Pulsed Electromagnetic Field Enhances Doxorubicin-induced Reduction in the Viability of MCF-7 Breast Cancer Cells
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
A pulsed electromagnetic field (PEMF) enhances the efficacy of several anticancer drugs. Doxorubicin (DOX) is an anticancer agent used to treat various malignancies, including breast cancer. This study examined whether a PEMF increases the anticancer effect of DOX on MCF-7 human breast cancer cells and elucidated the underlying mechanisms affected by PEMF stimulation in DOX-treated MCF-7 human breast cancer cells. A cotreatment with DOX and a PEMF potentiated the reduction in MCF-7 cell viability compared to the treatment with DOX alone. The PEMF elevated DOX-induced G1 arrest by affecting cyclin-dependent kinase 2 phosphorylation and the expression of G1 arrest-related molecules, including p53, p21, cyclin E2, and polo like kinase 1. In addition, PEMF increased the DOX-induced upregulation of proapoptotic proteins, such as Fas and Bcl-2-associated X, and the downregulation of antiapoptotic proteins, including myeloid leukemia 1 and survivin. PEMF promoted the DOX-induced activation of caspases-8, -9, and -7 and poly (adenosine diphosphateribose) polymerase cleavage in MCF-7 cells. In conclusion, PEMF enhances the anticancer activity in DOX-treated MCF-7 breast cancer cells by increasing G1 cell cycle arrest and caspase-dependent apoptosis. These findings highlight the potential use of a PEMF as an adjuvant treatment for DOX-based chemotherapy against breast cancer.
Abstract
A pulsed electromagnetic field (PEMF) enhances the efficacy of several anticancer drugs. Doxorubicin (DOX) is an anticancer agent used to treat various malignancies, including breast cancer. This study examined whether a PEMF increases the anticancer effect of DOX on MCF-7 human breast cancer cells and elucidated the underlying mechanisms affected by PEMF stimulation in DOX-treated MCF-7 human breast cancer cells. A cotreatment with DOX and a PEMF potentiated the reduction in MCF-7 cell viability compared to the treatment with DOX alone. The PEMF elevated DOX-induced G1 arrest by affecting cyclin-dependent kinase 2 phosphorylation and the expression of G1 arrest-related molecules, including p53, p21, cyclin E2, and polo like kinase 1. In addition, PEMF increased the DOX-induced upregulation of proapoptotic proteins, such as Fas and Bcl-2-associated X, and the downregulation of antiapoptotic proteins, including myeloid leukemia 1 and survivin. PEMF promoted the DOX-induced activation of caspases-8, -9, and -7 and poly (adenosine diphosphateribose) polymerase cleavage in MCF-7 cells. In conclusion, PEMF enhances the anticancer activity in DOX-treated MCF-7 breast cancer cells by increasing G1 cell cycle arrest and caspase-dependent apoptosis. These findings highlight the potential use of a PEMF as an adjuvant treatment for DOX-based chemotherapy against breast cancer.
참고문헌 (30)
- Yang R, "Compound 968 reverses adriamycin resistance in breast cancer MCF-7ADR cells via inhibiting P-glycoprotein function independently of glutaminase", Cell Death Discov, 7, pp.204~ (2021)[DOI]
- Gillaizeau F, "Computerized advice on drug dosage to improve prescribing practice", Cochrane Database Syst Rev, 2013(11), pp.CD002894~ (2013)[DOI]
- Dolkart O, "Effects of focused continuous pulsed electromagnetic field therapy on early tendon-to-bone healing", Bone Joint Res, 10, pp.298~306 (2021)[DOI]
- Overholt TL, "Pulsed electromagnetic field therapy as a complementary alternative for chronic pelvic pain management in an interstitial cystitis", bladder pain syndrome patient, 2019, pp.2019, 5767568~
- Funk RHW, "A short review on the influence of magnetic fields on neurological diseases", Front Biosci(Schol Ed), 13, pp.181~189 (2021)[DOI]
