Biochemical Characteristics of Psychrotrophs Isolated
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Isolation of psychrotrophic lactic acid bacteria (LAB) from Chonggak kimchi was attempted in order to study characteristics of LAB growing at the low temperature. Among isolated strains, CL-1 showed exactly the same fermentation characteristics of Leuc. mesenteroides subsp. mesenteroides KCTC 3722 in tested carbohydrates except melezitose and the CL-1 showed very wide range temperature profiles (16C-28C) for growth comparing to other isolates. Its lowest temperature for growth was 16C which was much lower than other isolates. However all isolates couldn’t grow at 30C. In order to identify sudden inhibition of cell growth occurred at the transition temperature (16C → 14C), the CL-1 was tested for fermentation ability of hexoses and disaccharides at the lowered temperature. Among 5 tested hexoses, significant change was resulted from mannose fermentation. The CL-1 fermented mannose very well at 24C that gave a final pH 4.7 after 48 hr incubation but at 16C its final pH was only 5.7. This means that the CL-1 could not ferment mannose for its proper cell growth at 16C and inhibition of the cell growth was to begin. Similar tendency was found in fermentation of disaccharides at the lowered temperature. Among 6 tested disaccharides, inhibition of fermentation occurred only in melibiose. The CL-1 showed decreasing fermentation ability with the melibiose from pH 4.8 at 24C to pH 5.2 at 16 C. Fermentation of maltose, sucrose and trehalose was not affected by the lowered temperature at all. Thus, such growth inhibition of the psychrotrophs by lowered temperatures is related to occurrence of inability of fermenting the sugars and it leads to sudden growth inhibition of the psychrotrophic CL-1. From the test for bile salt tolerance, the CL-1 showed strong tolerance for glycocholate as much as Leuc. mesenteroides subsp. mesenteroides KCTC 3722 but both strains were strongly inhibited by 0.5% taurocholate. In case of taurocholate, the CL-1 could overcome the bile salt effect on cell growth with 0.2% taurocholate as incubation time goes by from 12 hr to 24 hr. The taurocholate tolerance of CL-1 was much better than that of Leuc. mesenteroides subsp. mesenteroides KCTC 3722.
Abstract
Isolation of psychrotrophic lactic acid bacteria (LAB) from Chonggak kimchi was attempted in order to study characteristics of LAB growing at the low temperature. Among isolated strains, CL-1 showed exactly the same fermentation characteristics of Leuc. mesenteroides subsp. mesenteroides KCTC 3722 in tested carbohydrates except melezitose and the CL-1 showed very wide range temperature profiles (16C-28C) for growth comparing to other isolates. Its lowest temperature for growth was 16C which was much lower than other isolates. However all isolates couldn’t grow at 30C. In order to identify sudden inhibition of cell growth occurred at the transition temperature (16C → 14C), the CL-1 was tested for fermentation ability of hexoses and disaccharides at the lowered temperature. Among 5 tested hexoses, significant change was resulted from mannose fermentation. The CL-1 fermented mannose very well at 24C that gave a final pH 4.7 after 48 hr incubation but at 16C its final pH was only 5.7. This means that the CL-1 could not ferment mannose for its proper cell growth at 16C and inhibition of the cell growth was to begin. Similar tendency was found in fermentation of disaccharides at the lowered temperature. Among 6 tested disaccharides, inhibition of fermentation occurred only in melibiose. The CL-1 showed decreasing fermentation ability with the melibiose from pH 4.8 at 24C to pH 5.2 at 16 C. Fermentation of maltose, sucrose and trehalose was not affected by the lowered temperature at all. Thus, such growth inhibition of the psychrotrophs by lowered temperatures is related to occurrence of inability of fermenting the sugars and it leads to sudden growth inhibition of the psychrotrophic CL-1. From the test for bile salt tolerance, the CL-1 showed strong tolerance for glycocholate as much as Leuc. mesenteroides subsp. mesenteroides KCTC 3722 but both strains were strongly inhibited by 0.5% taurocholate. In case of taurocholate, the CL-1 could overcome the bile salt effect on cell growth with 0.2% taurocholate as incubation time goes by from 12 hr to 24 hr. The taurocholate tolerance of CL-1 was much better than that of Leuc. mesenteroides subsp. mesenteroides KCTC 3722.
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