太陰人 熱多寒少湯이 3T3-L1 脂肪前驅細胞 分化에 미치는 影響
Effects of Yeoldahanso-tang, a Sasang Constitutional Herbal Prescription for Taeeumin, on the Adipogenesis in 3T3-L1 Cells
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Objectives Although Yeoldahanso-tang (YDHST) has been widely used for treatment of obesity and its related diseases such as hyperinsulinemia and hypertension for Taeeumin, no scientific evidence has reported yet to support its ability to work against these metabolic disorders. Our study was aimed to investigate the anti-obesity effect of YDHST extract on the cellular differentiation of 3T3-L1 preadipocytes into adipocytes. Methods 3T3-L1 preadipocytes were differentiated into adipocytes by adding insulin, dexamethasone and 3-isobutyl-1-methylxanthine (IBMX) for 8 days in the absence or presence of YDHST extract. Anti-obesity effects of YDHST extract were evaluated by Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, triglyceride contents, and leptin production. Results YDHST extract remarkably prevented lipid accumulation with no cytotoxicity in the differentiated 3T3-L1 cells. In addition, YDHST extract decreased contents of triglyceride 3T3-L1 adipocytes. Consistently, YDHST extract caused a significant inhibition of GPDH activity and leptin production in a dose-dependent manner. Conclusions Our findings suggest that Sasang constitutional herbal formula YDHST for Tae-eumin has anti-obesity activity by regulation of the adipogenesis process in vitro. Additional study will be required to further confirm the inhibitory effect on adipocyte differentiation by using in vivo animal model.
Abstract
Objectives Although Yeoldahanso-tang (YDHST) has been widely used for treatment of obesity and its related diseases such as hyperinsulinemia and hypertension for Taeeumin, no scientific evidence has reported yet to support its ability to work against these metabolic disorders. Our study was aimed to investigate the anti-obesity effect of YDHST extract on the cellular differentiation of 3T3-L1 preadipocytes into adipocytes. Methods 3T3-L1 preadipocytes were differentiated into adipocytes by adding insulin, dexamethasone and 3-isobutyl-1-methylxanthine (IBMX) for 8 days in the absence or presence of YDHST extract. Anti-obesity effects of YDHST extract were evaluated by Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, triglyceride contents, and leptin production. Results YDHST extract remarkably prevented lipid accumulation with no cytotoxicity in the differentiated 3T3-L1 cells. In addition, YDHST extract decreased contents of triglyceride 3T3-L1 adipocytes. Consistently, YDHST extract caused a significant inhibition of GPDH activity and leptin production in a dose-dependent manner. Conclusions Our findings suggest that Sasang constitutional herbal formula YDHST for Tae-eumin has anti-obesity activity by regulation of the adipogenesis process in vitro. Additional study will be required to further confirm the inhibitory effect on adipocyte differentiation by using in vivo animal model.
