Role of Mechanical Force-Induced Secretory Leukocyte Peptidase Inhibitor in Periodontal Ligament Cells
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Multiple genes are involved in periodontal tissue remodeling in orthodontic tooth movement (OTM). Studies have attempted to establish the precise mechanism of remodeling during OTM. This study investigated the mechanism of action and functional roles of secretory leukocyte peptidase inhibitor (SLPI), a molecule enhancing tissue repair during OTM. The upper first molars of rats were moved mesially by applying a Ni-Ti coil spring on the upper incisors as an anchor. Primary cultures were prepared from human periodontal ligament (HPDL) cells and subjected to tension and compression forces. In vivo: The SLPI level increased at 1d, 2d, and 6d after OTM by real time-PCR and Western blot. Immunoreactivity against SLPI was mainly found in the PDL and enhanced in the tension and compression side. In vitro: SLPI worked by acting on MAPK pathways including ERK and p38. SLPI mRNA was upregulated by tension or compression. SLPI siRNA treatment induced upregulation of IL-6 and IL-8 in HPDL cells during the tension applications. However, SLPI siRNA treatment induced the downregulation of IL-8 during compression application. These results suggest that SLPI in the PDL may be involved in inflammation control in OTM.
Abstract
Multiple genes are involved in periodontal tissue remodeling in orthodontic tooth movement (OTM). Studies have attempted to establish the precise mechanism of remodeling during OTM. This study investigated the mechanism of action and functional roles of secretory leukocyte peptidase inhibitor (SLPI), a molecule enhancing tissue repair during OTM. The upper first molars of rats were moved mesially by applying a Ni-Ti coil spring on the upper incisors as an anchor. Primary cultures were prepared from human periodontal ligament (HPDL) cells and subjected to tension and compression forces. In vivo: The SLPI level increased at 1d, 2d, and 6d after OTM by real time-PCR and Western blot. Immunoreactivity against SLPI was mainly found in the PDL and enhanced in the tension and compression side. In vitro: SLPI worked by acting on MAPK pathways including ERK and p38. SLPI mRNA was upregulated by tension or compression. SLPI siRNA treatment induced upregulation of IL-6 and IL-8 in HPDL cells during the tension applications. However, SLPI siRNA treatment induced the downregulation of IL-8 during compression application. These results suggest that SLPI in the PDL may be involved in inflammation control in OTM.
참고문헌 (38)
- Krishnan V, "On a path to unfolding the biological mechanisms of orthodontic tooth movement", J Dent Res, 88(7), pp.597~608 (2009)[DOI]
- Krishnan V, "Cellular, molecular, and tissue-level reactions to orthodontic force", Am J Orthod Dentofacial Orthop, 129(4), pp.469 e461~432 (2006)[DOI]
- Davidovitch Z, "Neurotransmitters, cytokines, and the control of alveolar bone remodeling in orthodontics", Dent Clin North Am, 32(3), pp.411~435 (1988)
- Kim SJ, "Cemental tear : Radioographic-clinical characteristics abd its predisposing factors", Korean J Oral Antomy, 40, pp.27~42 (2019)
- Melsen B, "Tissue reaction to orthodontic tooth movement--a new paradigm", Eur J Orthod, 23(6), pp.671~681 (2001)[DOI]
- Asano M, "IL-8 and MCP-1 induced by excessive orthodontic force mediates odontoclastogenesis in periodontal tissues", Oral Dis, 17(5), pp.489~498 (2011)[DOI]
- Silva TA, "Chemokines in oral inflammatory diseases : apical periodontitis and periodontal disease", J Dent Res, 86(4), pp.306~319 (2007)[DOI]
- Thammasitboon K, "Role of macrophages in LPS-induced osteoblast and PDL cell apoptosis", Bone, 38(6), pp.845~852 (2006)
- Kaku M, "VEGF and M-CSF levels in periodontal tissue during tooth movement", Biomed Res, 29(4), pp.181~187 (2008)[DOI]
- McComb JL, "Orthodontic treatment and isolated gingival recession : a review", Br J Orthod, 21(2), pp.151~159 (1994)[DOI]
- Abuabara A, "Biomechanical aspects of external root resorption in orthodontic therapy", Med Oral Patol Oral Cir Bucal, 12(8), pp.E610~613 (2007)
