β-Amyloid로 유도된 신경독성에 대한 熱多寒少湯 加感方의 抗치매효과
Protection effect of New-Yeolda-Hanso tang against β-Amyloid Induced Cytotoxicity in NGF-differentiated PC12 Cells
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1. Objectives Yeolda-Hanso tang (YH) has long been used as traditional herbal formula in Korea as various diseases. Now we modified Yeolda-Hanso tang (YH) for neurodegenerative diseases treatment and named New-Yeolda-Hanso tang (NYH). We investigated neuroprotective effects of NYH on NGF-differentiated PC12 cells cytotoxicity induced by β-Amyloid peptide (Aβ25-35) and evaluated the ability of NYH to prevent and treat for neurodegenerative diseases via autophagy enhancement. 2. Methods and Results 1) Protective effect of NYH on PC12 cells cytotoxity induced by Aβ25-35. PC12 cells survival was measured by MTT and lactate dehydrogenase (LDH) assay. 20μΜ β-Amyloid peptide (Aβ25-35) induced cytotoxicity on NGF-differentiated PC12 cells. NYH attenuated the cytotoxic effects of Aβ25-35 in a dose-dependent manner. 2) Pharmacological induction of Autophagy by NYH in PC12 cells Autophagy induction and activation was measured by immunoblot assay. Marker of autophagy, LC3Ⅱ expression and the ratio of LC3-II/I was slightly increased in the protein treated with YH, and significantly augmented in the protein treated with NYH. NYH-induced increase of LC3-II protein level was inhibited by 3MA. 3) Induction of Autophagy by NYH on Aβ25-35-induced injury in PC12 cells In MTT assay, 100㎍/㎖ pre-treated NYH attenuated 20μΜ Aβ25-35-induced cytotoxicity in PC12 cells. Protection effect of NYH was blocked by autophagy inhibitor 3MA. In immunoblot assay, 1200㎍/㎖ pre-treated NYH activated autophagy in 20μΜ Aβ25-35-induced cytotoxicity in PC12 cells. The observed effect was partially blocked by 3MA. 3. Conclusions All the results indicated that NYH possesses neuroprotective potential partially mediated by autophagy enhancement and NYH may be considered to be a promising new herbal formula to prevent and treat for neurodegenerative diseases including Alzheimer's disease (AD).
Abstract
1. Objectives Yeolda-Hanso tang (YH) has long been used as traditional herbal formula in Korea as various diseases. Now we modified Yeolda-Hanso tang (YH) for neurodegenerative diseases treatment and named New-Yeolda-Hanso tang (NYH). We investigated neuroprotective effects of NYH on NGF-differentiated PC12 cells cytotoxicity induced by β-Amyloid peptide (Aβ25-35) and evaluated the ability of NYH to prevent and treat for neurodegenerative diseases via autophagy enhancement. 2. Methods and Results 1) Protective effect of NYH on PC12 cells cytotoxity induced by Aβ25-35. PC12 cells survival was measured by MTT and lactate dehydrogenase (LDH) assay. 20μΜ β-Amyloid peptide (Aβ25-35) induced cytotoxicity on NGF-differentiated PC12 cells. NYH attenuated the cytotoxic effects of Aβ25-35 in a dose-dependent manner. 2) Pharmacological induction of Autophagy by NYH in PC12 cells Autophagy induction and activation was measured by immunoblot assay. Marker of autophagy, LC3Ⅱ expression and the ratio of LC3-II/I was slightly increased in the protein treated with YH, and significantly augmented in the protein treated with NYH. NYH-induced increase of LC3-II protein level was inhibited by 3MA. 3) Induction of Autophagy by NYH on Aβ25-35-induced injury in PC12 cells In MTT assay, 100㎍/㎖ pre-treated NYH attenuated 20μΜ Aβ25-35-induced cytotoxicity in PC12 cells. Protection effect of NYH was blocked by autophagy inhibitor 3MA. In immunoblot assay, 1200㎍/㎖ pre-treated NYH activated autophagy in 20μΜ Aβ25-35-induced cytotoxicity in PC12 cells. The observed effect was partially blocked by 3MA. 3. Conclusions All the results indicated that NYH possesses neuroprotective potential partially mediated by autophagy enhancement and NYH may be considered to be a promising new herbal formula to prevent and treat for neurodegenerative diseases including Alzheimer's disease (AD).
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