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Effect of Endocrine Disrupting Chemicals on Bombina orientalis Aromatase Activity Expressed in Cultured Mammalian Cells

신인철|양원석|Xiang-zi Han|Kyoung Soon Lee|Kyung-min Lee|Ji-hyun Ju
한국환경생물학회 (Korean Society Of Environmental Biology)|2011.03|환경생물 (Korean Journal of Environmental Biology)|제29권 제1호|pp. 74-80

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참고문헌

키워드

bisphenol Anonylphenolamphibianaromatase

초록

In this study we present a mammalian cell culture model that allows to study the effect of endocrine disruptors (EDCs) on aromatase activity of aquatic amphibian, Bombina orientalis. Bombina orientalis aromatase gene was subcloned into a mammalian expression vector and subsequently transfected to mammalian cells. Although the protein expression level of Bombina orientalis aromatase was low, it had a significant aromatase activity. When EDCs were added to aromatase transfected cells, aromatase activity was significantly decreased. We report here that this system may be used to monitor the effect of EDCs on aromatase activity of aquatic organisms.

Abstract

In this study we present a mammalian cell culture model that allows to study the effect of endocrine disruptors (EDCs) on aromatase activity of aquatic amphibian, Bombina orientalis. Bombina orientalis aromatase gene was subcloned into a mammalian expression vector and subsequently transfected to mammalian cells. Although the protein expression level of Bombina orientalis aromatase was low, it had a significant aromatase activity. When EDCs were added to aromatase transfected cells, aromatase activity was significantly decreased. We report here that this system may be used to monitor the effect of EDCs on aromatase activity of aquatic organisms.

참고문헌 (23)

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