이차구개판 발생 동안 나타나는 Tbx3와 Bmp4의 발현양상의 의미와 조직 상호작용에 대한 연구

Abstract

Craniofacial malfonnation is the most common birth defects that occur in hurn머1S. During the secondary palate development, two shelves elevated to a horizontal position above the tongue, then they completely fused in developing embryo. In this study, the precise morphological changes in control and reverse culture (180 degree rotation) of the developing palate were examined that could find the key features of morphological differences between control and reverse culture. Moreover, for reveal the function of epithelium in palatogenesis, we removed and recombined palatal epithelium. our results show that epithelial-mesenchymal interactions are essential to π，x3 and Bm어 signalling because after remove the palatal epithelium, expression of Tbx3 and Bmp4 were not detected in developing 며late. The present study shows interaction between these molecules in palatal development. Furthennore, our results demonstrate that development of the anterior palate may have completely different signalling network independently of development in the posterior palate.