본문 바로가기
KERD
논문KCI 등재

Effect of Polydeoxyribonucleotide on Human Periodontal Ligament Cells as a Storage Medium for Avulsed Tooth

이고은|김미선|최성철|채용권|남옥형|이효설|노상태
대한소아치과학회 (The Korean Academy Of Pediatric Dentistry)|2023.08|대한소아치과학회지 (Journal of the Korean academy of pediatric dentistry)|제50권 제3호|pp. 347-359

0

조회

0

다운로드

0

인용

0

참고문헌

키워드

Tooth avulsionStorage mediaPolydeoxyribonucleotide
주제분류치의학

초록

Objective: This study aimed to evaluate the suitability of polydeoxyribonucleotides (PDRN) as a storage medium for avulsed teeth. Materials and Methods: The viability of human periodontal ligament (PDL) cells stored in Hank’s balanced salt solution and PDRN solutions (concentrations, 10, 25, 50, and 100 μg/mL) and tap water was measured using the Cell Counting Kit-8 and Live/Dead assays. In addition, Nitric oxide detection and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to evaluate the anti-inflammatory effect of PDRN. Results: The viability of PDL cells stored in a 100 μg/mL PDRN solution was significantly higher than that of cells stored in the other solutions (p < 0.01). Furthermore, cells stored in 100 μg/mL PDRN solution demonstrated a significantly reduced NO production (p < 0.0001), and cells stored in 50 and 100 μg/mL PDRN solutions expressed significantly lower levels of tumor necrosis factor α, interleukin (IL) -4, IL-6, and IL-10 (p < 0.01) compared to cells stored in HBSS. Conclusion: The PDRN solution exhibited cell-preserving and anti-inflammatory effects on the PDL cells. The findings of this study can serve as a basis for further experiments directed at the development of an effective storage medium for avulsed teeth.

Abstract

Objective: This study aimed to evaluate the suitability of polydeoxyribonucleotides (PDRN) as a storage medium for avulsed teeth. Materials and Methods: The viability of human periodontal ligament (PDL) cells stored in Hank’s balanced salt solution and PDRN solutions (concentrations, 10, 25, 50, and 100 μg/mL) and tap water was measured using the Cell Counting Kit-8 and Live/Dead assays. In addition, Nitric oxide detection and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to evaluate the anti-inflammatory effect of PDRN. Results: The viability of PDL cells stored in a 100 μg/mL PDRN solution was significantly higher than that of cells stored in the other solutions (p < 0.01). Furthermore, cells stored in 100 μg/mL PDRN solution demonstrated a significantly reduced NO production (p < 0.0001), and cells stored in 50 and 100 μg/mL PDRN solutions expressed significantly lower levels of tumor necrosis factor α, interleukin (IL) -4, IL-6, and IL-10 (p < 0.01) compared to cells stored in HBSS. Conclusion: The PDRN solution exhibited cell-preserving and anti-inflammatory effects on the PDL cells. The findings of this study can serve as a basis for further experiments directed at the development of an effective storage medium for avulsed teeth.

참고문헌 (53)

