Discovery of 14-3-3 zeta as a potential biomarker for cardiac hypertrophy
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Acute myocardial infarction (AMI) is a multifaceted syndromeinfluenced by the functions of various extrinsic and intrinsicpathways and pathological processes, which can be detectedin circulation using biomarkers. In this study, we investigatedthe secretome protein profile of induced-hypertrophy cardiomyocytesto identify next-generation biomarkers for AMI diagnosisand management. Hypertrophy was successfully induced inimmortalized human cardiomyocytes (T0445) by 200 nM ET-1and 1 μM Ang II. The protein profiles of hypertrophied cardiomyocytesecretomes were analyzed by nano-liquid chromatographywith tandem mass spectrometry and differentially expressedproteins that have been identified by Ingenuity PathwayAnalysis. The levels of 32 proteins increased significantly(＞1.4 fold), whereas 17 proteins (＜0.5 fold) showed a rapiddecrease in expression. Proteomic analysis showed significantupregulation of six 14-3-3 protein isoforms in hypertrophiedcardiomyocytes compared to those in control cells. Multi-reactionmonitoring results of human plasma samples showed that14-3-3 protein-zeta levels were significantly elevated in patientswith AMI compared to those of healthy controls. These findingselucidated the role of 14-3-3 protein-zeta in cardiac hypertrophyand cardiovascular disorders and demonstrated its potential asa novel biomarker and therapeutic strategy.
Abstract
Acute myocardial infarction (AMI) is a multifaceted syndromeinfluenced by the functions of various extrinsic and intrinsicpathways and pathological processes, which can be detectedin circulation using biomarkers. In this study, we investigatedthe secretome protein profile of induced-hypertrophy cardiomyocytesto identify next-generation biomarkers for AMI diagnosisand management. Hypertrophy was successfully induced inimmortalized human cardiomyocytes (T0445) by 200 nM ET-1and 1 μM Ang II. The protein profiles of hypertrophied cardiomyocytesecretomes were analyzed by nano-liquid chromatographywith tandem mass spectrometry and differentially expressedproteins that have been identified by Ingenuity PathwayAnalysis. The levels of 32 proteins increased significantly(＞1.4 fold), whereas 17 proteins (＜0.5 fold) showed a rapiddecrease in expression. Proteomic analysis showed significantupregulation of six 14-3-3 protein isoforms in hypertrophiedcardiomyocytes compared to those in control cells. Multi-reactionmonitoring results of human plasma samples showed that14-3-3 protein-zeta levels were significantly elevated in patientswith AMI compared to those of healthy controls. These findingselucidated the role of 14-3-3 protein-zeta in cardiac hypertrophyand cardiovascular disorders and demonstrated its potential asa novel biomarker and therapeutic strategy.
참고문헌 (30)
- Thrift AG, "Global stroke statistics", Int J Stroke, 12, pp.13~32 (2017)
- Maron BJ, "Clinical course and management of hypertrophic cardiomyopathy", NJEM, 379, pp.655~668 (2018)
- Rowin EJ, "The hypertrophic cardiomyopathy phenotype viewed through the prism of multimodality imaging : clinical and etiologic implications", Cardiovasc Imag, 13, pp.2002~2016 (2020)
- Laflamme MA, "Heart regeneration", Nature, 473, pp.326~335 (2011)
- Garg P, "Cardiac biomarkers of acute coronary syndrome : from history to highsensitivity cardiac troponin", Intern Emerg Med, 12, pp.147~155 (2017)
- Babuin L, "Troponin : the biomarker of choice for the detection of cardiac injury", CMAJ, 173, pp.1191~1202 (2005)
- Mueller T, "Diagnostic accuracy of B type natriuretic peptide and amino terminal proBNP in the emergency diagnosis of heart failure", Heart, 91, pp.606~612 (2005)
- Toldo S, "The NLRP3 inflammasome in acute myocardial infarction", Nat Rev Cardiol, 15, pp.203~214 (2018)
- 강민정, "Obscurin and Clusterin Elevation in Serum of Acute Myocardial Infarction Patients", Bulletin of the Korean Chemical Society, 41(3), pp.266~273 (2020), 대한화학회[DOI]
- Chan D, "Biomarkers in acute myocardial infarction", BMC Med, 8, pp.1~11 (2010)
- Watkins SJ, "The H9C2 cell line and primary neonatal cardio myocyte cells show similar hypertrophic responses in vitro", In Vitro Cell Dev Biol Anim, 47, pp.125~131 (2011)
- Manso AM, "Talin1 has unique expression versus talin 2 in the heart and modifies the hypertrophic response to pressure overload", J Biol Chem, 288, pp.4252~4264 (2013)
- Bandaru S, "Filamin A regulates cardiovascular remodeling", Int J Mol Sci, 22, pp.6555~ (2021)
- Lee SY, "High circulating follistatin-like protein 1 as a biomarker of a metabolically unhealthy state", Endocr J, 66, pp.241~251 (2019)
- Paszek E, "Evaluation of profilin 1 as a biomarker in myocardial infarction", Eur Rev Med Pharmacol Sci, 24, pp.8112~8116 (2020)
- Yang D, "Downregulation of profilin-1 expression attenuates cardiomyocytes hypertrophy and apoptosis induced by advanced glycation end products in H9c2 cells", Biomed Res Int, 2017, pp.9716087~ (2017)
- Klopsch C, "Vimentin-induced cardiac mesenchymal stem cells proliferate in the acute ischemic myocardium", Cells Tissues Organs, 206, pp.35~45 (2018)
- Gong DH, "Secretory vimentin is associated with coronary artery disease in patients and induces atherogenesis in ApoE(-", -)mice, 2019, pp.283, 9~16
- Zhou X, "Filamin B deficiency in mice results in skeletal malformations and impaired microvascular development", Proc Natl Acad Sci USA, 104, pp.3919~3924 (2007)
- Carrascosa JL, "Structural comparison of prokaryotic and eukaryotic chaperonins", Micron, 32, pp.43~50 (2001)
- Li JY, "Dynamic distribution of linker histone H1. 5 in cellular differentiation", PLoS Genet, 8, pp.e1002879~ (2012)
- Bauden M, "Histone profiling reveals the H1. 3 histone variant as a prognostic biomarker for pancreatic ductal adenocarcinoma", BMC Cancer, 17, pp.810~ (2017)
- Silacci P, "Gelsolin superfamily proteins : key regulators of cellular functions", Cell Mol Life Sci, 61, pp.2614~2623 (2004)
- Severino V, "Analysis of secreted proteins", Methods Mol Biol, 1002, pp.37~60 (2013)
- Hernandez-Ruiz L, "Organellar proteomics of human platelet dense granules reveals that 14-3-3ζ is a granule protein related to atherosclerosis", J Proteome Res, 6, pp.4449~4457 (2007)
- Thompson WC, "14-3-3 protein regulation of excitation-contraction coupling", Pflug Arch Eur J Phy, 474, pp.267~279 (2022)
- Mugabo Y, "14-3-3ζ Constrains insulin secretion by regulating mitochondrial function in pancreatic β cells", JCI insight, 7, pp.e156378~ (2022)
- Zhou Q, "The hippo pathway in heart development, regeneration, and diseases", Circ Res, 116, pp.1431~1447 (2015)
- Zhou W, "How hippo signaling pathway modulates cardiovascular development and diseases", J Immuno Res, 2018, pp.3696914~ (2018)
- 서지명, "Regulation of Hippo signaling by actin remodeling", BMB Reports, 51(3), pp.151~156 (2018), 생화학분자생물학회