Evaluation of Analyzer and Measurement Conditions of Blood Ammonia
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Ammonia is very toxic, and causes neuronal damage via excitotoxicity, oxidative stress, and inflammation. Because the liver is the primary organ for ammonia metabolism, compromised liver function can result from inborn errors of metabolism. Measurement of blood ammonia has some limitations. Recently, several laboratories examined possible concurrent increases in plasma ammonia. However, the collection, handling, storage, and analysis of blood samples are all potential sources of error. For evaluation of rapidity and reliability of measurement of blood ammonia, the DRI-CHEM 100 (Fuji Film Co., Japan) and COBAS 8000 (Roche Diagnostic Ltd., Switzerland) analyzer were used for analysis of ammonia level values. The results of this study detected a high correlation between analyzer. Therefore, one-step measurement was suitable for ammonia analysis. After sampling of the ammonia in the time slot for measurement an increase to 46.5, 57.4, and 79.0 (μg/dL) was observed at 30, 90, and 180 minutes. In addition, specific capacity of the ammonia, 7, 10, and 13 (μL), was measured as 39, 46, and 43 (μg/dL), respectively, and the FDC-100 analyzer was more effective in 10 μL (p＜0.001). In conclusion, the evaluated analysis may offer useful information for clinical application.
Abstract
Ammonia is very toxic, and causes neuronal damage via excitotoxicity, oxidative stress, and inflammation. Because the liver is the primary organ for ammonia metabolism, compromised liver function can result from inborn errors of metabolism. Measurement of blood ammonia has some limitations. Recently, several laboratories examined possible concurrent increases in plasma ammonia. However, the collection, handling, storage, and analysis of blood samples are all potential sources of error. For evaluation of rapidity and reliability of measurement of blood ammonia, the DRI-CHEM 100 (Fuji Film Co., Japan) and COBAS 8000 (Roche Diagnostic Ltd., Switzerland) analyzer were used for analysis of ammonia level values. The results of this study detected a high correlation between analyzer. Therefore, one-step measurement was suitable for ammonia analysis. After sampling of the ammonia in the time slot for measurement an increase to 46.5, 57.4, and 79.0 (μg/dL) was observed at 30, 90, and 180 minutes. In addition, specific capacity of the ammonia, 7, 10, and 13 (μL), was measured as 39, 46, and 43 (μg/dL), respectively, and the FDC-100 analyzer was more effective in 10 μL (p＜0.001). In conclusion, the evaluated analysis may offer useful information for clinical application.
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