에탄올에 의해 추출한 황금이 구강암 세포에서 나타나는 자가포식작용
Effect of autophagy in human tongue squamous cell carcinoma SCC 25 cells from Scutellariae Radix by ethanol extract
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초록
Objectives : The purpose of the study is to examine the cell growth effect and autophagyeffect of Scutellariae Radix by ethanol extract in SCC 25 cells. Methods : Cell growth inhibitory effect and autophagy induced by Scutellariae Radix wereconfirmed by WST-1 assay, monodansylcadaverine(MDC) stain, and flow cytometry by acridineorange(AO) stain. Results : The Scutellariae Radix treatment decreased the cell proliferation in a dose and timedependent manner. Scutellariae Radix has anticancer effects that autophagic vacuoles were apparentby MDC and AO staining in SCC 25 cells. Conclusions : Scutellariae Radix showed anticancer activity against SCC 25 cells via autophagy. The data provided the possibility that Scutellariae Radix may potentially contribute to oral cancertreatment.
Abstract
Objectives : The purpose of the study is to examine the cell growth effect and autophagyeffect of Scutellariae Radix by ethanol extract in SCC 25 cells. Methods : Cell growth inhibitory effect and autophagy induced by Scutellariae Radix wereconfirmed by WST-1 assay, monodansylcadaverine(MDC) stain, and flow cytometry by acridineorange(AO) stain. Results : The Scutellariae Radix treatment decreased the cell proliferation in a dose and timedependent manner. Scutellariae Radix has anticancer effects that autophagic vacuoles were apparentby MDC and AO staining in SCC 25 cells. Conclusions : Scutellariae Radix showed anticancer activity against SCC 25 cells via autophagy. The data provided the possibility that Scutellariae Radix may potentially contribute to oral cancertreatment.
참고문헌 (28)
- 김창숙, "우리나라 국민기초생활수급자의 사회경제적인 특성과구강건강에 관한 연구", 한국치위생학회지, 13(6), pp.995~1004 (2013), 한국치위생학회[DOI]
- 천혜원, "우리나라 성인의 구강질환과 만성질환의 관련성", 한국치위생학회지, 12(2), pp.235~249 (2012), 한국치위생학회[DOI]
- Agostini M, "The fatty acid synthase inhibitor orlistat reduces the growth and metastasis of orthotopic tongue oral squamous cell carcinomas", Mol Cancer Ther (2013)
- Besic Gyenge E, "Uptake and fate of surface modified silica nanoparticles in head and neck squamous cell carcinoma", J Nanobiotechnology, 9, pp.32~ (2011)[DOI]
- Hong GE, "Flavonoids Identified from Korean Scutellaria baicalensis Georgi Inhibit Inflammatory Signaling by Suppressing Activation of NF- κ B and MAPK in RAW 264.7 Cells", Evid Based Complement Alternat Med, 2013, pp.912031~ (2013)[DOI]
- Wang CZ, "Hydrophobic flavonoids from Scutellaria baicalensis induce colorectal cancer cell apoptosis through a mitochondrialmediated pathway", Int J Oncol, 42(3), pp.1018~1026 (2013)[DOI]
- Li C, "Pharmacological effects and pharmacokinetics properties of Radix Scutellariae and its bioactive flavones", Biopharm Drug Dispos, 32(8), pp.427~445 (2011)[DOI]
- Jung HS, "Antiallergic effects of Scutellaria baicalensis on inflammation in vivo and in vitro", J Ethnopharmacol, 141(1), pp.345~349 (2012)[DOI]
- Kim EH, "Anti-inflammatory effects of Scutellaria baicalensis extract via suppression of immune modulators and MAP kinase signaling molecules", JOURNAL OF ETHNOPHARMACOLOGY, 126, pp.320~331 (2009), ELSEVIER IRELAND LTD[DOI]
- Choi J, "Flavones from scutellaria baicalensis georgi attenurgi apoptcais and Gragiin oxidation in neuranal cell lines", Biochim Biophys Acta, 1571(3), pp.201~210 (2002)
- Song HR, "Scutellaria flavonoid supplementation reverses ageing-related cognitive impairment and neuronal changes in aged rats", Brain Inj, 23(2), pp.146~153 (2009)[DOI]
- Paglin S, "A novel response of cancer cells to radiation involves autophagy and formation of acidic vesicles", Cancer Res, 61(2), pp.439~444 (2001)
- Carson DA, "Apoptosis and disease", Lancet, 341(8855), pp.1251~1254 (1993)
- Zhang Y, "Oxymatrine induces mitochondria dependent apoptosis in human osteosarcoma MNNG", HOS cells through inhibition of PI3K, Tumour Biol, pp.2013[DOI]
- Elumalai P, "Induction of apoptosis in human breast cancer cells by nimbolide through extrinsic and intrinsic pathway", Toxicol Lett, 215(2), pp.131~142 (2012)[DOI]
- S.J. Riedl, "The apoptosome: signalling platform of cell death", Nat Rev Mol Cell Biol, 8(5), pp.405~413 (2007)
- Kim AD, "A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells", Cell Death Dis, 1(4), pp.e750~ (2013)[DOI]
- Amaravadi RK, "Principles and current strategies for targeting autophagy for cancer treatment", Clin Cancer Res, 17(4), pp.654~666 (2011)[DOI]
- Long JS, "New frontiers in promoting tumour cell death: targeting apoptosis, necroptosis and autophagy", Oncogene, 31(49), pp.5045~5060 (2012)[DOI]
- Levine B, "Autophagy in cell death: an innocent convict?", J Clin Invest, 115(10), pp.2679~2688 (2005)
- Kumar D, "Rottlerin-induced autophagy leads to the apoptosis in breast cancer stem cells: molecular mechanisms", Mol Cancer, 12(1), pp.171~ (2013)[DOI]
- Zhang DY, "Inhibition of cancer cell proliferation and prostaglandin E2 synthesis by Scutellaria baicalensis", Cancer Res, 63(14), pp.4037~4043 (2003)
- Gao J, "The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells", Toxicol Appl Pharmacol, 254(3), pp.221~228 (2011)[DOI]
- Pan TL, "Inhibitory effects of Scutellaria baicalensis extract on hepatic stellate cells through inducing G2", M cell cycle arrest and activating ERK-dependent apoptosis via Bax and caspase pathway, 2012, pp.139(3), 829~837
- Kumagai T, "Scutellaria baicalensis, a herbal medicine: anti-proliferative and apoptotic activity against acute lymphocytic leukemia, lymphoma and myeloma cell lines", Leuk Res, 31(4), pp.523~530 (2007)
- Lin C, "AKT serine", threonine protein kinase modulates baicalin-triggered autophagy in human bladder cancer T24 cells, 2013, pp.42(3), 993~1000
- Bai LY, "G15, a GPR30 antagonist, induces apoptosis and autophagy in human oral squamous carcinoma cells", Chem Biol Interact, 206(2), pp.375~384 (2013)[DOI]
- Kim JY, "Curcumin-induced autophagy contributes to the decreased survival of oral cancer cells", Arch Oral Biol, 57(8), pp.1018~1025 (2012)[DOI]
