Nested PCR을 이용하여 조직으로부터 Mycobacterium tuberculosis Complex 신속검출
Rapid Detection of Mycobacterium tuberculosis Complex in Tissues by Using the Nested PCR
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Due to the increase in incidence of infection of Mycobacterium tuberculosis complex (MTC), it is imperative that a rapid diagnosis accompanies the handling of MTC. This is due to the three to eight weeks it takes to culture Mycobacteria, and the lack of sensitivity of microscopic examination of AFB. Recently, nested PCR has been used to detect and diagnose mycobacteria. It is especially useful in complementing diagnosis by histological extra pulmonary. After culturing all the specimens and practicing the nested PCR, we did comparison analysis between nested PCR and culture. There were 76 specimens, 31 of which were positive. Of the 31 positive specimens in culturing, only 22 were positive in nested PCR. Of the 45 negative specimens, 36 were negative in nested PCR. As a result, Sensitivity was 71% and specificity was 80%. Furthermore, the positive predictive value was 71% and negative predictive value was 80%. These results indicate that nested PCR based techniques are sensitive, specific, and rapid methods for the detection of MTC.
Abstract
Due to the increase in incidence of infection of Mycobacterium tuberculosis complex (MTC), it is imperative that a rapid diagnosis accompanies the handling of MTC. This is due to the three to eight weeks it takes to culture Mycobacteria, and the lack of sensitivity of microscopic examination of AFB. Recently, nested PCR has been used to detect and diagnose mycobacteria. It is especially useful in complementing diagnosis by histological extra pulmonary. After culturing all the specimens and practicing the nested PCR, we did comparison analysis between nested PCR and culture. There were 76 specimens, 31 of which were positive. Of the 31 positive specimens in culturing, only 22 were positive in nested PCR. Of the 45 negative specimens, 36 were negative in nested PCR. As a result, Sensitivity was 71% and specificity was 80%. Furthermore, the positive predictive value was 71% and negative predictive value was 80%. These results indicate that nested PCR based techniques are sensitive, specific, and rapid methods for the detection of MTC.
참고문헌 (21)
- Boddinghaus B, "Detection and identification of mycobacteria by amplification of rRNA", J Clin Microbiol, 28, pp.1751~1759 (1990)
- Brisson-Noel A, "Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation", Lancet, 338, pp.364~366 (1991)
- Brosch R, "A new evolutionary scenario for the Mycobacterium tuberculosis complex", Proc Natl Acad Sci, 99, pp.3684~3689 (2002)
- Chan CM, "Single-tube nested PCR in the diagnosis of tuberculosis", J Clin Pathol, 49, pp.290~294 (1996)
- 최우순, "신선조직 검체에서 결핵균 검출을 위한 자동화 중합효소연쇄반응 검사의 유용성", Tuberculosis and Respiratory Diseases, 61(1), pp.54~59 (2006), 대한결핵및호흡기학회
- De Wit D, "Direct detection of Mycobacterium tuberculosis in clinical specimens by DNA amplification", J Clin Microbiol, 28, pp.2437~2441 (1990)
- Cristina P., "Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR", Bra J Micro, 45, pp.6333~6640 (2014)
- Eisenach KD, "PCR amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis", J Infect Dis, 28, pp.1204~1213 (1990)
- Frevel, K L schafer, "PCR based detection of mycobacteria in paraffin wax embedded material routinely processed for morphological examination", J Clin Patholl, 52, pp.283~288 (1999)
- WHO report on the tuberculosis epidemic 1996, "Groups at risk", World Health Organization, ~ (1996)
- Hance A, "Detection and identification of Mycobacteria by amplification of mycobacterial DNA", Mol Microbiol, 3, pp.843~849 (1989)
- Hermans PW, "Specific detection of Mycobacterium tuberculosis complex strains by polymerase chain reaction", J Clin Microbiol, 28, pp.1204~1213 (1990)
- 장형석, "객담을 이용한 Mycobacteria의 검출과 중합효소 연쇄반응의 민감성 비교", 대한임상검사과학회지, 47(2), pp.83~89 (2015), 대한임상검사과학회[DOI]
- Jonas V, "Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA", J Clin Microbiol, 31, pp.2410~2416 (1993)
- Osaki M, "Detection of mycobacterial DNA in formalin-fixed, paraffin-embedded tissue specimens by duplex polymerase chain reaction: application to histopathologic diagnosis", Mod Pathol, 10, pp.78~83 (1997)
- Savic B, "Evaluation of polymerase chain reaction, tuberculostearic acid analysis, and direct microscopy for the detection of Mycobacterium tuberculosis in sputum", J Infect Dis, 166, pp.1177~1180 (1992)
- Shinnick TM, "The 65-kilodalton antigen of Mycobacterium tuberculosis", J Bacteriol, 169, pp.1080~1088 (1987)
- Thierry D, "Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis", J Clin Microbiol, 28, pp.2668~2673 (1990)
- Totsch M, "Diagnostic value of different PCR assay for the detection of mycobacterial DNA in granulomatous lymphadenopathy", J Pathol, 178, pp.221~226 (1996)
- "Treatment of tuberculosis guidelines for national programmers" (1995), World Health Organization
- Vago L, "Nested Polymerase chain reaction for Mycobacterium tuberculosis IS6110 sequence on formalin-fixed paraffin-embedded tissues with granulomatous diseases for rapid diagnosis of tuberculosis", Am J Clin Pathol, 109, pp.411~415 (1998)