Prmt7 is required for the osteogenic differentiation of mesenchymal stem cells via modulation of BMP signaling
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Arginine methylation, which is catalyzed by protein argininemethyltransferases (Prmts), is known to play a key role in variousbiological processes. However, the function of Prmts inosteogenic differentiation of mesenchymal stem cells (MSCs)has not been clearly understood. In the current study, weattempted to elucidate a positive role of Prmt7 in osteogenicdifferentiation. Prmt7-depleted C3H/10T1/2 cells or bone marrowmesenchymal stem cells (BMSCs) showed the attenuatedexpression of osteogenic specific genes and Alizarin red stainingcompared to the wild-type cells. Furthermore, we foundthat Prmt7 deficiency reduced the activation of bone morphogeneticprotein (BMP) signaling cascade, which is essential forthe regulation of cell fate commitment and osteogenesis. Takentogether, our data indicate that Prmt7 plays important regulatoryroles in osteogenic differentiation.
Abstract
Arginine methylation, which is catalyzed by protein argininemethyltransferases (Prmts), is known to play a key role in variousbiological processes. However, the function of Prmts inosteogenic differentiation of mesenchymal stem cells (MSCs)has not been clearly understood. In the current study, weattempted to elucidate a positive role of Prmt7 in osteogenicdifferentiation. Prmt7-depleted C3H/10T1/2 cells or bone marrowmesenchymal stem cells (BMSCs) showed the attenuatedexpression of osteogenic specific genes and Alizarin red stainingcompared to the wild-type cells. Furthermore, we foundthat Prmt7 deficiency reduced the activation of bone morphogeneticprotein (BMP) signaling cascade, which is essential forthe regulation of cell fate commitment and osteogenesis. Takentogether, our data indicate that Prmt7 plays important regulatoryroles in osteogenic differentiation.
참고문헌 (28)
- Salhotra A, "Mechanisms of bone development and repair", Nat Rev Mol Cell Biol, 21, pp.696~711 (2020)
- Chen G, "TGF-beta and BMP signaling in osteoblast differentiation and bone formation", Int J Biol Sci, 8, pp.272~288 (2012)
- Li RD, "Biphasic effects of TGFbeta1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells", BMB Rep, 45, pp.509~514 (2012)
- Chae YM, "Upregulation of smpd3 via BMP2 stimulation and Runx2", BMB Rep, 42, pp.86~90 (2009)
- Wolf SS, "The protein arginine methyltransferase family : an update about function, new perspectives and the physiological role in humans", Cell Mol Life Sci, 66, pp.2109~2121 (2009)
- Jain K, "PRMT7 as a unique member of the protein arginine methyltransferase family : a review", Arch Biochem Biophys, 665, pp.36~45 (2019)
- Agolini E, "Expanding the clinical and molecular spectrum of PRMT7 mutations : 3 additional patients and review", Clin Genet, 93, pp.675~681 (2018)
- Ahn BY, "PRMT7 ablation in cardiomyocytes causes cardiac hypertrophy and fibrosis through beta-catenin dysregulation", Cell Mol Life Sci, 79, pp.99~ (2022)
- Jeong HJ, "Prmt7 promotes myoblast differentiation via methylation of p38MAPK on arginine residue 70", Cell Death Differ, 27, pp.573~586 (2020)
- Ye H, "PRMT1-mediated arginine methylation promotes postnatal calvaria bone formation through BMP-Smad signaling", Bone, 176, pp.116887~ (2023)
- Kota SK, "PRMT5 inhibition promotes osteogenic differentiation of mesenchymal stromal cells and represses basal interferon stimulated gene expression", Bone, 117, pp.37~46 (2018)
- Miron RJ, "Osteoinduction : a review of old concepts with new standards", J Dent Res, 91, pp.736~744 (2012)
- Xu J, "Arginine Methylation Initiates BMP-Induced Smad Signaling", Mol Cell, 51, pp.5~19 (2013)
- Katsuno Y, "Arginine methylation of SMAD7 by PRMT1 in TGF-beta-induced epithelial-mesenchymal transition and epithelial stem-cell generation", J Biol Chem, 293, pp.13059~13072 (2018)
- da Silva Meirelles L, "Mesenchymal stem cells reside in virtually all post-natal organs and tissues", J Cell Sci, 119, pp.2204~2213 (2006)
- Jeon MJ, "Activation of peroxisome proliferator-activated receptor-gamma inhibits the Runx2-mediated transcription of osteocalcin in osteoblasts", J Biol Chem, 278, pp.23270~23277 (2003)
- Zhang YY, "Down-regulation of type I Runx2 mediated by dexamethasone is required for 3T3-L1 adipogenesis", Mol Endocrinol, 26, pp.798~808 (2012)
- Justesen J, "Adipocyte tissue volume in bone marrow is increased with aging and in patients with osteoporosis", Biogerontology, 2, pp.165~171 (2001)
- Kang H, "The role of microRNAs in cell fate determination of mesenchymal stem cells : balancing adipogenesis and osteogenesis", BMB Rep, 48, pp.319~323 (2015)
- Jeong HJ, "Prmt7 deficiency causes reduced skeletal muscle oxidative metabolism and age-related obesity", Diabetes, 65, pp.1868~1882 (2016)
- Leem YE, "PRMT7deficiency enhances adipogenesis through modulation of C", EBP-beta, 2019, pp.517, 484~490
- Guasto A, "Signaling pathways in bone development and their related skeletal dysplasia", Int J Mol Sci, 22, pp.4321~ (2021)
- Halloran D, "Bone morphogenetic protein-2 in development and bone homeostasis", J Dev Biol, 8, pp.19~ (2020)
- Jonason JH, "Post-translational regulation of Runx2 in bone and cartilage", J Dent Res, 88, pp.693~703 (2009)
- Mishina Y, "Bone morphogenetic protein type IA receptor signaling regulates postnatal osteoblast function and bone remodeling", J Biol Chem, 279, pp.27560~27566 (2004)
- Shen H, "BMP12 induces tenogenic differentiation of adipose-derived stromal cells", PLoS One, 8, pp.e77613~ (2013)
- Inamitsu M, "Methylation of Smad6 by protein arginine N-methyltransferase 1", FEBS Lett, 580, pp.6603~6611 (2006)
- Soleimani M, "A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow", Nat Protoc, 4, pp.102~106 (2009)