Photobiomodulation therapy activates YAP and triggers proliferation and dedifferentiation of Müller glia in mammalian retina
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Photobiomodulation therapy has been proposed as a promisingtherapeutic approach for retinal degenerative diseases. However,its effect on the regenerative capacity in mammalian retina andits intracellular signalling mechanisms remain unknown. Here,we show that photobiomodulation with 670 nm light stimulatesMüller glia cell cycle re-entry and dedifferentiation into aprogenitor-like state in both the uninjured and injured retina. We also find that 670 nm light treatment inhibits the Hippopathway, which is activated in Müller glia following NaIO3-induced retinal injury. YAP, a major downstream effector ofthe Hippo signalling pathway was translocated into the nucleusof Müller glia along with YAP dephosphorylation in retinatreated with 670 nm light. Deficiency of YAP attenuated Müllerglia cell cycle re-entry and dedifferentiation. Our data revealthat the Hippo-YAP signalling pathway is associated with thephotostimulatory effect on regenerative response in mammalianretina, and suggest a potential therapeutic strategy for retinaldegenerative diseases.
Abstract
Photobiomodulation therapy has been proposed as a promisingtherapeutic approach for retinal degenerative diseases. However,its effect on the regenerative capacity in mammalian retina andits intracellular signalling mechanisms remain unknown. Here,we show that photobiomodulation with 670 nm light stimulatesMüller glia cell cycle re-entry and dedifferentiation into aprogenitor-like state in both the uninjured and injured retina. We also find that 670 nm light treatment inhibits the Hippopathway, which is activated in Müller glia following NaIO3-induced retinal injury. YAP, a major downstream effector ofthe Hippo signalling pathway was translocated into the nucleusof Müller glia along with YAP dephosphorylation in retinatreated with 670 nm light. Deficiency of YAP attenuated Müllerglia cell cycle re-entry and dedifferentiation. Our data revealthat the Hippo-YAP signalling pathway is associated with thephotostimulatory effect on regenerative response in mammalianretina, and suggest a potential therapeutic strategy for retinaldegenerative diseases.
참고문헌 (23)
- Bernardos RL, "Late-stage neuronal progenitors in the retina are radial Müller glia that function as retinal stem cells", J Neurosci, 27, pp.7028~7040 (2007)
- Raymond PA, "How the neural retina regenerates", Results Probl Cell Differ, 31, pp.197~218 (2000)
- Dyer MA, "Control of Müller glial cell proliferation and activation following retinal injury", Nat Neurosci, 3, pp.873~880 (2000)
- Karl MO, "Stimulation of neural regeneration in the mouse retina", Proc Natl Acad Sci USA, 105, pp.19508~19513 (2008)
- Camargo FD, "YAP1increases organ size and expands undifferentiated progenitor cells", Curr Biol, 17, pp.2054~2060 (2007)
- Huang J, "The Hippo signaling pathway coordinately regulates cell proliferation and apoptosis by inactivating Yorkie, the Drosophila homolog of YAP", Cell, 122, pp.421~434 (2005)
- Zhao B, "TEAD mediates YAPdependent gene induction and growth control", Genes Dev, 22, pp.1962~1971 (2008)
- Udan RS, "Hippo promotes proliferation arrest and apoptosis in the Salvador", Warts pathway, 2003, pp.5, 914~920
- Moya IM, "Hippo-YAP", TAZ signalling in organ regeneration and regenerative medicine, 2019, pp.20, 211~226
- Rueda EM, "The Hippo pathway blocks mammalian retinal Müller glial cell reprogramming", Cell Rep, 27, pp.1637~1649 (2019)
- Albarracin R, "Photobiomodulation protects the retina from light-induced photoreceptor degeneration", Invest Ophthalmol Vis Sci, 52, pp.3582~ (2011)
- Geneva II, "Photobiomodulation for the treatment of retinal diseases : a review", Int J Ophthalmol, 9, pp.145~152 (2016)
- Albarracin R, "670 nm light mitigates oxygen-induced degeneration in C57BL", 6J mouse retina, 2013, pp.14, 125~
- Hamblin MR, "Mechanisms and applications of the anti-inflammatory effects of photobiomodulation", AIMS Biophysics, 4, pp.337~361 (2017)
- Gkotsi D, "Recharging mitochondrial batteries in old eyes. Near infra-red increases ATP", Exp Eye Res, 122, pp.50~53 (2014)
- Begum R, "Treatment with 670 nm light up regulates cytochrome C oxidase expression and reduces inflammation in an age-related macular degeneration model", PLoS One, 8, pp.e57828~ (2013)
- Hamblin MR, "Mechanisms and mitochondrial redox signaling in photobiomodulation", Photochem Photobiol, 94, pp.199~212 (2018)
- Calaza KC, "Mitochondrial decline precedes phenotype development in the complement factor H mouse model of retinal degeneration but can be corrected by near infrared light", Neurobiol Aging, 36, pp.2869~2876 (2015)
- Chowers G, "Course of sodium iodate-induced retinal degeneration in albino and pigmented mice", Invest Ophthalmol Vis Sci, 58, pp.2239~ (2017)
- DeRan M, "Energy stress regulates Hippo-YAP signaling involving AMPK-mediated regulation of angiomotin-like 1 protein", Cell Rep, 9, pp.495~503 (2014)
- Ji F, "UCP2 regulates embryonic neurogenesis via ROS-mediated Yap alternation in the developing neocortex", Stem Cells, 35, pp.1479~1492 (2017)
- Hamon A, "Linking YAP to Müller glia quiescence exit in the degenerative retina", Cell Rep, 27, pp.1712~1725 (2019)
- Yao K, "Wnt regulates proliferation and neurogenic potential of Müller glial cells via a Lin28", let-7 miRNA-dependent pathway in adult mammalian retinas, 2016, pp.17, 165~178