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Inhibition of Cervical Cancer Cell Growth by Gene Silencing of HPV16 E6 Induced by Short-interfering RNA

박상묵|김윤식
대한임상검사과학회 (Korean Society for Clinical Laboratory Science)|2011.09|대한임상검사과학회지 (Korean Journal of Clinical Laboratory Science)|제43권 제3호|pp. 89-97

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Abstract

The Human Papilloma Virus (HPV) infection has been strongly associated with pathogenesis of uterine cervix carcinoma. HPV type 16, a causative agent of uterine cervix carcinoma, encodes the E6 and E7 oncogenes, expression of which is pivotal for malignant transformation and maintenance of malignant phenotypes. To develop a gene therapy for HPV-related carcinoma, We investigated the effect of E6 short-interfering RNA (E6 siRNA) on the expression of this oncogene and on the growth of HPV 16-related uterine cervix carcinoma cells. SiHa cells, a uterine cervix carcinoma cell line, which contain a single copy of HPV 16 integrated in the chromosome and express the E6 and E7 oncogenes. Before 24 hr of transfection, cells were seeded and transfected with control plasmid or E6 siRNA-expressing plasmid. The mRNA was analysed by reverse transcriptase polymerase chain reaction (RT- PCR). The cell growth rate was investigated by MTT method. The E6 mRNA level in SiHa cells was decreased in HPV 16 E6 siRNA-expression vector transfected cells and a decrease in the growth of these cells was also observed. From these results. it is evident that E6 siRNA played a role in suppression of growth of SiHa cells and has a fair chance as a candidate for gene specific therapy for HPV related uterine cervix carcinoma.

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관련 논문AI추천

SiHa 자궁경부암 세포주에서 인유두종바이러스 16형 E6/E7 siRNA에 의한 세포성장 억제 비교김용욱, 김태응, 노덕영, 박병준, 박세현, 배수미, 안웅식, 정재근대한산부인과학회 · Obstetrics & Gynecology Science2010 · 제53권 제01호The Role of HPV E6 and E7 Oncoproteins in HPV-associated Cervical CarcinogenesisEun-Kyoung Yim, Jong-Sup Park대한암학회 · Cancer Research and Treatment2005 · 제37권 제6호Cell-Specific Growth Inhibition of Human Cervical Cancer Cell by Recombinant Adenovirus p53 in vitro and in vivoChong Kook Kim, Jin Young Yoo, Joo Hee Yoon, Joon Mo Lee, Sang Lyun Nam, Su Mi Bae, Sung Eun Namkoong, Woong Shick Ahn, Yong-Wan Kim, Yong Wook Kim, Young-Seok Seo대한암학회 · Cancer Research and Treatment2003 · 제35권 제3호Cell Cycle Regulatory Protein Expression Profiles by Adenovirus p53 Infection in Human Papilloma Virus-associated Cervical Cancer CellsByoung-Don Han, Chong-Kook Kim, Do Kang Kim, Dong-Chun Park, Eun-Kyung Park, Soo-Young Hur, Su-Mi Bae, Sun-Young Kwak, Woong-Shick Ahn, Yong-Seok Lee, Yong-Wook Kim, Young- Joo Lee대한암학회 · Cancer Research and Treatment2006 · 제38권 제3호Human Papillomavirus Type 16 E5 Protein as a Therapeutic Target김상우, 양주성연세대학교의과대학 · Yonsei Medical Journal2006 · 제47권 제1호Aberrant Cell Cycle Regulation in Cervical Carcinoma김영태, Min Zhao연세대학교의과대학 · Yonsei Medical Journal2005 · 제46권 제5호

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