Differential Localization of ODAM in Periodontitis with Similar Clinical Severities
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초록
Odontogenic ameloblast-associated protein (ODAM) reportedly plays an important role in maintaining periodontal integrity. We aimed to investigate the cause of different ODAM values in the gingival crevicular fluid (GCF) and discrepancies between GCF ODAM values and histological ODAM distribution in periodontal disease. ODAM concentration in the GCF of nine adult patients with severe periodontitis and ODAM distribution in the gingival tissues of three adult patients were analyzed using enzyme-linked immunosorbent assay and immunohistochemistry (IHC), respectively. ODAM in the GCF was detected in six samples but not in the remaining three samples; no differences were observed in the clinical characteristics between the ODAM-positive and ODAM-negative groups. ODAM was observed in the junctional epithelium of healthy gingival tissue by IHC analyses. There were two different ODAM expression patterns in gingival tissues with periodontitis of similar clinical severities, no overall expression or localized overexpression in the connective tissue layer. ODAM in severe periodontitis was secreted into the GCF or otherwise released into the connective tissue. Although clinically diagnosed as the same degree of periodontitis, the expression pattern of ODAM may differ according to whether ODAM can function as a junctional protein.
Abstract
Odontogenic ameloblast-associated protein (ODAM) reportedly plays an important role in maintaining periodontal integrity. We aimed to investigate the cause of different ODAM values in the gingival crevicular fluid (GCF) and discrepancies between GCF ODAM values and histological ODAM distribution in periodontal disease. ODAM concentration in the GCF of nine adult patients with severe periodontitis and ODAM distribution in the gingival tissues of three adult patients were analyzed using enzyme-linked immunosorbent assay and immunohistochemistry (IHC), respectively. ODAM in the GCF was detected in six samples but not in the remaining three samples; no differences were observed in the clinical characteristics between the ODAM-positive and ODAM-negative groups. ODAM was observed in the junctional epithelium of healthy gingival tissue by IHC analyses. There were two different ODAM expression patterns in gingival tissues with periodontitis of similar clinical severities, no overall expression or localized overexpression in the connective tissue layer. ODAM in severe periodontitis was secreted into the GCF or otherwise released into the connective tissue. Although clinically diagnosed as the same degree of periodontitis, the expression pattern of ODAM may differ according to whether ODAM can function as a junctional protein.
참고문헌 (24)
- Nishio C, "Expression pattern of odontogenic ameloblast-associated and amelotin during formation and regeneration of the junctional epithelium", European Cells and Materials, 20, pp.393~402 (2010)[DOI]
- Wazen RM, "Inactivation of the Odontogenic ameloblast-associated gene affects the integrity of the junctional epithelium and gingival healing", European Cells and Materials, 30, pp.187~199 (2015)[DOI]
- Lee HK, "Odontogenic Ameloblast-associated Protein (ODAM) Mediates Junctional Epithelium Attachment to Teeth via Integrin-ODAM-Rho Guanine Nucleotide Exchange Factor 5 (ARHGEF5)-RhoA Signaling", J Biol Chem, 290(23), pp.14740~14753 (2015)[DOI]
- Kawasaki K, "The SCPP gene family and the complexity of hard tissues in vertebrates", Cells Tissues Organs, 194(2-4), pp.108~112 (2011)[DOI]
- Park JC, "The amyloid protein APin is highly expressed during enamel mineralization and maturation in rat incisors", Eur J Oral Sci, 115(2), pp.153~160 (2007)[DOI]
- Moffatt P, "Characterization of Apin, a secreted protein highly expressed in tooth-associated epithelia", J Cell Biochem, 103(3), pp.941~956 (2008)[DOI]
- Lee HK, "Expression pattern, subcellular localization, and functional implications of ODAM in ameloblasts, odontoblasts, osteoblasts, and various cancer cells", Gene Expr Patterns, 12(3-4), pp.102~108 (2012)[DOI]
- Gandhi SS, "Nuclear Odontogenic Ameloblast-Associated Protein(ODAM)Correlates with Melanoma Sentinel Lymph Node Metastasis", Journal of Cancer Therapy, 04(08), pp.1283~1289 (2013)[DOI]
- Lee HK, "Odontogenic ameloblasts-associated protein(ODAM), via phosphorylation by bone morphogenetic protein receptor type IB(BMPR-IB), is implicated in ameloblast differentiation", J Cell Biochem, 113(5), pp.1754~1765 (2012)[DOI]
- Lee HK, "The odontogenic ameloblast-associated protein(ODAM)cooperates with RUNX2 and modulates enamel mineralization via regulation of MMP-20", J Cell Biochem, 111(3), pp.755~767 (2010)[DOI]
- Lee HK, "Odontogenic ameloblast-associated protein(ODAM)in gingival crevicular fluid for site-specific diagnostic value of periodontitis : a pilot study", BMC Oral Health, 18(1), pp.148~ (2018)[DOI]
- Nakayama Y, "Localization and expression pattern of amelotin, odontogenic ameloblast-associated protein and follicular dendritic cell-secreted protein in the junctional epithelium of inflamed gingiva", Odontology, 105(3), pp.329~337 (2017)[DOI]
- Nazir MA, "Prevalence of periodontal disease, its association with systemic diseases and prevention", Int J Health Sci(Qassim), 11(2), pp.72~80 (2017)
- Kestler DP, "ODAM Expression Inhibits Human Breast Cancer Tumorigenesis", Breast Cancer(Auckl), 5, pp.73~85 (2011)[DOI]
- Yu M, "Odontogenic ameloblast-associated protein(ODAM)inhibits human colorectal cancer growth by promoting PTEN elevation and inactivating PI3K", AKT signaling, 2016, pp.84, 601~607
- Kestler DP, "Expression of odontogenic ameloblast-associated protein (ODAM) in dental and other epithelial neoplasms", Mol Med, 14(5-6), pp.318~326 (2008)[DOI]
- Listgarten MA, "Pathogenesis of periodontitis", J Clin Periodontol, 13(5), pp.418~430 (1986)[DOI]
- Bosshardt DD, "The periodontal pocket: pathogenesis, histopathology and consequences", Periodontol 2000, 76(1), pp.43~50 (2018)[DOI]
- L. Pihlstrom B, "Periodontal risk assessment, diagnosis and treatment planning", Periodontol 2000, 25, pp.37~58 (2001)[DOI]
- Greenstein G, "Contemporary interpretation of probing depth assessments: diagnostic and therapeutic implications", A literature review. J Periodontol., 68(12), pp.1194~1205 (1997)[DOI]
- Li X, "Systemic diseases caused by oral infection", Clin Microbiol Rev, 13(4), pp.547~558 (2000)[DOI]
- Meyer MS, "A review of the relationship between tooth loss, periodontal disease, and cancer", Cancer Causes Control, 19(9), pp.895~907 (2008)[DOI]
- Frank A. Scannapieco, "Associations Between Periodontal Disease and Risk for Atherosclerosis, Cardiovascular Disease, and Stroke. A Systematic Review", Annals of Periodontology, 8(1), pp.38~53 (2003), Wiley[DOI]
- Grossi SG, "Periodontal Disease and Diabetes Mellitus: A Two-Way Relationship", Annals of Periodontology, 3(1), pp.51~61 (1998)[DOI]