The Effectiveness of 448-kHz Capacitive Resistive Monopolar Radiofrequency for Subcutaneous Fat Reduction in a Porcine Model
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Background and Objectives The effectiveness of many physiotherapy modalities in reducing subcutaneous fat has been investigated in numerous previous studies. However, to the best of our knowledge, there have been no attempts to determine the effectiveness of physiotherapy modalities in body contouring. The present report determined the effect of 448-kHz capacitive resistive monopolar radiofrequency (CRMRF) in a porcine model. Materials and Methods This study investigated the effect of selective destruction of the subcutaneous fat layer in abdominal fat tissue using CRMRF. The effects of two types of CRMRF (capacitive electric transfer (CET) and resistive electric transfer (RET)) treatment were evaluated using regular digital photography in addition to thermal imaging evaluation, ultrasound measurement, hematological evaluation, and histologic analyses (H&E (hematoxylin and eosin), Oil red O, and immunohistochemistry staining). Results Preclinical evaluation was performed to obtain the data for comparison of the safety and efficacy of the subcutaneous fat reduction after applying CRMRF using CET and RET. After treatment, the thermal transmission was effective, and a 42-47°C temperature change was observed in the fat layer while an approximately temperature of 42°C was confirmed on the skin surface. Moreover, after the application of both types of CRMRF treatment, fibrotic septa were observed in the adipose tissue induced by heat at the treatment sites. TUNEL staining was also performed to confirm the process of apoptosis in the adipocytes. Conclusion These results suggest that both CET and RET for CRMRF treatment are safe and effective for subcutaneous fat reduction in a porcine model.
Abstract
Background and Objectives The effectiveness of many physiotherapy modalities in reducing subcutaneous fat has been investigated in numerous previous studies. However, to the best of our knowledge, there have been no attempts to determine the effectiveness of physiotherapy modalities in body contouring. The present report determined the effect of 448-kHz capacitive resistive monopolar radiofrequency (CRMRF) in a porcine model. Materials and Methods This study investigated the effect of selective destruction of the subcutaneous fat layer in abdominal fat tissue using CRMRF. The effects of two types of CRMRF (capacitive electric transfer (CET) and resistive electric transfer (RET)) treatment were evaluated using regular digital photography in addition to thermal imaging evaluation, ultrasound measurement, hematological evaluation, and histologic analyses (H&E (hematoxylin and eosin), Oil red O, and immunohistochemistry staining). Results Preclinical evaluation was performed to obtain the data for comparison of the safety and efficacy of the subcutaneous fat reduction after applying CRMRF using CET and RET. After treatment, the thermal transmission was effective, and a 42-47°C temperature change was observed in the fat layer while an approximately temperature of 42°C was confirmed on the skin surface. Moreover, after the application of both types of CRMRF treatment, fibrotic septa were observed in the adipose tissue induced by heat at the treatment sites. TUNEL staining was also performed to confirm the process of apoptosis in the adipocytes. Conclusion These results suggest that both CET and RET for CRMRF treatment are safe and effective for subcutaneous fat reduction in a porcine model.
참고문헌 (23)
- Seidell JC, "Obesity and fat distribution in relation to health--current insights and recommendations", World Rev Nutr Diet, 50, pp.57~91 (1987)
- Pasquali R, "Effect of obesity and body fat distribution on sex hormones and insulin in men", Metabolism, 40, pp.101~104 (1991)
- Caterson ID, "Obesity: epidemiology and possible prevention", Best Pract Res Clin Endocrinol Metab, 16, pp.595~610 (2002)
- Kennedy J, "Noninvasive subcutaneous fat reduction: a review", J Eur Acad Dermatol Venereol, 29, pp.1679~1688 (2015)
- Sasaki GH, "Noninvasive selective cryolipolysis and reperfusion recovery for localized natural fat reduction and contouring", Aesthet Surg J, 34, pp.420~431 (2014)
- Guth F, "Immediate effect and safety of HIFU single treatment for male subcutaneous fat reduction", J Cosmet Dermatol, 17, pp.385~389 (2018)
- Downie J, "Contactless abdominal fat reduction with selective RF™ evaluated by Magnetic Resonance Imaging (MRI):case study", J Drugs Dermatol, 15, pp.491~495 (2016)
- Caruso-Davis MK, "Efficacy of low-level laser therapy for body contouring and spot fat reduction", Obes Surg, 21, pp.722~729 (2011)
- Gorgu M, "Effects of device variables to radiofrequency (RF) applications", J Cosmet Laser Ther, 21, pp.364~371 (2019)
- Black DR, "Radiofrequency (RF) effects on blood cells, cardiac, endocrine, and immunological functions", Bioelectromagnetics, (Suppl 6), pp.S187~S195 (2003)
- Hong K, "Radiofrequency ablation: mechanism of action and devices", J Vasc Interv Radiol, 21(8Suppl), pp.S179~S186 (2010)
- Helfrich YR, "Overview of skin aging and photoaging", Dermatol Nurs, 20, pp.177~183 (2008)
- Boisnic S, "A clinical and biological evaluation of a novel, noninvasive radiofrequency device for the long-term reduction of adipose tissue", Lasers Surg Med, 46, pp.94~103 (2014)
- Dunbar SW, "Radiofrequency in cosmetic dermatology:an update", J Drugs Dermatol, 14, pp.1229~1238 (2015)
- Park YJ, "Radiofrequency volume reduction of gastrocnemius muscle hypertrophy for cosmetic purposes", Aesthetic Plast Surg, 31, pp.53~61 (2007)
- Lolis MS, "Radiofrequency in cosmetic dermatology:a review", Dermatol Surg, 38, pp.1765~1776 (2012)
- Sadick NS, "A prospective clinical study to evaluate the efficacy and safety of cellulite treatment using the combination of optical and RF energies for subcutaneous tissue heating", J Cosmet Laser Ther, 6, pp.187~190 (2004)
- Spottorno J, "Influence of electrodes on the 448 kHz electric currents created by radiofrequency: a finite element study", Electromagn Biol Med, 36, pp.306~314 (2017)
- Hernández-Bule ML, "Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells", Mol Med Rep, 13, pp.3895~3903 (2016)
- Hernández-Bule ML, "Electric stimulation at 448 kHz promotes proliferation of human mesenchymal stem cells", Cell Physiol Biochem, 34, pp.1741~1755 (2014)
- Kwon TR, "Improved methods for evaluating pre-clinical and histological effects of subcutaneous fat reduction using high-intensity focused ultrasound in a porcine model", Skin Res Technol, 23, pp.194~201 (2017)
- Kumaran B, "Treatment using 448kHz capacitive resistive monopolar radiofrequency improves pain and function in patients with osteoarthritis of the knee joint: a randomised controlled trial", Physiotherapy, 105, pp.98~107 (2019)
- Portt L, "Anti-apoptosis and cell survival: a review", Biochim Biophys Acta, 1813, pp.238~259 (2011)