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The Study of Lipid Proton Composition Change in a Rat Model of High Fat Diet Induced Fatty Liver by Magnetic Resonance Spectroscopy Analysis

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키워드

주제분류방사선과학

초록

The purpose of this study is to investigate the changes in lipid proton (LP) composition according to the induced obese fatty liver and to use it as basic data for treatment and diagnosis of fatty liver in the future. The phantom study was conducted to identify differences between STEAM and PRESS Pulse sequences in LP concentration. A high-fat diet (60%) was administered to eight Sprague-Dawley rats to induce obesity and fatty liver disease. Baseline magnetic resonance imaging /spectroscopy data were obtained prior to the introduction of high-fat diet, and data acquisition experiments were performed after eight weeks using procedures identical to those used for baseline studies. The six lipid proton metabolites were calculated using LCModel software. The correlation between the fat percentage and each LP, revealed that the methylene protons at 1.3 ppm showed the highest positive correlation. The α-methylene protons to carboxyl and diallylic protons showed negative correlation with fat percentage. The methylene proton showed the highest increase in the LP; however, it constituted only 71.86% of the total LP concentration. The methylene proton plays a leading role in fat accumulation in liver parenchyma.

Abstract

The purpose of this study is to investigate the changes in lipid proton (LP) composition according to the induced obese fatty liver and to use it as basic data for treatment and diagnosis of fatty liver in the future. The phantom study was conducted to identify differences between STEAM and PRESS Pulse sequences in LP concentration. A high-fat diet (60%) was administered to eight Sprague-Dawley rats to induce obesity and fatty liver disease. Baseline magnetic resonance imaging /spectroscopy data were obtained prior to the introduction of high-fat diet, and data acquisition experiments were performed after eight weeks using procedures identical to those used for baseline studies. The six lipid proton metabolites were calculated using LCModel software. The correlation between the fat percentage and each LP, revealed that the methylene protons at 1.3 ppm showed the highest positive correlation. The α-methylene protons to carboxyl and diallylic protons showed negative correlation with fat percentage. The methylene proton showed the highest increase in the LP; however, it constituted only 71.86% of the total LP concentration. The methylene proton plays a leading role in fat accumulation in liver parenchyma.

참고문헌 (36)

