Solvent Free Solid Injector (SFSI)를 이용한 수삼, 홍삼, 백삼의 향기성분 분석방법
The Analysis of Volatile Components of Fresh Ginseng, Red Ginseng and White Ginseng by Solvent Free Solid Injector (SFSI) Techniques
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초록
인삼의 휘발성 성분 분석 시 SFSI를 이용한 분석법의 최적화를 위하여 실험을 수행하였다. 최적화를 위하여 세 가지 시료조제방법인 생체시료, oven건조와 동결건조를 검토하였고, GC injector 온도는 100, 150, 200, 250, 300℃에서의 휘발성분 분석효율을 검토하였으며, 모세관에서 시료의 기화시간인 preheating time은 3, 5, 7, 10, 15 min의 조건에서 검토한 결과, 시료 조제방법은 생체시료를 그대로 사용 시, GC injector 온도는 250℃일 때 preheating time은 10 min일 때 가장 분석효율이 높았다. 휘발성분 분석을 위해 사용된 인삼은 금산에서 재배한 6년근 삼으로 수삼, 백삼, 홍삼을 사용하였으며, SFSI가 장착된 GC/MS에 의해 휘발성분을 분석, 동정하였다. 분석결과 수삼에서는 33종, 백삼에서는 36종, 홍삼에서는 38종의 휘발성분이 분석되어져 SFSI에 의한 분석법이 인삼의 휘발성분을 분석, 동정하는 방법으로 이용될 수 있음을 보여주고 있다.
Abstract
An experimental design has been used to optimize the analysis of volatile components from fresh ginseng using solvent free solid injector (SFSI). The analysis were performed at three different sample preparing methods (fresh, oven drying and freezed drying), different injector port temperatures (100, 150, 200, 250 and 300℃) and different preheating time (3, 5, 7, 10 and 15 min) according to the above experimental design. The optimum conditions from analytical results were fresh sample (sample preparing methods), 250℃ (injector temperature) and 10 min (preheating time). Ginsengs grown for six years at Gumsan were prepared for fresh, white and red ginsengs. These fresh, white and red gingsengs were analyzed for their volatile components by GC/MS equipped with SFSI according to the above the optimum conditions. A total of thirty-three volatile components were identified in fresh ginseng by SFSI, thirty-six in white ginseng and thirty-eight in red ginseng. These results suggested that the SFSI method could be used for isolating volatile components in ginsengs.
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