다이옥신-유도 간 독성에 대한 녹용추출물의 효과

Abstract

We have previously reported that intraperitoneal (i.p.) injection of Deer antler extract demonstrated preventive and therapeutic effects against testicular toxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in male rats. Forty rats (250±20g) were divided into 4 groups: normal controls (group 1) received vehic1e and saline; group 2 (single TCDD-treated) received TCDD (20 μg/kg, i.p.) and saline (p.o.); groups 3 and 4 received Deer antler extract from Deer antler (DA) at daily oral dose of 10 and 20 mg/kg, respectively, and vehicle (i.p.) for 6 wks from 1 wk after TCDD-exposure. Weights of kidney, spleen and testis of the TCDD-exposed rat were significantly decreased. Moderate swelling of hepatocytes, hyperchromatism, acidophilic cytoplasm and cytoplasmic vacuolation, single cell necrosis, small groups of degenerating hepatocytes surrounded by mononuclear cells and some loss of lobular architecture were observed in G2 animals. on the other hand, histopathological damage was moderately to markedly alleviated in groups 3 and 4, but pretreatment of Deer antler was more effective than the simultaneous treatment. From these results, we could understand the active compounds of Deer antler and further prove previously reported preventive and therapeutic effect of Deer antler against TCDD-induced toxicity in male rats.