  1. Adams LA, "The natural history of nonalcoholic fatty liver disease : A population-based cohort study", Gastroenterology, 129(1), pp.113~121 (2005)
  2. Lieber CS, "Pathogenesis and treatment of alcoholic liver disease : Progress over the last 50 years", Rocz Akad Med Bialymst, 50, pp.7~20 (2005)
  3. Marengo A, "Progression and natural history of nonalcoholic fatty liver disease in adults", Clin Liver Dis, 20(2), pp.313~324 (2016)
  4. Sakamoto M., "1. Clinincal diagnosis and medical care of liver disease, present situation and progress", Nihon Hoshasen Gijutsu Gakkai Zasshi, 71(12), pp.1265~1274 (2015)
  5. Han S, "Effect of pure microsteatosis on transplant outcomes after living donor liver transplantation : A matched case-control study", Liver Transpl, 20(4), pp.473~482 (2014)
  6. Kang BK, "Hepatic fat quantification : A prospective comparison of magnetic resonance spectroscopy and analysis methods for chemical-shift gradient echo magnetic resonance imaging with histologic assessment as the reference standard", Invest Radiol, 47(6), pp.368~375 (2012)
  7. Kramer H, "Accuracy of Liver Fat Quantification With Advanced CT, MRI, and Ultrasound Techniques : Prospective Comparison with MR Spectroscopy", AJR Am J Roentgenol, 208(1), pp.92~100 (2017)
  8. Wells SA, "Quantification of hepatic fat and iron with magnetic resonance imaging", Magn Reson Imaging Clin N Am, 22(3), pp.397~416 (2014)
  9. Wierzbicki AS, "Nonalcoholic fatty liver disease and lipids", Curr Opin Lipidol, 23(4), pp.345~352 (2012)
  10. Hatta T, "Accurate and simple method for quantification of hepatic fat content using magnetic resonance imaging : A prospective study in biopsy-proven nonalcoholic fatty liver disease", J Gastroenterol, 45(12), pp.1263~1271 (2010)
  11. Malik R, "The clinical utility of biomarkers and the nonalcoholic steatohepatitis CRN liver biopsy scoring system in patients with nonalcoholic fatty liver disease", J Gastroenterol Hepatol, 24(4), pp.564~568 (2009)
  12. Adams LA, "Role of liver biopsy and serum markers of liver fibrosis in non-alcoholic fatty liver disease", Clin Liver Dis, 11(1), pp.25~35 (2007)
  13. Gavril RS, "Is liver biopsy necessary in patients with nonalcoholic fatty liver disease?", Rev Med Chir Soc Med Nat Iasi, 120(3), pp.503~507 (2016)
  14. Dang J, "Quantitative diagnosis of fatty liver using mask cover of CT image", Sichuan Da Xue Xue Bao Yi Xue Ban, 34(1), pp.158~159 (2003)
  15. Wang B, "Quantitative diagnosis of fatty liver with dual-energy CT : An experimental study in rabbits", Acta Radiol, 44(1), pp.92~97 (2003)
  16. Mendler MH, "Dual-energy CT in the diagnosis and quantification of fatty liver : Limited clinical value in comparison to ultrasound scan and single-energy CT, with special reference to iron overload", J Hepatol, 28(5), pp.785~794 (1998)
  17. Cutillo DP, "Absence of fatty replacement in radiation damaged liver : CT demonstration", J Comput Assist Tomogr, 13(2), pp.259~261 (1989)
  18. Ren J, "Composition of adipose tissue and marrow fat in humans by 1H NMR at 7 Tesla", J Lipid Res, 49(9), pp.2055~2062 (2008)
  19. Hamilton G, "Effect of PRESS and STEAM sequences on magnetic resonance spectroscopic liver fat quantification", J Magn Reson Imaging, 30(1), pp.145~152 (2009)
  20. Song KH, "Improved quantitative fatty acid values with correction of T2 relaxation time in terminal methyl group : In vivo proton magnetic resonance spectroscopy at ultra high field in hepatic steatosis", Chem Phys Lipids, 212, pp.35~43 (2018)
  21. Takahashi Y, "Animal models of nonalcoholic fatty liver disease", nonalcoholic steatohepatitis, 2012, pp.18(19), 2300~2308
  22. Fischbach F, "(1)H magnetic resonance spectroscopy (MRS) of the liver and hepatic malignant tumors at 3.0 Tesla", Radiologe, 44(12), pp.1192~1196 (2004)
  23. Fonvig CE, "1H-MRS Measured Ectopic Fat in Liver and Muscle in Danish Lean and Obese Children and Adolescents", PLoS One, 10(8), pp.e0135018~ (2015)
  24. Lee Y, "In vivo (1)H-MRS hepatic lipid profiling in nonalcoholic fatty liver disease: An animal study at 9.4 T", Magn Reson Med, 70(3), pp.620~629 (2013)
  25. Moreno A, "1H MRS markers of tumour growth in intrasplenic tumours and liver metastasis induced by injection of HT-29cells in nude mice spleen", NMR Biomed, 11(3), pp.93~106 (1998)
  26. Wang D, "1H magnetic resonance spectroscopy predicts hepatocellular carcinoma in a subset of patients with liver cirrhosis: A randomized trial", Medicine (Baltimore), 94(27), pp.e1066~ (2015)
  27. Yu SM, "Nonalcoholic fatty liver disease: Correlation of the liver parenchyma fatty acid with intravoxel incoherent motion MR imaging-an experimental study in a rat model", PLoS One, 10(10), pp.e0139874~ (2015)
  28. Dillman JR, "Quantitative liver MRI-biopsy correlation in pediatric and young adult patients with nonalcoholic fatty liver disease:Can one be used to predict the other?", AJR Am J Roentgenol, 210(1), pp.166~174 (2018)
  29. Dixon WT, "Simple proton spectroscopic imaging", Radiology, 153(1), pp.189~194 (1984)
  30. Lee SS, "Hepatic fat quantification using chemical shift MR imaging and MR spectroscopy in the presence of hepatic iron deposition : Validation in phantoms and in patients with chronic liver disease", J Magn Reson Imaging, 33(6), pp.1390~1398 (2011)
  31. Reeder SB, "On the performance of T2* correction methods for quantification of hepatic fat content", Magn Reson Med, 67(2), pp.389~404 (2012)
  32. Agten CA, "Quantification of early fatty infiltration of the rotator cuff muscles : Comparison of multi-echo Dixon with single-voxel MR spectroscopy", Eur Radiol, 26(10), pp.3719~3727 (2016)
  33. 유연화, "Comparison of Multi-Echo Dixon Methods with Volume Interpolated Breath-Hold Gradient Echo Magnetic Resonance Imaging in Fat-Signal Fraction Quantification of Paravertebral Muscle", Korean Journal of Radiology, 16(5), pp.1086~1095 (2015), 대한영상의학회[DOI]
  34. Ishizaka K, "Comparison of 1H MR spectroscopy, 3-point DIXON, and multi-echo gradient echo for measuring hepatic fat fraction", Magn Reson Med Sci, 10(1), pp.41~48 (2011)
  35. 민정환, "3.0T 자기공명영상을 이용한 해상력 특성에 대한 연구", 방사선기술과학, 43(4), pp.251~257 (2020), 대한방사선과학회
  36. 윤상준, "대뇌 종양에서 자기공명 분광법 적용 결과의 분석 연구", 방사선기술과학, 31(4), pp.355~366 (2008), 대한방사선과학회

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