The Effect of an Optical Clearing Agent on Tissue Prior to 1064-nm Laser Therapy
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Background and Objectives Although lasers have been widely applied in tissue treatment, the light penetration depth in tissues is limited by the tissue turbidity and affected by its absorption and scattering characteristics. This study investigated the effect of using an optical clearing agent (OCA) on tissue to improve the therapeutic effect of 1064 nm wavelength laser light by reducing the heat generated on the skin surface and increasing the penetration depth. Materials and Methods A diode laser (λ = 1064 nm) was applied to a porcine specimen with and without OCA to investigate the penetration depth of the laser light and temperature distribution. A numerical simulation using the finite element method was performed to investigate the temperature distribution of the specimen compared to ex-vivo experiments using a thermocouple and double-integrating sphere to measure the temperature profile and optical properties of the tissue, respectively. Results Simulation results showed a decrease in tissue surface temperature with increased penetration depth when the OCA was applied. Furthermore, both absorption and scattering coefficients decreased with the application of OCA. In ex-vivo experiments, temperatures decreased for the tissue surface and the fat layer with the OCA, but not for the muscle layer. Conclusion The use of an OCA may be helpful for reducing surface heat generation and enhance the light penetration depth in various near-infrared laser treatments.
Abstract
Background and Objectives Although lasers have been widely applied in tissue treatment, the light penetration depth in tissues is limited by the tissue turbidity and affected by its absorption and scattering characteristics. This study investigated the effect of using an optical clearing agent (OCA) on tissue to improve the therapeutic effect of 1064 nm wavelength laser light by reducing the heat generated on the skin surface and increasing the penetration depth. Materials and Methods A diode laser (λ = 1064 nm) was applied to a porcine specimen with and without OCA to investigate the penetration depth of the laser light and temperature distribution. A numerical simulation using the finite element method was performed to investigate the temperature distribution of the specimen compared to ex-vivo experiments using a thermocouple and double-integrating sphere to measure the temperature profile and optical properties of the tissue, respectively. Results Simulation results showed a decrease in tissue surface temperature with increased penetration depth when the OCA was applied. Furthermore, both absorption and scattering coefficients decreased with the application of OCA. In ex-vivo experiments, temperatures decreased for the tissue surface and the fat layer with the OCA, but not for the muscle layer. Conclusion The use of an OCA may be helpful for reducing surface heat generation and enhance the light penetration depth in various near-infrared laser treatments.
참고문헌 (20)
- Rastegar S, "Theoretical analysis of equivalency of high-power diode laser(810 nm)and Nd : YAG laser(1064 nm)for coagulation of tissue : predictions for prostate coagulation", Proc SPIE, 1646, pp.150~160 (1992)
- Alayat MS, "Long-term effect of high-intensity laser therapy in the treatment of patients with chronic low back pain : a randomized blinded placebo-controlled trial", Lasers Med Sci, 29, pp.1065~1073 (2014)
- Lee S, "Development of the theragnostic optical system for a high-intensity laser therapy(HILT)", Lasers Med Sci, 29, pp.1585~1591 (2014)
- Alayat MSM, "Efficacy of pulsed Nd : YAG laser in the treatment of patients with knee osteoarthritis : a randomized controlled trial", Lasers Med Sci, 32, pp.503~511 (2017)
- Taylor DL, "Comparison of a high power diode laser with the Nd : YAG laser using in situ wound strength analysis of healing cutaneous incisions", Lasers Surg Med, 21, pp.248~254 (1997)
- Bordera GB, "Use of high-power diode laser at 1060nm for the treatment of vascular lesions", J Vasc Surg Cases Innov Tech, 5, pp.415~418 (2019)
- Yeh AT, "Molecular interactions of exogenous chemical agents with collagen--implications for tissue optical clearing", J Biomed Opt, 11, pp.014003~ (2006)
- Rylander CG, "Dehydration mechanism of optical clearing in tissue", J Biomed Opt, 11, pp.041117~ (2006)
- Genina EA, "Optical clearing of human skin : comparative study of permeability and dehydration of intact and photothermally perforated skin", J Biomed Opt, 13, pp.021102~ (2008)
- Reynoso FJ, "Implementation of a multisource model for gold nanoparticle-mediated plasmonic heating with near-infrared laser by the finite element method", Med Phys, 40, pp.073301~ (2013)
- 신성선, "레이저의 피부 흡수에 대한 시뮬레이션", 공학기술논문지, 8(1), pp.59~64 (2015), 공학기술연구원[DOI]
- Moulton K, "Use of glycerol as an optical clearing agent for enhancing photonic transference and detection of Salmonella typhimurium through porcine skin", J Biomed Opt, 11, pp.054027~ (2006)
- Salomatina E, "Optical properties of normal and cancerous human skin in the visible and near-infrared spectral range", J Biomed Opt, 11, pp.064026~ (2006)
- Pickering JW, "Double-integrating-sphere system for measuring the optical properties of tissue", Appl Opt, 32, pp.399~410 (1993)
- Bäumler W, "The effects of intense pulsed light(IPL)on blood vessels investigated by mathematical modeling", Lasers Surg Med, 39, pp.132~139 (2007)
- Ataie-Fashtami L, "Simulation of heat distribution and thermal damage patterns of diode hair-removal lasers : an applicable method for optimizing treatment parameters", Photomed Laser Surg, 29, pp.509~515 (2011)
- Duck FA, "Physical properties of tissue : a comprehensive reference book", pp.10~42 (1990), Academic Press
- Genina EA, "Rapid ultrasound optical clearing of human light and dark skin", IEEE Trans Med Imaging, 39, pp.3198~3206 (2020)
- Gabay I, "Increasing the penetration depth for ultrafast laser tissue ablation using glycerol based optical clearing", Proc SPIE, 9707, pp.1~8 (2016)
- Nachabé R, "Estimation of lipid and water concentrations in scattering media with diffuse optical spectroscopy from 900 to 1, 600 nm", J Biomed Opt, 15, pp.037015~ (2010)