표면증강라만분석법을 이용한 항생제 내성 포도상 구균의 신속 동정
Rapid Detection of Methicillin Resistant Staphylococcus aureus Based on Surface Enhanced Raman Scattering
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Methicillin-resistant Staphylococcus aureus (MRSA) is one of the severe nosocomial infectious agents. The traditional diagnostic methods including biochemical test, antibiotic susceptibility test and PCR amplification are time consuming and require much work. The Surface enhanced Raman spectroscopy (SERS) biosensor is a rapid and powerful tool for analyzing the chemical composition within a single living cell. To identify the biochemical and genetic characterization of clinical MRSA, all isolates from patients were performed with VITEK2 gram positive (GP) bacterial identification and Antibiotic Susceptibility Testing (AST). Virulence genes of MRSA also were identified by DNA based PCR using specific primers. All isolates, which were placed on a gold coated nanochip, were analyzed by a confocal Raman microscopy system. All isolates were identified as S. aureus by biochemical tests. MRSA, which exhibited antibiotic resistance, demonstrated to be positive gene expression of both femA and mecA. Furthermore, Raman shift of S. aureus and MRSA (n=20) was perfectly distinguished by a confocal Raman microscopy system. This novel technique explained that a SERS based confocal Raman microscopy system can selectively isolate MRSA from non-MRSA. The study recommends the SERS technique as a rapid and sensitive method to detect antibiotic resistant S. aureus in a single cell level.
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the severe nosocomial infectious agents. The traditional diagnostic methods including biochemical test, antibiotic susceptibility test and PCR amplification are time consuming and require much work. The Surface enhanced Raman spectroscopy (SERS) biosensor is a rapid and powerful tool for analyzing the chemical composition within a single living cell. To identify the biochemical and genetic characterization of clinical MRSA, all isolates from patients were performed with VITEK2 gram positive (GP) bacterial identification and Antibiotic Susceptibility Testing (AST). Virulence genes of MRSA also were identified by DNA based PCR using specific primers. All isolates, which were placed on a gold coated nanochip, were analyzed by a confocal Raman microscopy system. All isolates were identified as S. aureus by biochemical tests. MRSA, which exhibited antibiotic resistance, demonstrated to be positive gene expression of both femA and mecA. Furthermore, Raman shift of S. aureus and MRSA (n=20) was perfectly distinguished by a confocal Raman microscopy system. This novel technique explained that a SERS based confocal Raman microscopy system can selectively isolate MRSA from non-MRSA. The study recommends the SERS technique as a rapid and sensitive method to detect antibiotic resistant S. aureus in a single cell level.
참고문헌 (12)
- An JH, "Surface-enhanced Raman scattering of dopamine on self-assembled gold nanoparticles", J Nanosci Nanotechnol, 11(5), pp.4424~4429 (2011)
- Barski P, "Rapid assay for detection of methicillin-resistant Staphylococcus aureus using multiplex PCR", Mol Cell Probes, 10(6), pp.471~475 (1996)
- Castellano MJ, "Antimicrobial susceptibility and polyclonal dissemination of Staphylococcus aureus strains", Rev Chilena Infectol, 31(2), pp.165~172 (2014)
- Champion A, "Inzana TJ. Prevalence and characterization of methicillin-resistant", Ann Clin Microbiol Antimicrob, 13(1), pp.33~ (2014)
- El-Said WA, "Analysis of intracellular state based on controlled 3D nanostructures mediated surface enhanced Ramanscattering", PLoS One, 6(2), pp.15836~ (2011)
- Lin HY, "On-line SERS Detection of Single Bacterium Using Novel SERS Nanoprobes and A Microfluidic Dielectrophoresis Device", Small, 10(22), pp.4700~4710 (2014)
- Liu TT, "A high speed detection platform based on surface-enhanced Raman scattering for monitoring antibiotic-inducedchemical changes in bacteria cell wall", PLoS One, 4(5), pp.e5470~ (2009)
- Lu X, "Detecting and tracking nosocomial methicillin-resistant Staphylococcus aureus using a microfluidic SERS biosensor", Anal Chem, 85(4), pp.2320~2327 (2013)
- McElvania TeKippe E, "Evaluation of the Bruker Biotyper and VITEK MS MALDI-TOF MS systems for the identification of unusual and", or difficult-to-identify microorganisms isolated from clinical specimens, 2014, pp.33(12), 2163~2171
- Nam HM, "Antimicrobial susceptibility of Staphylococcus aureus and characterization of methicillin-resistant Staphylococcus aureus isolated from bovine mastitis in Korea", Foodborne Pathog Dis, 8(2), pp.231~238 (2011)
- Park SY, "Clinical impact of methicillin-resistant Staphylococcus aureus bacteremia based on propensity scores", Infection, 39(2), pp.141~147 (2011)
- Vandenbroucke-Grauls CM, "Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR", J Clin Microbiol, 34(6), pp.1599~ (1996)