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NaCl Effect on Dextran Producing Leuconostoc mesenteroides CS-5 that Isolated from Chonggak Kimchi

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초록

Characteristics of Leuconostoc mesenteroides strains isolated from chonggak kimchi fermented in a refrigerator were studied in order to identify salinity nature. Among the isolates, a strain named CS-5 showed an optimal growth temperature ranged from 24℃ to 28℃. Interestingly the CS-5 was turned out to be a dextran producing strain. It could grow in the media containing 6% NaCl. At 5% NaCl the CS-5 revealed much better growth in media containing glucose or fructose as a monosaccharide than a strain of CL-1 that could not grow at all. For disaccharides (maltose, sucrose), the CS-5 showed strong growth nature at 6% salinity in which the CL-1 could not grow at all. This kind of salt tolerance of CS-5 also appeared in the media containing trehalose, melibiose or cellobiose that were known to be hard to ferment. Based on a test for dextran production, the CS-5 showed higher values of viscosity (0.153 Pa.Sn) in media containing 30% sucrose (w/v) comparing to the controls (0.01 Pa.Sn). Optimum fermentation time was determined between 48 hours and 60 hours for highest dextran production by Leuc. mesenteroides CS-5.

Abstract

Characteristics of Leuconostoc mesenteroides strains isolated from chonggak kimchi fermented in a refrigerator were studied in order to identify salinity nature. Among the isolates, a strain named CS-5 showed an optimal growth temperature ranged from 24℃ to 28℃. Interestingly the CS-5 was turned out to be a dextran producing strain. It could grow in the media containing 6% NaCl. At 5% NaCl the CS-5 revealed much better growth in media containing glucose or fructose as a monosaccharide than a strain of CL-1 that could not grow at all. For disaccharides (maltose, sucrose), the CS-5 showed strong growth nature at 6% salinity in which the CL-1 could not grow at all. This kind of salt tolerance of CS-5 also appeared in the media containing trehalose, melibiose or cellobiose that were known to be hard to ferment. Based on a test for dextran production, the CS-5 showed higher values of viscosity (0.153 Pa.Sn) in media containing 30% sucrose (w/v) comparing to the controls (0.01 Pa.Sn). Optimum fermentation time was determined between 48 hours and 60 hours for highest dextran production by Leuc. mesenteroides CS-5.

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