Investigation of the effect of SRSF9 overexpression on HIV-1 production
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Serine-arginine-rich splicing factors (SRSFs) are members of RNAprocessing proteins in the serine-arginine-rich (SR) family thatcould regulate the alternative splicing of the human immunodeficiencyvirus-1 (HIV-1). Whether SRSF9 has any effect onHIV-1 regulation requires elucidation. Here, we report for thefirst time the effects and mechanisms of SRSF9 on HIV-1 regulation. The overexpression of SRSF9 inhibits viral productionand infectivity in both HEK293T and MT-4 cells. Deletion analysisof SRSF9 determined that the RNA regulation motif domainof SRSF9 is important for anti-HIV-1 effects. Furthermore, overexpressionof SRSF9 increases multiple spliced forms of viralmRNA, such as Vpr mRNA. These data suggest that SRSF9overexpression inhibits HIV-1 production by inducing the imbalancedHIV-1 mRNA splicing that could be exploited furtherfor a novel HIV-1 therapeutic molecule.
Abstract
Serine-arginine-rich splicing factors (SRSFs) are members of RNAprocessing proteins in the serine-arginine-rich (SR) family thatcould regulate the alternative splicing of the human immunodeficiencyvirus-1 (HIV-1). Whether SRSF9 has any effect onHIV-1 regulation requires elucidation. Here, we report for thefirst time the effects and mechanisms of SRSF9 on HIV-1 regulation. The overexpression of SRSF9 inhibits viral productionand infectivity in both HEK293T and MT-4 cells. Deletion analysisof SRSF9 determined that the RNA regulation motif domainof SRSF9 is important for anti-HIV-1 effects. Furthermore, overexpressionof SRSF9 increases multiple spliced forms of viralmRNA, such as Vpr mRNA. These data suggest that SRSF9overexpression inhibits HIV-1 production by inducing the imbalancedHIV-1 mRNA splicing that could be exploited furtherfor a novel HIV-1 therapeutic molecule.
참고문헌 (33)
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