자궁경부 상피내 종양에서 인체 유두종바이러스의 검출

Abstract

Cervix carcinoma of the uterus is the most common cancer of the Korean women, but its early detection method is not popularized yet. Recent reports indicated human papilloma virus(HPV) as one of the major factors to the development of the cervical cancer. This study is perfomed to find out whether the epithelia of the uterine cervix were infected by HPV or not by using an in situ PCR method. None of the chronic nonspecific inflammation(CNI) case was positive for HPV, but 23.3% cases of cervical intraepithelial neoplasia(CIN) grade 1 were positive by immunohistochemistrry. But the positive rates by in situ PCR were different: 76.2% in CNI cases and 56.7% in CIN grade 1 cases. The positive cases of more than one HPV subtypes(mixed subtypes positive cases) which included a high risk group was 40 among the tota1 51 cases(80%) by in situ PCR method. This finding suggest that HPV positive cases have a possibility to progress to an early cancer. The most significant finding in this study was that CNI cases showed a high positive rate for HPV by in situ PCR method. This positive finding was usually detected in the koilocytes of cervical epithelium, although in 4 cases non-koilocytes were also positive. This study suggests that the DNA test for HPV should be carried for the early detection of the high-risk group of HPV infection. And these patients need follow-up study and regular examination; and the efforts would contribute to the reduction of the cervical cancers.