Regulation of Osteoprotegerin and Receptor activator of nuclear factor kappa-Β ligand in Rat Molar Eruption
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The eruptive movement of tooth germs in the alveolar bone is supposed to be timely regulated by molecules that involve the differentiation of osteoclasts for preparing a tooth eruption pathway. Thus, the detection of the molecules is the first to understand the movement. This study hypothesized that molecules are expressed differentially from tooth germs themselves with follicles in eruptive movement. To verify this, genes were detected in rat molar germs using differential display-PCR. The localization and expression of the detected molecules were evidenced by immunofluorescence, and real-time PCR, and western blot, respectively. Osteoprotegerin was one of the differentially expressed molecules between the cap stage (third molar germs before the eruptive movement) and the root formation stage (2nd molar germs after the eruptive movement) at postnatal day nine. Osteoprotegerin was localized in follicular and perifollicular tissues, which are overlaying developing the third molar germs. The osteoprotegerin expression was downregulated from day three to nine in a time dependent manner, followed by the upregulation at day 12. In contrast, receptor activator of nuclear factor kappa-Β ligand was expressed strongly in follicular tissues overlaying the second molar germs, and up-regulated in time-dependently. The treatment of alendronate, a second-generation bisphosphonate for nine days, revealed the upregulation of osteoprotegerin and downregulation of receptor activator of nuclear factor kappa-Β ligand. These results suggest that dental follicles may control the eruptive movement of tooth germs by regulating receptor activator of nuclear factor kappa-Β ligand and osteoprotegerin expression in dental follicles.
Abstract
The eruptive movement of tooth germs in the alveolar bone is supposed to be timely regulated by molecules that involve the differentiation of osteoclasts for preparing a tooth eruption pathway. Thus, the detection of the molecules is the first to understand the movement. This study hypothesized that molecules are expressed differentially from tooth germs themselves with follicles in eruptive movement. To verify this, genes were detected in rat molar germs using differential display-PCR. The localization and expression of the detected molecules were evidenced by immunofluorescence, and real-time PCR, and western blot, respectively. Osteoprotegerin was one of the differentially expressed molecules between the cap stage (third molar germs before the eruptive movement) and the root formation stage (2nd molar germs after the eruptive movement) at postnatal day nine. Osteoprotegerin was localized in follicular and perifollicular tissues, which are overlaying developing the third molar germs. The osteoprotegerin expression was downregulated from day three to nine in a time dependent manner, followed by the upregulation at day 12. In contrast, receptor activator of nuclear factor kappa-Β ligand was expressed strongly in follicular tissues overlaying the second molar germs, and up-regulated in time-dependently. The treatment of alendronate, a second-generation bisphosphonate for nine days, revealed the upregulation of osteoprotegerin and downregulation of receptor activator of nuclear factor kappa-Β ligand. These results suggest that dental follicles may control the eruptive movement of tooth germs by regulating receptor activator of nuclear factor kappa-Β ligand and osteoprotegerin expression in dental follicles.
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