겨우살이 Lectin의 간기능 영향

Abstract

In the present study, the author investigated changes in liver function caused by Viscum album lectin. The lectin was purified by 0.15 M NaCl extraction, amnonium sulfate precipitation, sepharose 4B affmity chromatography and gel filtration using sephadex 0-150 with plant material from Viscum album collected in Mt. Duk Yui. After 72 h of CCl4 iniection (in olive oil, 1:1, 2mg/kg) there was a significant increase in serum AST and AL T levels relative to the control group. However, treatment. of either Viscum album extracts and purified lectin offered noprotection against CCl4-induced hepatotoxicity. Histological observation basically supported the result obtained from serum enzyme assay. The livers of rats challenged with CCl4 produced a marked increase in cytoplasmic vacuoles and inflammatory cells in number, while the number of necrotic cells and swollen hepatocytes did not change significnatly. Rats administered olive oil alone did not have a change in the normal hepatic architecture. Histological observation of a liver section in rats treated 72 h with either Viscum album extract or purified lectin CCl4-induced liver damage showed numbers of cytoplasmic vaculoe and necrotic cells. The numbers of inflammatory cells were, however, increased markedly. The nùrmal hepatic architectural pattem was lost in a Hematoxylin-eosin stain. These results suggest the conc1usion that Viscum album lectin has a physiological effect of hepatotoxicity activator.