  1. Andreason JO, "Textbook and Color Atlas of Traumatic Injuires to the Teeth" (2018), John Wiley & Sons
  2. Andersson L, "International Association of Dental Traumatology guidelines for the management of traumatic dental injuries: 2. Avulsion of permanent teeth", Dent Traumatol, 28, pp.88~96 (2012)
  3. Anderason J, "Replantation of 400 avulsed permanent incisors. 4. Factors related to periodontal ligament healing", Endod Dent Traumatol, 11, pp.76~89 (1995)
  4. Ahn HJ, "Expression of inflammatory cytokines and MMPs on replanted teeth at different extra-alveolar time : an ex vivo and in vivo study", Int J Pediatr Dent, 26, pp.301~309 (2016)
  5. Hiremath G, "Avulsion and storage media", J Investig Clin Dent, 2, pp.89~94 (2011)
  6. Subramaniam P, "Oral rehydration salt-liquid as a storage medium for avulsed tooth", Dent Traumatol, 31, pp.62~66 (2015)
  7. Siddiqui F, "Storage media for an avulsed tooth : nature to the rescue", Br J Med Health Res, 1, pp.1~10 (2014)
  8. Poi WR, "Storage media for avulsed teeth : a literature review", Braz Dent J, 24, pp.437~445 (2013)
  9. Rajakeerthi R, "Natural Product as the Storage medium for an avulsed tooth-A Systematic Review", Cumhur Dent J, 22, pp.249~256 (2019)
  10. Jain D, "Natural products as storage media for avulsed tooth", Saudi Endod J, 5, pp.107~ (2015)
  11. Hwang JY, "The use of green tea extract as a storage medium for the avulsed tooth", J Endod, 37, pp.962~967 (2011)
  12. Özan F, "Effect of propolis on survival of periodontal ligament cells : new storage media for avulsed teeth", J Endod, 33, pp.570~573 (2007)
  13. Nam OH, "Evaluation of delphinidin as a storage medium for avulsed teeth", BMC Oral Health, 23, pp.21~ (2023)
  14. Squadrito F, "Pharmacological activity and clinical use of PDRN", Front Pharmacol, 8, pp.224~ (2017)
  15. Guizzardi S, "Effects of heat deproteinate bone and polynucleotides on bone regeneration : an experimental study on rat", Micron, 38, pp.722~728 (2007)
  16. Pallio G, "Adenosine receptor stimulation by polydeoxyribonucleotide improves tissue repair and symptomology in experimental colitis", Front Pharmacol, 7, pp.273~ (2016)
  17. Jeong EK, "Protective effect of polydeoxyribonucleotide against renal ischemia-reperfusion injury in mice", Transplant Proc, 48, pp.1251~1257 (2016)
  18. Bizzoca D, "Polydeoxyribonucleotide in the Treatment of Tendon Disorders, from Basic Science to Clinical Practice : A Systematic Review", Int J Mol Sci, 24, pp.4582~ (2023)
  19. 양찬영, "김(Porphyra sp.)에서 추출한 PDRN (polydeoxyribonucleotide)의나일틸라피아(Oreochromis niloticus)에서의 상처 치유 효과", 한국어병학회지, 34(2), pp.233~241 (2021), 한국어병학회
  20. 송미희, "다양한 식물에서의 PDRN(Polydeoxyribonucleotide) 추출 수율 비교 및 상처치유 효능 분석", 한국정보전자통신기술학회 논문지, 15(5), pp.387~395 (2022), 한국정보전자통신기술학회
  21. Castellini C, "Anti inflammatory property of PDRN-an in vitro study on cultured macrophages", Adv Biosci Biotechnol, 8, pp.13~26 (2017)
  22. Bitto A, "Polydeoxyribonucleotide reduces cytokine production and the severity of collagen-induced arthritis by stimulation of adenosine A2A receptor", Arthritis Rheum, 63, pp.3364~3371 (2011)
  23. 이동섭, "The impact of polydeoxyribonucleotide on early bone formation in lateralwindow sinus floor elevation with simultaneous implant placement", Journal of Periodontal & Implant Science, 53(2), pp.157~169 (2023), 대한치주과학회[DOI]
  24. 김석곤, "Histologic study of bone-forming capacity on polydeoxyribonucleotide combined with demineralized dentin matrix", Maxillofacial Plastic Reconstructive Surgery, 38(2), pp.1~5 (2016), 대한악안면성형재건외과학회[DOI]
  25. 정준호, "Polydeoxyribonucleotide, as a Novel Approach for the Management of Medication-Related Osteonecrosis of the Jaw: A Preliminary Observational Study", Journal of Korean Dental Science, 11(2), pp.57~61 (2018), 대한치의학회
  26. 채용권, "Differential gene expression profiles of periodontal soft tissue from rat teeth after immediate and delayed replantation: a pilot study", Journal of Periodontal & Implant Science, 52(2), pp.127~140 (2022), 대한치주과학회[DOI]
  27. Müller DD, "Survival and complication analyses of avulsed and replanted permanent teeth", Sci Rep, 10, pp.2841~ (2020)
