임상검체에서 분리된 Aspergillus Species의 연구
Study of Aspergillus Species from Clinical Specimen Isolate
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초록
Aspergillus는 가장 흔하게 감염을 일으키는 기회 감염균이다. 검사실에서 Aspergillus의 동정은 대부분 형태학적으로 이루어진다. 최근에는 형태학적으로 동정이 안 되거나, 속 수준까지만 동정이 되는 까다로운 균들 동정하기 위하여 분자유전학적인 방법들이제안되고 있다. 임상검체에서 분리한 475건의 Aspergillus는Aspergillus fumigatus 257주(54.1%), A. niger 101주(21.3%), A. flavus 43주(9.1%), A. terreus 29주(6.1%), Aspergillus nidulans 2주(0.4%), 그리고 Aspergillus clavatus 1주(0.2%), 그리고Aspergillus species는 42주(8.8%)이었다. 미동정 균주와 종 구분을 위한 11건의 염기서열분석에서는 Aspergillus fumigatus 5주, Aspergillus falvus 1주, Aspergillus terreus 1주, Aspergillus lentulus 1주 동정되었다. Aspergillus versicolor 2주, 그리고 1주는 Emericella parvathecia로 동정되었다. Aspergillus의 92.2% 는 형태학적으로 동정이 가능하였고, 8.8%는 종 수준의 동정이 불가능하였다. 종 수준의 동정을 하기 위해서는 ITS와 D1D2를 이용한 염기서열분석이 유용한 것으로 사료된다.
Abstract
Aspergillus is the most common opportunistic fungus causing infection. Aspergillus is the mostmorphologically identified in the laboratory. Recently, molecular genetic methods have beenproposed for identification of fungi that unidentified morphologically or identified genus level. Of475 cases of Aspergillus isolated from clinical specimens, there were Aspergillus fumigatus 257(54.1%), A. niger 101 (21.3%), A. flavus 43 (9.1%), A. terreus 29 (6.1%), Aspergillus nidulans 2(0.4%), Aspergillus clavatus 1 (0.2%), and the Aspergillus species 42 (8.8%). Eleven cases ofunidentified or identified at the genus level included Aspergillus fumigatus 5, Aspergillus falvus 1,Aspergillus terreus 1, and Aspergillus lentulus 1 was identified in the sequencing of the strain level. It was identified as Aspergillus versicolor 2, and Emericella parvathecia 1. 92.2% of Aspergilluswas identified as a possible morphological, 8.8% could not be identified at the species level. Sequence-based molecular analysis using the ITS and D1D2 is considered useful for identificationof the species level.
참고문헌 (19)
- Lee NY, "Clinical cases in medical mycology", pp.133~172 (2015), Panmun education
- Larone DH, "Medically important fungi: A guide to identification" (2011), ASM Press
- Koulenti D, "Approach to invasive pulmonary aspergillosis in critically ill patients", Curr Opin Infect Dis, 27, pp.174~183 (2014)
- 김영권, "객담에서 분리한 Aspergillus 속의 RAPD를 이용한 분자생물학적 동정의 유용성", 대한임상검사과학회지, 41(4), pp.158~166 (2009), 대한임상검사과학회
- Ki HK, "Invasive aspergillosis after solid organ transplantation", 31, pp.500~505 (1999)
- Krishnan S, "Aspergillus flavus : an emerging non-fumigatus Aspergillus species of significance", Mycoses, 52, pp.206~222 (2009)
- Perfect JR, "Fungal diagnosis: how do we do it and can we do better?", Curr Med Res Opin, 29(Suppl 4), pp.3~11 (2013)
- 장자현, "Identification of Clinical Mold Isolates by Sequence Analysis of the Internal Transcribed Spacer Region, Ribosomal Large-Subunit D1", D2, and β-Tubulin, 2012, pp.32(2), 126~132[DOI]
- 허민석, "Molecular Identification and Amphotericin B Susceptibility Testing of Clinical Isolates of Aspergillus From 11 Hospitals in Korea", Annals of Laboratory Medicine, 35(6), pp.602~610 (2015), 대한진단검사의학회
- Dagenais TR, "Pathogenesis of Aspergillus fumigatus in invasive aspergillosis", Clin Microbiol Rev, 22, pp.447~465 (2009)
- Oda M, "Calcium oxalate crystal deposition in a patient with aspergilloma due to Aspergillus niger", J Thorac Dis, 5, pp.E174~E178 (2013)
- Arendrup MC, "Update on antifungal resistance in Aspergillus and Candida", Clin Microbiol Infect, 20, pp.S42~S48 (2014)
- Hachem R, "Invasive aspergillosis caused by Aspergillus terreus : an emerging opportunistic infection with poor outcome independent of azole therapy", J Antimicrob Chemother, 69, pp.3148~3155 (2014)
- Pastor FJ, "Treatment of Aspergillus terreus infections : A clinical problem not yet resolved", Int J Antimicrob Agents, 44, pp.281~289 (2014)
- Henriet SS, "Aspergillus nidulans and chronic granulomatous disease : a unique host-pathogen interaction", J Infect Dis, 206, pp.1128~1137 (2012)
- Opal SM, "Aspergillus clavatus endocarditis involving a normal aortic valve following coronary artery surgery", Rev Infect Dis, 8, pp.781~785 (1986)
- 허희재, "A Case of Misidentification of Aspergillus versicolor Complex as Scopulariopsis Species Isolated from a Homograft", Annals of Clinical Microbiology, 16(2), pp.105~109 (2013), 대한임상미생물학회[DOI]
- Summerbell RC, "Microcoding : the second step in DNA barcoding", Philos Trans R Soc Lond B Biol Sci, 360, pp.1897~1890 (2005)
- Zohri AA, "Progesterone transformation as a biochemical aid in classification of the genus Emericella", Folia Microbiol(Praha), 45(5), pp.391~396 (2000)