Localization of Eruption-Related Molecules in Developing Rat Incisors
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
A complex and intricate cascades of gene expression is necessary for tooth development, and tooth eruption is one of main events during tooth development. Tooth eruption is considered to be strictly controlled either by signaling or extracellular molecules that are secreted by the tooth germs themselves. There have been numerous reports on the eruption-related molecules; however, there is still a lack of information regarding the preparation of the eruption pathway and the key tissue factors involved. This study aimed to localize the eruption-related molecules such as cyclophilin A (Cyp-A), extracellular matrix metalloproteinase inducer (EMMPRIN), matrix metalloproteinase proteins (MMP-9), receptor activator of nuclear factor kappa-B ligand (RANKL), and osteoprotegerin (OPG) in the developing rat incisors. Immunofluorescence findings revealed that Cyp-A, EMMPRIN, OPG, and RANKL were expressed strongly in the dental lamina epithelium of the rat incisor germs during eruptive movement. OPG was also expressed in the osteoclasts in the apical region. MMP-9, a final tissue resolution molecule, was more widely localized in the area surrounding the erupting incisor. This study suggested that the dental lamina epithelium may play a key role in preparing eruption pathways for the incisor in rats.
Abstract
A complex and intricate cascades of gene expression is necessary for tooth development, and tooth eruption is one of main events during tooth development. Tooth eruption is considered to be strictly controlled either by signaling or extracellular molecules that are secreted by the tooth germs themselves. There have been numerous reports on the eruption-related molecules; however, there is still a lack of information regarding the preparation of the eruption pathway and the key tissue factors involved. This study aimed to localize the eruption-related molecules such as cyclophilin A (Cyp-A), extracellular matrix metalloproteinase inducer (EMMPRIN), matrix metalloproteinase proteins (MMP-9), receptor activator of nuclear factor kappa-B ligand (RANKL), and osteoprotegerin (OPG) in the developing rat incisors. Immunofluorescence findings revealed that Cyp-A, EMMPRIN, OPG, and RANKL were expressed strongly in the dental lamina epithelium of the rat incisor germs during eruptive movement. OPG was also expressed in the osteoclasts in the apical region. MMP-9, a final tissue resolution molecule, was more widely localized in the area surrounding the erupting incisor. This study suggested that the dental lamina epithelium may play a key role in preparing eruption pathways for the incisor in rats.
참고문헌 (17)
- Tetsuro Sameshima, "Glioma cell extracellular matrix metalloproteinase inducer (EMMPRIN) (CD147) stimulates production of membrane-type matrix metalloproteinases and activated gelatinase A in co-cultures with brain-derived fibroblasts", Cancer Letters, 157(2), pp.177~184 (2000), Elsevier BV[DOI]
- Johanna Pispa, "Mechanisms of ectodermal organogenesis", Developmental Biology, 262(2), pp.195~205 (2003), Elsevier BV[DOI]
- Vyacheslav Yurchenko, "CD147 Is a Signaling Receptor for Cyclophilin B", Biochemical and Biophysical Research Communications, 288(4), pp.786~788 (2001), Elsevier BV[DOI]
- P Nigro, "Cyclophilin A: a key player for human disease", Cell Death & Disease, 4(10), pp.e888~e888 (2013), Springer Science and Business Media LLC[DOI]
- Takahiro Oshiro, "Osteoclast induction in periodontal tissue during experimental movement of incisors in osteoprotegerin-deficient mice", The Anatomical Record, 266(4), pp.218~225 (2002), Wiley[DOI]
- Sandrine Theoleyre, "The molecular triad OPG", RANK, Cytokine & Growth Factor Reviews, pp.2004, 15(6)[DOI]
- T. John Martin, "RANKL", OPG; Critical role in bone physiology, 2015, pp.16(2), 131~139[DOI]
- Dawen Liu, "MyD88 expression in the rat dental follicle: implications for osteoclastogenesis and tooth eruption", European Journal of Oral Sciences, 118(4), pp.333~341 (2010), Wiley[DOI]
- Paulo Sérgio Cerri, "Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluation", Journal of Anatomy, 217, pp.116~125 (2010), Wiley[DOI]
- 김성곤, "Downregulation of matrix metalloproteinases in hyperplasticdental follicles results in abnormal tooth eruption", BMB reports, 41(4), pp.322~327 (2008), 한국생화학분자생물학회
- KASINRERK, "CD147 monoclonal antibodies induce homotypic cell aggregation of monocytic cell line U937 via LFA‐1", ICAM‐1 pathway, 2001, pp.96(2), 184~192[DOI]
- Yang Y, "Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes", macrophages via CD147 signaling pathway in rheumatoid arthritis, 2008, pp.47, 1299~1310
- Ludmila M. Khromykh, "Cyclophilin A produced by thymocytes regulates the migration of murine bone marrow cells", Cellular Immunology, 249(1), pp.46~53 (2007), Elsevier BV[DOI]
- Andersen H, "A proteome study of secreted prostatic factors affecting osteoblastic activity: identification and characterization of cyclophilin A", Eu J Cancer, 39, pp.989~995 (2003)
- Se-Hwa Kim, "Cyclophilin A as a Novel Biphasic Mediator of Endothelial Activation and Dysfunction", The American Journal of Pathology, 164(5), pp.1567~1574 (2004), Elsevier BV[DOI]
- J.H. Kang, "Osteoprotegerin Expressed by Osteoclasts: An Autoregulator of Osteoclastogenesis", Journal of Dental Research, 93(11), pp.1116~1123 (2014), SAGE Publications[DOI]
- Gary E. Wise, "Requirement of alveolar bone formation for eruption of rat molars", European Journal of Oral Sciences, 119(5), pp.333~338 (2011), Wiley[DOI]