참고문헌 (31)
- MA WD, "Plain Questions of Huangdi's Internal Classic", pp.224~ (1975), Seongbosa
- National federation of department of sasang constitutional medicine, colleges of oriental medicine, "Sasang constitutional medicine. 2nd Ed", pp.638~639 (2004), Jipmoondang
- Kim DR, "Clinical study on corelation of obesity and Sasang constitution", J Sasang Constitut Med, 8(1), pp.219~ (1996)
- Cho MS, "A Clinical Study of the objesity patients according to the Sasang Constitution", J Sasang Constitut Med, 10(2), pp.485~511 (1998)
- Shin DJ, "The effect of Taeumjowetang and herba ephedrae on the obese mRNA and TMF-α mRNA in obesity-induced rats", J Sasang Constitut Med, 13(1), pp.79~87 (2001)
- Won JS, "New Compilation of Four constitutional Medicine" (1974), Jonghab-uiwonsa
- Green, H, "An established pre-adipose cell line and its differentiation in culture", Cell, 3, pp.127~133 (1974)
- Sahai A, "Chronic hypoxia impairs the differentiation of 3T3-L1 fibroblast in culture: role of sustained protein kinase C activation", Journal of Cell Physiology, 160, pp.107~112 (1994)
- "National Health and Nutrition Examination Survey Report"[URL]
- Buyukhatipoglu H, "A possibly overlooked side effect of Orlistat: gastroesophageal reflux disease", J Natl Med Assoc, 100(10), pp.1207~ (2008)
- Van der Zwaal EM, "Olanzapine and sibutramine have opposing effects on the motivation for palatable food", Behav Pharmacol, 23(2), pp.198~204 (2012)
- P Cornelius, "Regulation of Adipocyte Development Annual Review of Nutrition", 14, pp.99~129 (1994)
- Shao D, "Interdomain communication regulating ligand binding by PPAR-gamma", Nature, 396, pp.377~380 (1998)
- He ML, "Effect of a seaweed extract on fatty acid accumulation and glycerol-3-phosphate dehydrogenase activity in 3T3-L1 adipocytes", Lipids, 44(2), pp.125~132 (2009)
- Zhang F, "Crystal structure of the obese protein leptin-E100", Nature, 387(6629), pp.206~209 (1997)
- Maffei M, "Leptin levels in human and rodent : measurement of plasma leptin and ob RNA in obese and weight-reduced subjects", Nat Med, 1(11), pp.1155~1161 (1995)
- Zhang W, "Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet", Eur J Pharmacol, 649(1-3), pp.398~402 (2010)
- Song KH, "Extracts of Scutellaria baicalensis Reduced Body Weight and Blood Triglyceride in db", db Mice, ~
- Jong Rok Lee, "Anti-obese and Hypolipemic Effects of the Aqueous Extracts of Raphani Semen in Mice Fed High Fat Diet", Journal of the Korean Society for Applied Biological Chemistry, 52(1), pp.50~57 (2009), 한국응용생명화학회
- Lee CE, "Long-Term Consumption of Platycodi Radix Ameliorates Obesity and Insulin Resistance via the Activation of AMPK Pathways", Evid Based Complement Alternat Med (2012)
- Kwon DY, "Platyconic acid, a saponin from Platycodi radix, improves glucose homeostasis by enhancing insulin sensitivity in vitro and in vivo", Eur J Nutr, 51(5), pp.529~540 (2012)
- 한소라, "Herbal extract THI improves metabolic abnormality in mice fed a high-fat diet", Nutrition Research and Practice, 5(3), pp.198~204 (2011), 한국영양학회
- Bitou, N, "Screening of lipase inhibitors from marine algae", Lipids, 34, pp.441~445 (1999)
- National Institute of Health Consensus Development Conference Statement, "Health implacation of obesity", Ann intern Med, 130, pp.147~151 (1985)
- 김중화, "사상체질별 약물의 lipase 저해활성을 통한 항비만효능에 관한 실험적 연구", 동의생리병리학회지, 19(3), pp.710~715 (2005), 대한동의병리학회
- Ikarashi N, "Inhibition of preadipocyte differentiation and lipid accumulation by Orengedokuto treatment of 3T3-L1 cultures", Phytother Res, 26(1), pp.91~100 (2012)
- 차민경, "3T3-L1 cell에서 방풍통성산의 지방세포 분화 억제 효과 연구", 대한한방소아과학회지, 20(2), pp.177~196 (2006), 대한한방소아과학회
- Akagiri S, "Bofutsushosan, an Oriental Herbal Medicine, Attenuates the Weight Gain of White Adipose Tissue and the Increased Size of Adipocytes Associated with the Increase in Their Expression of Uncoupling Protein 1 in High-Fat Diet-Fed Male KK", Ta mice, 2008, pp.42(2), 158~166
- 김은영, "태음인 청폐사간탕이 lipase 활성저해 및 db", db mouse의 체중감량, 혈장지질, UCP 1, 2 발현에 미치는 영향, 2007, pp.19(1), 171~185[DOI]
- Li M, "Hepatic PTP1B expression involvement in the effects of Chinese medicine formula xiao-gao-jiang-zhuo using an obese rat model", Am J Chin Med, 39(2), pp.301~313 (2011)