라다클로린으로 매개된 광역학치료에 의한 백색 캔디다 바이오필름의 비활성
Inactivation of Candida albicans Biofilm by Radachlorin-Mediated Photodynamic Therapy
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The purpose of this study was to evaluate the in-vitro efficacy of PDT using red light emitting diode (LED) with Radachlorin for biofilm inhibition of clinical Candida albicans isolates. The suspensions containing C. albicans at 9×108 CFU/mL were prepared on yeast nitrogen base containing 5% glucose. The biofilm formation was grown for 3 h after seeding suspensions each 100 ul on a 96-well plate and then supernatant was discarded. Each well was treated with 0.39 g/mL from 50 g/mL concentrations of Radachlorin on adherent biofilm. After a 30-minute incubation, light was irradiated for 30, 60, or 90 minutes using the following light source of wavelength 630 nm LED, at energy densities of 14, 29, and 43 J/cm2. Afterwards, all supernatant was removed and dried. Adherent cells were stained with safranin O and dried. The cell viability was measured using a microplate reader at 490 nm. Also, a fluorescent signal on C. albicans was observed by saturation of a photosensitizer. In conclusion, a significant inhibition of 72.5% was observed to C. albicans on biofilm at the Radachlorin dose of 50 g/mL with 630 nm LED. The Photosensitizer (Radachlorin) was adequate at 30 minuttes for C. albicans. Overall, the results showed that inhibition of biofilm formation was Radachlorine dose-dependent. The results suggest that PDT, using Radachlorin with 630 nm LED, is able to decrease biofilm formation of C. albicans.
Abstract
The purpose of this study was to evaluate the in-vitro efficacy of PDT using red light emitting diode (LED) with Radachlorin for biofilm inhibition of clinical Candida albicans isolates. The suspensions containing C. albicans at 9×108 CFU/mL were prepared on yeast nitrogen base containing 5% glucose. The biofilm formation was grown for 3 h after seeding suspensions each 100 ul on a 96-well plate and then supernatant was discarded. Each well was treated with 0.39 g/mL from 50 g/mL concentrations of Radachlorin on adherent biofilm. After a 30-minute incubation, light was irradiated for 30, 60, or 90 minutes using the following light source of wavelength 630 nm LED, at energy densities of 14, 29, and 43 J/cm2. Afterwards, all supernatant was removed and dried. Adherent cells were stained with safranin O and dried. The cell viability was measured using a microplate reader at 490 nm. Also, a fluorescent signal on C. albicans was observed by saturation of a photosensitizer. In conclusion, a significant inhibition of 72.5% was observed to C. albicans on biofilm at the Radachlorin dose of 50 g/mL with 630 nm LED. The Photosensitizer (Radachlorin) was adequate at 30 minuttes for C. albicans. Overall, the results showed that inhibition of biofilm formation was Radachlorine dose-dependent. The results suggest that PDT, using Radachlorin with 630 nm LED, is able to decrease biofilm formation of C. albicans.
참고문헌 (25)
- Blankenship JR, "How to build a biofilm: a fungal perspective", Curr. Opin. Microbiol, 9, pp.588~594 (2006)
- Dai T, "Blue dye and red light, a dynamic combination for prophylaxis and treatment of cutaneous Candida albicans infections in mice", Antimicrob Agents Chemother, 55, pp.5710~5717 (2011)
- Demidova TN, "Effect of cell-photosensitizer binding and cell density on microbial photoinactivation", Antimicrob Agents Chemother, 49, pp.2329~2335 (2005)
- Douglas LJ, "Medical importance of biofilms in Candida infections", Rev Iberoam Micol, 19, pp.139~143 (2002)
- Dovigo LN, "Photodynamic inactivation of four Candida species induced by photogem", Braz J Microbiol, 41, pp.42~49 (2010)
- Giroldo LM, "Photodynamic antimicrobial chemotherapy (PACT)with methylene blue increases membrane permeability in Candida albicans", Lasers Med Sci, 24, pp.109~112 (2009)
- Hawser SP, "Biofilm formation by Candida species on the surface of catheter materials in vitro", Infect Immun, 62, pp.915~921 (1994)
- Heilmann C, "Characterization of Tn917 insertion mutants of Staphylococcus epidermidis affected in biofilm formation", Infect Immun, 64, pp.277~282 (1996)
- 권필승, "Moraxella catarrhalis의 광역학적 비활성화", 대한임상검사과학회지, 39(1), pp.19~24 (2007), 대한임상검사과학회
- Imlay JA, "Pathways of oxidative damage", Annu Rev Microbiol, 57, pp.395~418 (2003)
- Junqueira JC, "Photodynamic inactivation of biofilms formed by Candida spp., Trichosporon mucoides, and Kodamaea ohmeri by cationic nanoemulsion of zinc 2,9,16,23-tetrakis(phenylthio)-29H, 31H-phthalocyanine (ZnPc)", Lasers Med Sci, 27, pp.1205~1212 (2012)
- 권필승, "반코마이신 내성 장구균에 대한 광에너지 요법의 효과", 대한임상검사과학회지, 43(3), pp.124~132 (2011), 대한임상검사과학회
- 권필승, "여드름균에 대한 청색 발광다이오드와 포토프린과 라다클로린을 이용한 광역학치료의 항균효과", 대한임상검사과학회지, 47(1), pp.6~10 (2015), 대한임상검사과학회[DOI]
- Lam M, "Photodynamic therapy with Pc 4 induces apoptosis of Candida albicans", Photochem Photobiol, 87, pp.904~909 (2011)
- Mayer FL, "Candida albicans pathogenicity mechanisms", Virulence, 4, pp.119~128 (2013)
- Morgan J, "Excess mortality, hospital stay, and cost due to candidemia: a case–control study using data from population-based candidemia surveillance", Infect Control Hosp Epidemiol, 26, pp.540~547 (2005)
- Odds FC, "Fungal virulence studies come of age", Genome Biol, 2, pp.1009.1~1009.4 (2001)
- Oliver BG, "Tetracycline alters drug susceptibility in Candida albicans and other pathogenic fungi", Microbiology, 154, pp.960~970 (2008)
- Prates RA, "Influence of multidrug efflux systems on methylene blue-mediated photodynamic inactivation of Candida albicans", J Antimicrob Chemother, 66, pp.1525~1532 (2011)
- Ramage G, "Candida biofilms on implanted biomaterials: a clinically significant problem", FEMS Yeast Res, 6, pp.979~986 (2006)
- Ramage G, "Characteristics of biofilm formation by Candida albicans", Rev Iberoam Micol, 18, pp.163~170 (2001)
- Rosseti IS, "Photodynamic antimicrobial chemotherapy (PACT) inhibits biofilm formation by Candida albicans, increasing both ROS production and membrane permeability", Laser Med Sci., 29, pp.1059~1064 (2014)
- Shapiro RS, "Regulatory circuitry governing fungal development, drug resistance, and disease", Microbiol Mol Biol Rev, 75, pp.213~267 (2011)
- Thompson DS, "Coevolution of morphology and virulence in Candida species", Eukaryot. Cell, 10, pp.1173~1182 (2011)
- Vera DM, "Strategies to potentiate antimicrobial photoinactivation by overcoming resistant phenotypes", Photochem Photobiol, 88, pp.499~511 (2012)