- Vadalà M, "Mechanisms and therapeutic effectiveness of pulsed electromagnetic field therapy in oncology", Cancer Med, 5, pp.3128~3139 (2016)[DOI]
- Chen MY, "In vitro and in vivo study of the effect of osteogenic pulsed electromagnetic fields on breast and lung cancer cells", Technol Cancer Res Treat, 21, pp.15330338221124658~ (2022)[DOI]
- Sengupta S, "A review on the use of magnetic fields and ultrasound for non-invasive cancer treatment", J Adv Res, 14, pp.97~111 (2018)[DOI]
- Woo SH, "Pulsed electromagnetic field potentiates etoposide-induced MCF-7 cell death", BMB Rep, 55, pp.148~153 (2022)[DOI]
- Ramazi S, "Synergistic cytotoxic effects of an extremely low-frequency electromagnetic field with doxorubicin on MCF-7 cell line", Sci Rep, 13, pp.8844~ (2023)[DOI]
- Lüpertz R, "Dose-and time-dependent effects of doxorubicin on cytotoxicity, cell cycle and apoptotic cell death in human colon cancer cells", Toxicology, 271, pp.115~121 (2010)[DOI]
- Smirnikhina SA, "Improving homology-directed repair in genome editing experiments by influencing the cell cycle", Int J Mol Sci, 23, pp.5992~ (2022)[DOI]
- Gogolin S, "CDK4 inhibition restores G(1)-S arrest in MYCN-amplified neuroblastoma cells in the context of doxorubicin-induced DNA damage", Cell Cycle, 12, pp.1091~1104 (2013)[DOI]
- Shao Z, "p53 independent G1 arrest and apoptosis induced by adriamycin", Chin Med Sci J, 12, pp.71~75 (1997)
- Lee TK, "Doxorubicin-induced apoptosis and chemosensitivity in hepatoma cell lines", Cancer Chemother Pharmacol, 49, pp.78~86 (2002)[DOI]
- Cuadrado M, "p27Kip1 stabilization is essential for the maintenance of cell cycle arrest in response to DNA damage", Cancer Res, 69, pp.8726~8732 (2009)[DOI]
- Yaghoubi F, "A functionalized graphene oxide with improved cytocompatibility for stimuli-responsive co-delivery of curcumin and doxorubicin in cancer treatment", Sci Rep, 12, pp.1959~ (2022)[DOI]
- Waldorff EI, "Pulsed electromagnetic field applications : a corporate perspective", J Orthop Translat, 9, pp.60~68 (2017)[DOI]
- Loja T, "Influence of pulsed electromagnetic and pulsed vector magnetic potential field on the growth of tumor cells", Electromagn Biol Med, 33, pp.190~197 (2014)[DOI]
- Crocetti S, "Low intensity and frequency pulsed electromagnetic fields selectively impair breast cancer cell viability", PLoS One, 8, pp.e72944~ (2013)[DOI]
- Radeva M, "Differences in lethality between cancer cells and human lymphocytes caused by LF-electromagnetic fields", Bioelectromagnetics, 25, pp.503~507 (2004)[DOI]
- de Seze R, "Effects of 100 mT time varying magnetic fields on the growth of tumors in mice", Bioelectromagnetics, 21, pp.107~111 (2000)[DOI]
- Markov MS, "Expanding use of pulsed electromagnetic field therapies", Electromagn Biol Med, 26, pp.257~274 (2007)[DOI]
- Park JS, "Polyhexamethylene guanidine phosphate-induced ROS-mediated DNA damage caused cell cycle arrest and apoptosis in lung epithelial cells", J Toxicol Sci, 44, pp.415~424 (2019)[DOI]
- Mhone TG, "Daidzein synergizes with gefitinib to induce ROS", JNK, AKT, pp.ERK pathways to enhance lung adenocarcinoma cells chemosensitivity, Int J Biol Sci[DOI]
- Bruinsma W, "Switching Polo-like kinase-1 on and off in time and space", Trends Biochem Sci, 37, pp.534~542 (2012)[DOI]
- Giráldez S, "G1", S phase progression is regulated by PLK1 degradation through the CDK1, FASEB J, pp.2017, 31[DOI]
- Li J, "Plk1inhibition enhances the efficacy of gemcitabine in human pancreatic cancer", Cell Cycle, 15, pp.711~719 (2016)[DOI]
- Qian S, "The role of BCL-2family proteins in regulating apoptosis and cancer therapy", Front Oncol, 12, pp.985363~ (2022)[DOI]
- Liu X, "Polo-like kinase(Plk)1 depletion induces apoptosis in cancer cells", Proc Natl Acad Sci U S A, 100, pp.5789~5794 (2003)[DOI]