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SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion

김동은|장하나|하지연|조승희|정학수|최남정|조성찬|Yongchao Liu|심해홍|문희금|Tiing Jen Loh|오자경|Jianhua Zhou|Michael B. Ye
생화학분자생물학회 (Korean Society Of Biochemistry And Molecular Biology)|2017.08|BMB Reports|제50권 제8호|pp. 423-428

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참고문헌

키워드

Intron retentionSRSF2Exon exclusionPre-mRNA splicing
주제분류화학

초록

SRSF2, a Serine-Arginine rich (SR) protein, is a splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron splicing to suppress cassette exon inclusion in SMN premRNA. Through a serial mutagenesis, we demonstrate that a 10 nt RNA sequence surrounding the branch-point (BP), is important for SRSF2-mediated inhibition of cassette exon inclusion through directly interacting with SRSF2. We conclude that SRSF2 inhibits intron splicing to promote exon exclusion.

Abstract

SRSF2, a Serine-Arginine rich (SR) protein, is a splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron splicing to suppress cassette exon inclusion in SMN premRNA. Through a serial mutagenesis, we demonstrate that a 10 nt RNA sequence surrounding the branch-point (BP), is important for SRSF2-mediated inhibition of cassette exon inclusion through directly interacting with SRSF2. We conclude that SRSF2 inhibits intron splicing to promote exon exclusion.

참고문헌 (34)

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관련 논문AI추천

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