거미 서폐의 미세구조와 석면노출 환경의 모니터링을 위한 생물지표적 유용성 평가에 관한 연구
Microstructure of Spider Booklung as Bio-indicator for Monitoring Environmental Asbestos Exposure
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Orb-web spiders can be considered as an effective bio-indicative animal to monitor theecological air pollution of certain habitat since they have limited shifting ability during their lifespans. In this study we revealed the fine structural characteristics of booklung in the orb-web spiderNephila clavata, and its bio-indicative significance in the monitoring environmental asbestos exposure. The high resolution scanning electron microscopy results suggest that the cuticular spikesnear the atrium could be act as an effective filtering structure for dust materials. Furthermore,inner cuticular spikes which protruded across the air sacs, have the functions not only sustainingappropriate respiratory volume of each air sac but also help to enhance respiratory capacity bymaximizing the gross surface area for gas exchange. Interestingly, in spite of short exposure, theasbestose fibrils were effectively captured on the surface of the atrial cuticular spikes. Furthermore,histologic damages were observed due to penetration of asbestos fibrils through air sacs and fixedon the hemolymph space. In addition, although accumulated mass of hemocytes were observednear the fibrils, there were no asbestos bodies or coagulated hemocytes were found on the surfaceof the fibrils, This could be mainly due to the short exposure period towards asbestos. Briefly,these results indicate the spider’s booklung could be valuable tool in the detection ecological airpollutants.
Abstract
Orb-web spiders can be considered as an effective bio-indicative animal to monitor theecological air pollution of certain habitat since they have limited shifting ability during their lifespans. In this study we revealed the fine structural characteristics of booklung in the orb-web spiderNephila clavata, and its bio-indicative significance in the monitoring environmental asbestos exposure. The high resolution scanning electron microscopy results suggest that the cuticular spikesnear the atrium could be act as an effective filtering structure for dust materials. Furthermore,inner cuticular spikes which protruded across the air sacs, have the functions not only sustainingappropriate respiratory volume of each air sac but also help to enhance respiratory capacity bymaximizing the gross surface area for gas exchange. Interestingly, in spite of short exposure, theasbestose fibrils were effectively captured on the surface of the atrial cuticular spikes. Furthermore,histologic damages were observed due to penetration of asbestos fibrils through air sacs and fixedon the hemolymph space. In addition, although accumulated mass of hemocytes were observednear the fibrils, there were no asbestos bodies or coagulated hemocytes were found on the surfaceof the fibrils, This could be mainly due to the short exposure period towards asbestos. Briefly,these results indicate the spider’s booklung could be valuable tool in the detection ecological airpollutants.
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