탈락 유치 내 치수 세포의 보관 용액과 기간에 따른 생존
Effect of Storage Media and Duration on Pulpal Cell Viability in Exfoliated Deciduous Teeth
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초록
가정에서 유치가 탈락하였을 경우 쉽게 구할 수 있는 저장 용액에 보관한 후, 보관되었던 유치에서 세포를 배양할 수 있다면 지금보다 더 많은 유치로부터 줄기 세포를 추출할 수 있을 것이다. 본 연구는 유치의 치수 세포가 다양한 보관 용액의 종류와 기간에 영향을 받는지 확인해 보고자 하였다. 330개의 탈락 유치를 신선군, 건조군, 세포배양액 2, 4, 7일 보관군, 우유 2, 4, 7일 보관군, 생리식염수 2, 4, 7일 보관군으로 각각 30개씩 무작위로 나누었다. 각 군 간의 유치 치수 조직 세포의 일차 배양을 시행하여 성공한 개수와 실패한 개수를 계산하여 생존 성공률을 비교하였다. 일차 배양 결과 보관 기간이 늘어날수록 일차 배양 시 세포의 생존 성공률이 낮아지는 것으로 나타났다. 2일 보관군과 4일보관군까지 세포배양액, 우유, 생리식염수 간의 보관 용액에 따른 성공률의 유의할 만한 차이는 없었다. 그러나 7일 보관군에서는 세포배양액에 비해 우유와 생리식염수에 보관한 유치에서 성공률이 유의하게 떨어지는 것으로 나타났다. 유치 발거 후 우유나 생리식염수 등의 보관용액에 일정 기간 보관 후 그 치수 조직을 세포 배양에 사용하는 것이 가능하지만 보관 기간이 길어질수록 세포 획득 가능성이 줄어든다.
Abstract
If it is possible to preserve and culture cells from exfoliated deciduous teeth in a readily available storagemedium within each family, more stem cells would be obtained. This research is about the effect of storage mediaand time on pulpal cell viability of exfoliated deciduous teeth. 330 exfoliated deciduous teeth were randomly divided into 11 groups; fresh group, dry group, groups stored incell culture medium (2, 4, 7 days each), in milk (2, 4, 7 days each), and in saline (2, 4, 7 days each). Primaryculture of pulpal cells was conducted in each group and the success rates were compared by calculating thenumber of teeth with viable cells. The result of primary culture shows that the success rate decreases as the time of storage gets longer. Therewas no statistical difference between groups stored in the cell culture medium, milk, and saline for 2 and 4days. However, the groups stored in milk and saline for 7 days showed dramatic decrease in success rate comparedto the group stored in the cell culture medium. In conclusion, exfoliated or extracted deciduous teeth can be used to culture pulpal cells when they are storedin milk and saline for a certain period of time; however obtaining viable pulpal cells becomes harder as the storagetime gets longer.
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