  28. Spinas E, "Delayed tooth replantation and inflammatory root resorption in childhood and adolescence", J Biol Regul Homeost Agents, 33, pp.623~627 (2019)
  29. Keum KY, "Effect of dexamethasone on root resorption after delayed replantation of rat tooth", J Endod, 29, pp.810~813 (2003)
  30. Maria T Colangelo, "The effects of polydeoxyribonucleotide on wound healing and tissue regeneration: a systematic review of the literature", Regenerative Medicine, 15(6), pp.1801~1821 (2020), Future Medicine Ltd[DOI]
  31. Shin DY, "Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing", Sci Rep, 10, pp.16811~ (2020)
  32. Kim TH, "Applications of Marine Organism-Derived Polydeoxyribonucleotide : Its Potential in Biomedical Engineering", Mar Drugs, 19, pp.296~ (2021)
  33. Colangelo MT, "Polydeoxyribonucleotide Regulation of Inflammation", Adv Wound Care, 9, pp.576~589 (2020)
  34. Ceravolo I, "Beneficial Effects of Polydeoxyribonucleotide(PDRN)in an In Vitro Model of Fuchs Endothelial Corneal Dystrophy", Pharmaceuticals, 15, pp.447~ (2022)
  35. Thellung S, "Polydeoxyribonucleotides enhance the proliferation of human skin fibroblasts : involvement of A2purinergic receptor subtypes", Life Sci, 64, pp.1661~1674 (1999)
  36. Han JH, "Anti-inflammatory effect of polydeoxyribonucleotide on zoledronic acid-pretreated and lipopolysaccharide-stimulated RAW 264. 7cells", Exp Ther Med, 16, pp.400~405 (2018)
  37. Matejka M, "Nitric oxide synthesis is increased in periodontal disease", J Periodontal Res, 33, pp.517~518 (1998)
  38. Lohinai Z, "Protective effects of mercaptoethylguanidine, a selective inhibitor of inducible nitric oxide synthase, in ligature-induced periodontitis in the rat", Br J Pharmacol, 123, pp.353~360 (1998)
  39. Lappin DF, "Inducible nitric oxide synthase expression in periodontitis", J Periodontal Res, 35, pp.369~373 (2000)
  40. Graves D, "Cytokines that promote periodontal tissue destruction", J Periodontol, 79(8 Suppl), pp.1585~1591 (2008)
  41. Takemura A, "Inhibitory effects of tumor necrosis factor-alpha on migration of human periodontal ligament cells", J Periodontol, 77, pp.883~890 (2006)
  42. Dana T. Graves, "Review of osteoimmunology and the host response in endodontic and periodontal lesions", Journal of Oral Microbiology, 3(1), pp.5304~ (2011), Informa UK Limited[DOI]
  43. 황재준, "Combination Therapy With Polydeoxyribonucleotide and Pirfenidone Alleviates Symptoms of Acute Respiratory Distress Syndrome in Human Lung Epithelial A549 Cells", International Neurourology Journal, 24, pp.56~64 (2020), 대한배뇨장애요실금학회[DOI]
  44. Murakani S, "Adenosine regulates the IL-1β-induced cellular functions of human gingival fibroblasts", Int Immunol, 13, pp.1533~1540 (2001)
  45. Liu Q, "Adenosine signaling mediates SUMO-1 modification of IκBαduring hypoxia and reoxygenation", J Biol Chem, 284, pp.13686~13695 (2009)
  46. Bitto A, "Adenosine receptor stimulation by polynucleotides(PDRN)reduces inflammation in experimental periodontitis", J Clin Periodontol, 40, pp.26~32 (2013)
  47. Altavilla D, "Polydeoxyribonucleotide(PDRN) : a safe approach to induce therapeutic angiogenesis in peripheral artery occlusive disease and in diabetic foot ulcers", Cardiovas Hematol Agents Medi Chem, 7, pp.313~321 (2009)
  48. Bitto A, "Polydeoxyribonucleotide(PDRN)restores blood flow in an experimental model of peripheral artery occlusive disease", J Vasc Surg, 48, pp.1292~1300 (2008)
  49. Galeano M, "Polydeoxyribonucleotide stimulates angiogenesis and wound healing in the genetically diabetic mouse", Wound Repair Regen, 16, pp.208~217 (2008)
  50. Polito F, "Polydeoxyribonucleotide restores blood flow in an experimental model of ischemic skin flaps", J Vasc Surg, 55, pp.479~488 (2012)
  51. Beak A, "Effect of polydeoxyribonucleotide on angiogenesis and wound healing in an in vitro model of osteoarthritis", Cell Transplant, 27, pp.1623~1633 (2018)
  52. Al-khafaji LK, "Effect of VEGF on the Success of Dental Tissue Regeneration in Delayed Replantation of Avulsed Teeth", Syst Rev Pharm, 11, pp.160~164 (2020)
  53. Mullane EM, "Effects of VEGF and FGF2 on the revascularization of severed human dental pulps", J Dent Res, 87, pp.1144~1148 (2008)

