논문KCI 등재
세라미드는 신장 상피세포에서 ERK의존성 미토콘드리아 세포자멸사 경로를 통해 사포사멸을 유도
Ceramide Induces Cell Death through an ERK-dependent Mitochondrial Apoptotic Pathway in Renal Epithelial Cells
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
주제분류기타자연과학
초록
.
Abstract
Ceramide induces cell death in a variety of cell types however, the underlying molecular mechanisms related to renal epithelial cells remain unclear. The present study was undertaken to determine the role of extracellular signal-regulated protein kinase (ERK) in ceramide-induced cell death in renal epithelial cells. An established renal proximal tubular cell line of opossum kidney (OK) cells was used for this research. Ceramide induced apoptotic cell death in these cells. Western blot analysis showed that ceramide induced activation of ERK. The ERK activation and cell death induced by ceramide were prevented by the ERK inhibitor PD98059. Ceramide caused cytochrome C release from mitochondria into the cytosol as well as activation of caspase-3. Both effects were prevented by PD98059. The ceramide-induced cell death was also prevented by a caspase inhibitor. These results suggest that ceramide induces cell death through an ERK-dependent mitochondrial apoptotic pathway in OK cells.
참고문헌 (30)
- Aikawa R, "Oxidative stress activates extracellular signal-regulated kinases through Src and Ras in cultured cardiac myocytes of neonatal rats", J Clin Invest, 100, pp.1813~1821 (1997)
- Alessi DR, "PD98059 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo", J Biol Chem, 270, pp.27489~27494 (1995)
- Bhat NR, "Hydrogen peroxide activation of multiple mitogen-activated protein kinases in an oligodendrocyte cell line: role of extracellular signalregulated kinase in hydrogen peroxide-induced cell death", J Neurochem, 72, pp.112~119 (1999)
- Blazquez C, "De novosynthesized ceramide signals apoptosis in astrocytesvia extracellular signal-regulated kinase", Faseb J, 14, pp.2315~2322 (2000)
- Chandra D, "Early mitochondrial activation and cytochrome C up-regulation during apoptosis", J Biol.Chem, 52, pp.50842~50854 (2002)
- Choi BK, "Role of ERK activation in cisplatin-induced apoptosis in A172human glioma cells", Neuro Toxicology, 25, pp.915~924 (2004)
- Cobb MH., "MAP kinase pathways", Prog Biophys Mol Biol, 71, pp.479~500 (1999)
- Cohen G. M., "Caspases : the executioners of apoptosis", Biochem J, 326, pp.1~16 (1997)
- Cross TG, "Serine", threonine protein kinases and apoptosis, 2000, pp.256, 34~41
- Cuvillier O, "Sphingolipid-mediated apoptotic signaling pathways", J Soc Biol, 197, pp.217~221 (2003)
- Dbaibo GS, "Signal transduction and the regulation of apoptosis : roles of ceramide", Apoptosis, 3, pp.317~334 (1998)
- Denizot F, "Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability", J Immunol, 89, pp.271~277 (1986)
- Dudley DT, "A synthetic inhibitor of the mitogen-activated protein kinase cascade", Proc Natl Acad Sci U.S.A, 92, pp.7686~7689 (1995)
- Guyton KZ, "Activation of mitogen-activated protein kinase by H2O2. Role in cell survival following oxidant injury", J Biol Chem, 271, pp.4138~4142 (1996)
- Jimenez LA, "Role of extracellular signal-regulated protein kinases in apoptosis by asbestos and H2O2", Am J Physiol, 273, pp.L1029~L1035 (1997)
- Kim SH, "Activation of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) induces cell death through MAPK-dependent mechanism in osteoblastic cells", Toxicol Appl Pharmacol, 215, pp.198~207 (2006)
- Kim WH, "Ceramide induces non-apoptotic cell death in human glioma cells", Neurochem Res, 30, pp.969~979 (2005)
- Kim YK, "Role of ERK activation in cisplatininduced apoptosis in OK renal epithelial cells", J Appl Toxicol, 25, pp.374~382 (2005)
- Kolesnick RN, "Regulation of ceramide production and apoptosis", Annu Rev Physiol, 60, pp.643~665 (1998)
- Lee WC, "Role of ERK in hydrogen peroxide-induced cell death of human glioma cells", Neurochem Res, 30, pp.263~270 (2005)
- Luberto C, "Sphingolipid metabolism in the regulation of bioactive molecules", Lipids, 34(Suppl), pp.S5~S11 (1999)
- Nowak G., "PKC-a and ERK1", 2 mediate mitochondrial dysfunction, decreases in active Na+ transport, and cisplatin-induced apoptosis in renal cells, 2002, pp.277, 43377~43388
- Oh HL, "Role of MAPK in ceramide-induced cell death in primary cultured astrocytes from mouse embryonic brain", Neurotoxicology, 27, pp.31~38 (2006)
- Pearson G, "Mitogenactivated protein(MAP)kinase pathways : regulation and physiological functions", Endocr Rev, 22, pp.153~183 (2001)
- Pena LA, "Stress-induced apoptosis and the sphingomyelin pathway", Biochem Pharmacol, 53, pp.615~621 (1997)
- Pettus BJ, "Ceramide in apoptosis : an overview and current perspectives", Biochem Biophys Acta, 1585, pp.114~125 (2002)
- Seefelder W, "Induction of apoptosis in cultured human proximal tubule cells by fumonisins and fumonisin metabolites", Toxicol Appl Pharmacol, 192, pp.146~153 (2003)
- Wang X, "Requirement for ERK activation in cisplatin-induced apoptosis", J Biol Chem, 275, pp.39435~39443 (2000)
- WoessmannW, "Ras-mediated activation of ERK by cisplatin induces cell death independently of p53 in osteosarcoma and neuroblastoma cell lines", Cancer Chemother. Pharmacol, 50, pp.397~404 (2002)
- Xia Z, "Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis", Science, 270, pp.1326~1331 (1995)
관련 논문AI추천
세라마이드에 의한 신경세포사멸에서 p53 조절을 통한 사립체 손상에 관한 연구김경용, 김성수, 노유헌, 윤지영, 이도연, 이원복, 이현정, 정윤희, 한인숙대한체질인류학회 · 해부·생물인류학2006 · 제19권 제1호Mercuric Chloride에 의한 MDCK 세포의 세포사멸권근상, 염정호, 이주형대한예방의학회 · 예방의학회지2006 · 제39권 제3호콜레스테롤 산화 생성물 7-Ketocholesterol에 의한 세포자멸사에 미치는 Extracellular Signal-Regulated Kinase 억제의 효과김두응, 이선화, 이정수, 한정호, 황정연대한신경과학회 · 대한신경과학회지2011 · 제29권 제4호가시오갈피의 세라마이드에 의한 신경세포 어포토시스 억제김경용, 김대성, 김성수, 손동섭, 이도연, 이원복대한체질인류학회 · 해부·생물인류학2003 · 제16권 제4호실험적 급성뇨세관괴사에서 세포자멸사와 세포증식능에 관한 연구김완섭, 노정우, 박문향대한병리학회 · Journal of Pathology and Translational Medicine2003 · 제37권 제1호인간망막 내피세포주에서 고농도 포도당이 caspase-3 경로를 통해 세포자연사 유도고은경, 서은선, 이종빈, 채수철한국환경생물학회 · 환경생물2009 · 제27권 제1호