- Yamaguchi M, "Is inflammation a friend ot foe for orthodontic treatment? Inflammation in orthodontically induced inflammatory root resorption and accelerating tooth movement", Int J Mol Sci, 22(5), pp.2388~ (2021)[DOI]
- Lee SY, "SLPI in periodontal Ligament is not sleepy during biophysical force-induced tooth movement", J Clin Periodontol, 48(4), pp.528~540 (2021)[DOI]
- Natali AN, "Numerical analysis of tooth mobility : formulation of a non-linear constitutive law for the periodontal ligament", Dent Mater, 20(7), pp.623~629 (2004)[DOI]
- Wescott DC, "Osteogenic gene expression by human periodontal ligament cells under cyclic tension", J Dent Res, 86(12), pp.1212~1216 (2007)[DOI]
- Alhashimi N, "Chemokines are upregulated during orthodontic tooth movement", J Interferon Cytokine Res, 19(9), pp.1047~1052 (1999)[DOI]
- Ren Y, "Optimum force magnitude for orthodontic tooth movement : a systematic literature review", The Angle orthodontist, 73(1), pp.86~92 (2003)[DOI]
- Abe T, "Expression of the secretory leukoprotease inhibitor gene in epithelial cells", J Clin Invest, 87(6), pp.2207~2215 (1991)[DOI]
- Williams SE, "SLPI and elafin: one glove, many fingers", Clin Sci (Lond), 110(1), pp.21~35 (2006)[DOI]
- Angelov N, "Aberrant mucosal wound repair in the absence of secretory leukocyte protease inhibitor", Thromb Haemost, 92(2), pp.288~297 (2004)[DOI]
- Shugars DC, "Endogenous mucosal antiviral factors of the oral cavity", J Infect Dis, 179(Suppl 3), pp.S431~435 (1999)[DOI]
- Wiedow O, "Antileukoprotease in human skin : an antibiotic peptide constitutively produced by keratinocytes", Biochem Biophys Res Commun, 248(3), pp.904~909 (1998)[DOI]
- Barros SC, "Influence of secretory leukocyte protease inhibitor-based peptides on elastase activity and their incorporation in hyaluronic acid hydrogels for chronic wound therapy", Biopolymers, 98(6), pp.576~590 (2012)[DOI]
- Henriksen PA, "Adenoviral gene delivery of elafin and secretory leukocyte protease inhibitor attenuates NF-kappa B-dependent inflammatory responses of human endothelial cells and macrophages to atherogenic stimuli", J Immunol, 172(7), pp.4535~4544 (2004)[DOI]
- Jin FY, "Secretory leukocyte protease inhibitor : a macrophage product induced by and antagonistic to bacterial lipopolysaccharide", Cell, 88(3), pp.417~426 (1997)[DOI]
- Taggart CC, "Secretory leucoprotease inhibitor binds to NF-kappaB binding sites in monocytes and inhibits p65 binding", J Exp Med, 202(12), pp.1659~1668 (2005)[DOI]
- Hayashi N, "T-helper 17 cells mediate the osteo", odontoclastogenesis induced by excessive orthodontic forces, 2012, pp.18(4), 375~388
- Kwan Tat S, "IL-6, RANKL, TNF-alpha", IL-1 : interrelations in bone resorption pathophysiology, 2004, pp.15(1), 49~60
- 구승준, "Static tensional forces increase osteogenic gene expression in three-dimensional periodontal ligament cell culture", BMB reports, 42(7), pp.427~432 (2009), 한국생화학분자생물학회
- Lee YH, "Differential gene expression of periodontal ligament cells after loading of static compressive force", J Periodontol, 78(3), pp.446~452 (2007)[DOI]
- Lee SI, "Mechanical stress-activated immune response genes via Sirtuin 1 expression in human periodontal ligament cells", Clin Exp Immunol, 168(1), pp.113~124 (2012)[DOI]
- Brat DJ, "The role of interleukin-8 and its receptors in gliomagenesis and tumoral angiogenesis", Neuro Oncol, 7(2), pp.122~133 (2005)[DOI]
- Lattanzio L, "Role of IL-8 induced angiogenesis in uveal melanoma", Invest New Drugs, 31(5), pp.1107~1114 (2013)[DOI]
- Henneman S, "Mechanobiology of tooth movement", Eur J Orthod, 30(3), pp.299~306 (2008)[DOI]
- Greene CM, "Secretory leucoprotease inhibitor impairs Toll-like receptor 2-and 4-mediated responses in monocytic cells", Infect Immun, 72(6), pp.3684~3687 (2004)[DOI]
- McElvaney NG, "Modulation of airway inflammation in cystic fibrosis. In vivo suppression of interleukin-8levels on the respiratory epithelial surface by aerosolization of recombinant secretory leukoprotease inhibitor", J Clin Invest, 90(4), pp.1296~1301 (1992)[DOI]
- Zhu J, "Conversion of proepithelin to epithelins : roles of SLPI and elastase in host defense and wound repair", Cell, 111(6), pp.867~878 (2002)[DOI]
- Heinrich PC, "Interleukin-6and the acute phase response", Biochem J, 265(3), pp.621~636 (1990)[DOI]
