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Collagenase와 esterase가 상아질 접착강도와 nanoleakage에 미치는 영향

Effects of collagenase and esterase on dentin bond strength and nanoleakage

이상훈|김종철|김정욱|김영재|장기택|한세현|정영정
대한소아치과학회 (The Korean Academy Of Pediatric Dentistry)|2008.08|대한소아치과학회지 (Journal of the Korean academy of pediatric dentistry)|제35권 제3호|pp. 389-398

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참고문헌

키워드

CollagenaseBond strengthEsterase접착강도극미세누출Nanoleakage
주제분류치의학

초록

본 연구는 상아질 접착계면에서 collagenase와 esterase가 접착강도와 극미세누출에 미치는 영향을 살펴보기 위해 시행하였다. 발치된 치아의 교합면 상아질에 Single Bond 2(SB)와 Clearfil SE bond(SE)를 사용하여 상아질-레진 접착시편을 제작하고, 시편을 인산완충용액(PBS)에 24시간(I), 또는 PBS(II), collagenase 용액(III), esterase 용액(IV)에 4주간 보관한 후 질산은 용액으로 염색하였다. 시편의 미세인장접착강도(μTBS)와 질산은 침투 면적을 측정하여 다음과 같은 결과를 얻었다. 1. SB군의 접착강도가 II ~ IV군에서 SE군에 비해 낮은 값을 나타내었다(p<0.05). SB군은 II ~ IV군의 접착강도가 I군 에 비해 낮은 값을 보였으나(p<0.05), SE군의 접착강도는 I ~ IV군간에 차이를 보이지 않았다(p>0.05). 2. SB군의 질산은 침투 면적이 SE군에 비해 높았으며(p<0.05), SB군과 SE군에서 질산은 침투 면적은 I ~ IV군간에 차 이를 보이지 않았다(p>0.05). 3. 접착강도와 질산은 침투 면적은 SE군의 I, II, III군에서 음의 상관관계를 보였다(p<0.05).

Abstract

The purpose of this study was to evaluate the effects of collagenase and esterase on dentin bond strength and nanoleakage. Resin composites were bonded to occlusal dentin of premolars with Single Bond 2(SB) and Clearfil SE Bond(SE). After the microtensile specimens were prepared and stored in PBS for 24 hours(I) or, PBS(II), collagenase(III), esterase(IV) solution for 4 weeks, the specimens were stained with silver nitrate solution. Microtensile bond strength(μTBS) and silver penetration area were measured and, the results were as follows: 1. For group II, III, and IV, the bond strengths of SB were lower than those of SB(p<0.05). The bond strengths of SB II, III, and IV were lower than that of SB I(p<0.05). There was no difference among the bond strengths of SE I ~ IV groups(p>0.05). 2. Silver penetration areas of SB were higher than those of SE for all storage groups(p<0.05). In SB and SE groups, there was no significant difference of silver penetration area among I ~ IV groups(p>0.05). 3. SE I, II, and III showed inverse relationship between the bond strengths and the silver penetration areas(p<0.05).

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