정중 과잉치 법랑질의 SEM/EDS 분석
SEM/EDS Analysis of the Enamel in Mesiodens
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이 연구는 정중 과잉치 법랑질의 구조학적 및 형태학적 특징에 대하여 주사형 전자현미경(FE-SEM)과 에너지 분산형 분광분석법(EDS)을 이용하여 관찰하고 영구 중절치와 유중절치의 법랑질 구조 및 형태와 차이점에 대하여 평가하고자 하였다. 15개의 정중 과잉치, 영구 중절치와 유중절치를 이용하였으며, 법랑질의 두께와 법랑소주 직경에 대하여 FE-SEM을 이용하여 측정및 관찰하고, 성분 분석을 위하여 EDS를 이용하였다. 그 결과 법랑소주 두께의 경우 영구 중절치, 정중 과잉치, 유중절치 순으로 나타났다. 법랑소주의 직경의 경우 영구중절치와 정중 과잉치에서는 유의할 만한 차이가 없었으며, 유중절치는 두 그룹에 비해서 작게 나타났다. 성분 분석 시 칼슘의 경우 영구 중절치와 정중 과잉치에 있어서 통계적 유의성이 없었으며, 유중절치는 작게 나타났다. Ca/P ratio의 경우 정중 과잉치와 영구 중절치는 비슷한 수치를 나타내며 통계적 유의성이 없었고, 유중절치보다는 두 그룹 모두 높게 나타났다. Ca/C ratio의 경우 마찬가지로 정중 과잉치와 영구 중절치에서 유중절치보다 높게 나타났다. 이를 통하여 정중 과잉치의 법랑질특성은 유중절치보다는 영구 중절치와 더 유사함을 알 수 있고, 임상적으로 정중 과잉치의 활용 시 영구 중절치와 비슷한 법랑질 성질을 지님을 유추할 수 있다.
Abstract
The aim of this study was to evaluate and compare the microstructural properties and mineralization quality of mesiodens with permanent and primary central incisors. Fifteen mesiodens, permanent and primary central incisors were collected. The enamel rod diameter and enamel thickness were observed and measured using field emission scanning electron microscope (FE-SEM). Chemical composition of the enamel was analyzed using energy dispersive X-ray spectrometer (EDS). The measurements were then assessed using the one-way ANOVA and Tukey test. There was no statistically significant difference in the enamel rod diameter between mesiodens and permanent central incisors. However, enamel rods of primary central incisors were smaller than remaining two groups. The thickness of enamel was thick in order of permanent central incisors, mesiodens, primary central incisors. In the composition analysis using EDS, there was no difference in the Ca/P ratio and Ca/C ratio between mesiodens and permanent central incisors, but small in primary central incisors. In conclusion, the microstructural properties of mesiodens were more similar to those of permanent central incisors compared to primary central incisors. Futher, the mineralization quality of mesiodens did not differ significantly from that of permanent central incisors.
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