SCC 25 세포에 대한 승마추출물의 caspase 3 활성을 통한 세포자멸사 유도
Cimicifuga rhizome extract induces apoptosis through caspase-3 activation in SCC 25 cells
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본 연구는 승마추출물(cimicifuga rhizome )을 이용하여 구강편평상피암(oral squamous cell carcinoma, SCC 25 ) 세포에서의 세포 사멸 유도 및 경로를 확인하기 위해 수행되었다. 본 연구에서는 SCC 25 세포에서 승마추출물에 대한 세포 생존력을 검출하기 위해 WST-1 분석을 사용하였다. 면역 형광 염색법 및 유세포 분석법은 구강암 세포의 세포 자멸사를 검출하기 위해 사용되었다. 그 후, 승마추출물이 세포 사멸과 관련하여 단백질(PARP-1, caspase 3 및 caspase 9)에 미치는 영향을 단백질 면역 분석법으로 확인하였다. 본 저자들은 승마 추출물이 SCC 25 세포에서 농도 의존적 방식으로 세포 생존력을 유의하게 억제한다는 것을 발견하였다. 또한 승마추출물은 sub G1을 증가시키고 cytochrome c와 AIF 위치를 변경하였다. 또한, caspase-3 신호 전달 경로가 승마추출물의 의해 유도된 세포 사멸에서 핵심적인 역할을 한다는 것을 관찰하였다. 본 연구 결과 승마추출물이 SCC 25 세포에서 caspase-3 신호 전달 경로를 조절하여 세포 사멸을 촉진하는 것으로 나타났다.
Abstract
This study was undertaken to verify the induction and pathway of apoptosis in oral squamous carcinoma cells (SCC 25) using cimicifuga rhizome extract. In the present study, the WST-1 assay was used to detect the cell viability of cimicifuga rhizome extract on SCC 25 cells. Immuno-fluorescence and flow cytometry assays were used to detect cell apoptosis. The effect of cimicifuga rhizome extract on apoptosis-associated proteins (PARP-1, caspase-3 and caspase-9) were determined by western blot. We found that cimicifuga rhizome extract significantly inhibited cell viability in a dose-dependent manner in SCC 25 cells. Also, cimicifuga rhizome extract increased the sub G1 and changed the cytochrome c and AIF location. In addition, we observed that the caspase-3 signaling pathway played a key role in cimicifuga rhizome extract induced apoptosis. The results of the present study showed that cimicifuga rhizome extract promotes apoptosis via regulating the caspase-3 signaling pathway in SCC 25 cells.
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