In Vivo and Ex Vivo Skin Reactions after Multiple Pulses of 1,064-nm, Microlens Array-type, Picosecond Laser Treatment
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Background and Objectives A picosecond-domain laser treatment using a microlens array (MLA) or a diffractive optical element elicits therapeutic micro-injury zones in the skin. This study examined the patterns of tissue reactions after delivering multiple pulses of 1,064-nm, MLA-type, picosecond neodymium:yttriumaluminum- garnet laser treatment. Materials and Methods Multiple pulses of picosecond laser treatment were delivered to ex vivo human or brown micropig skin and analyzed histopathologically. A highspeed cinematographic study was performed to visualize the multiple pulses of picosecond laser energy-induced skin reactions in in vivo human skin. Results In the ex vivo human skin, a picosecond laser treatment at a fluence of 0.3 J/cm2 over 100 non-stacking passes generated multiple lesions of thermally-initiated laser-induced optical breakdown (TI-LIOB) in the epidermis and dermis. In the ex vivo micropig skin, stacking pulses of 20, 40, 60, 80, and 100 at a fluence of 0.3 J/cm2 generated distinct round to oval zones of tissue coagulation in the mid to lower dermis. High-speed cinematography captured various patterns of twinkling, micro-spot reactions on the skin surface over 100 stacked pulses of a picosecond laser treatment. Conclusion Multiple pulses of 1,064-nm, MLA-type, picosecond laser treatment elicit marked TI-LIOB reactions in the epidermis and areas of round to oval thermal coagulation in the mid to deep dermis.
Abstract
Background and Objectives A picosecond-domain laser treatment using a microlens array (MLA) or a diffractive optical element elicits therapeutic micro-injury zones in the skin. This study examined the patterns of tissue reactions after delivering multiple pulses of 1,064-nm, MLA-type, picosecond neodymium:yttriumaluminum- garnet laser treatment. Materials and Methods Multiple pulses of picosecond laser treatment were delivered to ex vivo human or brown micropig skin and analyzed histopathologically. A highspeed cinematographic study was performed to visualize the multiple pulses of picosecond laser energy-induced skin reactions in in vivo human skin. Results In the ex vivo human skin, a picosecond laser treatment at a fluence of 0.3 J/cm2 over 100 non-stacking passes generated multiple lesions of thermally-initiated laser-induced optical breakdown (TI-LIOB) in the epidermis and dermis. In the ex vivo micropig skin, stacking pulses of 20, 40, 60, 80, and 100 at a fluence of 0.3 J/cm2 generated distinct round to oval zones of tissue coagulation in the mid to lower dermis. High-speed cinematography captured various patterns of twinkling, micro-spot reactions on the skin surface over 100 stacked pulses of a picosecond laser treatment. Conclusion Multiple pulses of 1,064-nm, MLA-type, picosecond laser treatment elicit marked TI-LIOB reactions in the epidermis and areas of round to oval thermal coagulation in the mid to deep dermis.
참고문헌 (15)
- Balu M, "In vivo multiphoton-microscopy of picosecond-laserinduced optical breakdown in human skin", Lasers Surg Med, 49, pp.555~562 (2017)
- Tanghetti EA, "The histology of skin treated with a picosecond alexandrite laser and a fractional lens array", Lasers Surg Med, 48, pp.646~652 (2016)
- Habbema L, "Minimally invasive non-thermal laser technology using laser-induced optical breakdown for skin rejuvenation", J Biophotonics, 5, pp.194~199 (2012)
- Brauer JA, "Use of a picosecond pulse duration laser with specialized optic for treatment of facial acne scarring", JAMA Dermatol, 151, pp.278~284 (2015)
- Bernstein EF, "Treatment of acne scarring with a novel fractionated, dual-wavelength, picosecond-domain laser incorporating a novel holographic beam-splitter", Lasers Surg Med, 49, pp.796~802 (2017)
- Varghese B, "Influence of absorption induced thermal initiation pathway on irradiance threshold for laser induced breakdown", Biomed Opt Express, 6, pp.1234~1240 (2015)
- Tanghetti Md E, "A comparative study with a 755nm picosecond Alexandrite laser with a diffractive lens array and a 532nm", 1064nm Nd : YAG with a holographic optic, 2018, pp.50, 37~44
- Lee HC, "Pattern analysis of 532-and 1, 064-nm picosecond-domain laserinduced immediate tissue reactions in ex vivo pigmented micropig skin", Sci Rep, 9, pp.4186~ (2019)
- Chung HJ, "Pattern analysis of 532-and 1064-nm microlens array-type, picosecond-domain laser-induced tissue reactions in ex vivo human skin", Lasers Med Sci, 34, pp.1207~1215 (2019)
- Lee HC, "High-energy, sub-nanosecond linearly polarized passively Q-switched MOPA laser system", Opt Laser Technol, 95, pp.81~85 (2017)
- Uzunbajakava NE, "Highlighting the nuances behind interaction of picosecond pulses with human skin : relating distinct laser-tissue interactions to their potential in cutaneous interventions", Proc SPIE, 10492, pp.1049206~ (2018)
- Guida S, "Picosecond laser treatment of atrophic and hypertrophic surgical scars : In vivo monitoring of results by means of 3D imaging and reflectance confocal microscopy", Skin Res Technol, 25, pp.896~902 (2019)
- Kim HK, "Interactive tissue reactions of 1064-nm focused picosecond-domain laser and dermal cohesive polydensified matrix hyaluronic acid treatment in in vivo rat skin", Skin Res Technol, 26, pp.683~689 (2020)
- Kim JE, "Picosecond-domain fractional laser treatment over hyaluronic acid fillers: in vivo and clinical studies", Lasers Surg Med (2020)
- Na J, "Electromagnetic initiation and propagation of bipolar radiofrequency tissue reactions via invasive non-insulated microneedle electrodes", Sci Rep, 5, pp.16735~ (2015)