관련 논문AI추천

Storage Media for Avulsed Teeth: Current Evidence, Biological Principles, and Future Perspectives최성철대한소아치과학회 · 대한소아치과학회지2025 · 제52권 제4호Effect of (-)-epigallocatechin-3-gallate on maintaining the periodontal ligament cell viability of avulsed teeth: a preliminary study김창성, 윤정호, 정원균, 정임희, 최성호, Ah-Ran Cho대한치주과학회 · Journal of Periodontal & Implant Science2011 · 제41권 제1호수종의 저장용액에서의 치은섬유모세포 생존율의 비교연구이희수, 임유선한국치위생학회 · 한국치위생학회지2012 · 제12권 제4호저장용액의 온도에 따른 치주인대세포의 생존율김성오, 손흥규, 이제호, 조재현, 최병재, 최형준대한소아치과학회 · 대한소아치과학회지2007 · 제34권 제1호Evaluation of vitrification for cryopreservation of teeth김진, 이승종, 최성호, 최정민, Surangi C. Dissanayake대한치주과학회 · Journal of Periodontal & Implant Science2010 · 제40권 제3호탈락 유치 내 치수 세포의 보관 용액과 기간에 따른 생존김성오, 박지원, 손흥규, 송제선, 이제호, 전미정, 정한성대한소아치과학회 · 대한소아치과학회지2014 · 제41권 제1호

이 간행물의 다른 논문 (9건)

치과의사를 위한 노이즈 필터링 이어플러그의 청력 보호 효과에 관한 연구조다영, 이태양, 김익환, 정진세, 송제선, 박원서, 신승호pp. 239-251제퍼슨의 공감 척도를 사용한 치과 대학생의 공감 능력 측정김희민, 이대우, 양연미, 김재곤pp. 252-262유치의 치근단 방사선 사진에서 딥 러닝 알고리즘을 이용한 모델의 인접면 우식증 객체 탐지 능력의 평가전홍주, 김선미, 최남기pp. 263-276An in Vitro Study of the Effects of Different Dentin Bonding Agents on the Prevention of Tooth Discoloration and the Sealing Ability of Calcium Silicate-Based Cement in Regenerative Endodontic Procedures이제식, 김기민, 한지은pp. 277-291Analysis of Dental Antibiotic Prescriptions for Children and Adolescents in South Korea김지훈, 이승준pp. 292-306
원문 보기