513 (2001)
- Lee, S.O, "Wogonin suppresses TNF-alpha-induced MMP-9 expression by blocking the NF-kappaB activation via MAPK signaling pathways in human aortic smooth muscle cells", 351, pp.118~125 (2006)
- "Wogonin suppresses TARC expression induced by mite antigen via heme oxygenase 1 in human keratinocytes. Suppressive effect of wogonin on mite antigen-induced TARC expression", 46, pp.31~40 (2007)
- "IFN-gamma-inducible expression of thymus and activation-regulated chemokine", CCL17 and macrophage-derived chemokine, pp.2002, 14[DOI]
- "Assay for redoxsensitive transcription factor", 319, pp.585~602 (2000)
- "A Th2 chemokine, TARC, produced by keratinocytes may recruit CLA+CCR4+ lymphocytes into lesional atopic dermatitis skin", 115, pp.640~646 (2001)
- "Roxithromycin downmodulates Th2 chemokine production by keratinocytes and chemokine receptor expression on Th2 cells: its dual inhibitory effects on the ligands and the receptors", 228, pp.27~33 (2004)
- "Mechanism of thymus- and activation-regulated chemokine (TARC)", CCL17 production and its modulation by roxithromycin, 2005, pp.125, 491~498
- "ERK1", 2 regulates epidermal chemokine expression and skin inflammation, 2005, pp.174, 5047~5056
- "International union of pharmacology. XXII. Nomenclature for chemokine receptors", 52, pp.145~176 (2000)
- "The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4", 1272, pp.15036~15042 (1997)
- "Selective recruitment of CCR4bearing Th2 cells toward antigen-presenting cells by the CC chemokine thymus and activationregulated chemokine and macrophage-derived chemokine", 11, pp.81~88 (1999)
- "The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells", 400, pp.776~880 (1999)
- "TARC: novel mediator of allergic inflammation", 31, pp.1809~1812 (2001)
- "Effect of interleukin-1beta and tumor necrosis factor-alpha on gene expression in human endothelial cells", 284, pp.C1577~C1583 (2003)
- "Glucosamine inhibits IL-1betainduced NFkappaB activation in human osteoarthritic chondrocytes", 11, pp.290~298 (2003)
- "Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases", 336, pp.1066~1071 (1997)
- "NF-kappaB regulation in the immune system", 2, pp.725~734 (2002)
- "Suppression of NF-kappaB-dependent proinflammatory gene expression in human RPE cells by a proteasome inhibitor", 40, pp.477~486 (1999)
- "Transducing signals from antigen receptors to nuclear factor kappaB", 193, pp.93~100 (2003)
- "Tanshinone IIA inhibits LPS-induced NF-kappaB activation in RAW 264.7 cells: possible involvement of the NIK-IKK, ERK1", 2, p38 and JNK pathways, 2006, pp.542, 1~7
- "LPS induction of gene expression in human monocytes", 13, pp.85~94 (2001)
- "Extracellular signal regulated kinase and p38 subgroups of mitogen-activated protein kinases regulate inducible nitric oxide synthase and tumor necrosis factor-alpha gene expression in endotoxin-stimulated primary glial cultures", 18, pp.1633~1641 (1998)
- "Mechanism of thymus- and activation-regulated chemokine (TARC)", CCL17 production and its modulation by roxithromycin, 2005, pp.125, 491~498
- "Cross-talk between ERK and p38 MAPK mediates selective suppression of pro-inflammatory cytokines by transforming growth factor-beta", 277, pp.14884~14893 (2002)
- "Inhibition of iNOS gene expression by quercetin is mediated by the inhibition of IkappaB kinase, nuclear factor-kappa B and STAT1, and depends on heme oxygenase-1 induction in mouse BV-2 microglia", 521, pp.9~20 (2005)
