Lead Induced Organic Acid Exudation and Citrate Enhanced Pb Uptake in Hydroponic System
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The influence of Pb-citrate complex formation on Pb uptake and the effect of Pb on organic acid exudation were investigated using four plant species, viz., sunflower (Helianthus annuus L), Indian mustard (Brassica juncea), canola (Brassica napus) and vetiver grass (Vetiveria zizanioides) under hydroponic conditions. Seedlings were exposed to different levels of Pb and Pb-citrate for 24 hrs and subsequently Pb distributions in plant shoot, root and hydroponic solution were measured. The dissolved organic carbon (DOC) concentration generally decreased as the concentration of Pb in the hydroponic solution increased. In contrast to DOC, the total organic acid concentrations exuded from Indian mustard roots significantly increased (424 to 6656 mg kg-1) with increased Pb treatment, implying that exuding organic acids were involved in Pb accumulation in Indian mustard. The complexation of Pb with citrate enhanced Pb accumulation in the above ground portions. Lead concentration in Indian mustard increased from 2.05 mg kg-1 to 6.42 mg kg-1 when the concentration of citrate in solution increased from 0 to 50 mg L-1. This result showed enhanced translocation of Pb from root to shoot with observation of transfer coefficient (Kt) increase from 2.03E-3 to 5.72E-3.
Abstract
The influence of Pb-citrate complex formation on Pb uptake and the effect of Pb on organic acid exudation were investigated using four plant species, viz., sunflower (Helianthus annuus L), Indian mustard (Brassica juncea), canola (Brassica napus) and vetiver grass (Vetiveria zizanioides) under hydroponic conditions. Seedlings were exposed to different levels of Pb and Pb-citrate for 24 hrs and subsequently Pb distributions in plant shoot, root and hydroponic solution were measured. The dissolved organic carbon (DOC) concentration generally decreased as the concentration of Pb in the hydroponic solution increased. In contrast to DOC, the total organic acid concentrations exuded from Indian mustard roots significantly increased (424 to 6656 mg kg-1) with increased Pb treatment, implying that exuding organic acids were involved in Pb accumulation in Indian mustard. The complexation of Pb with citrate enhanced Pb accumulation in the above ground portions. Lead concentration in Indian mustard increased from 2.05 mg kg-1 to 6.42 mg kg-1 when the concentration of citrate in solution increased from 0 to 50 mg L-1. This result showed enhanced translocation of Pb from root to shoot with observation of transfer coefficient (Kt) increase from 2.03E-3 to 5.72E-3.
참고문헌 (45)
- Cheng, W., "Investigating short term carbon flows in the rhizosphere of different plant species, using isotopic trapping", Agron. J., 86, pp.782~788 (1994)
- Jones, D. L., "Organic acids in the rhizosphere - a critical review", Plant and Soil., 205, pp.25~44 (1998)
- Nye, P. H., "pH changes across the rhizosphere induced by roots", Plant and Soil., 61, pp.7~26 (1981)
- Baziramakenga, R., , Soil Biol. Biochem., 27(3), pp.349~356 (1995)
- Nigam, R., "Cadmium mobilization and plant availability-the impact of organic acids commonly exuded from roots", Plant and Soil, 230, pp.107~113 (2001)
- Wu, L. H., "Effects of EDTA and low molecular weight organic acids on soil solution properties of a heavy metal polluted soil", Chemosphere (2003)
- Onyatta, J. O., "Kinetics of cadmium release from selected tropical soils from Kenya by low-molecular-weight organic acid", Soil Sci, 168(4), pp.234~252 (2003)
- Uren, N. C., "The role of root exudates in nutrient acquisition", Adv.Plant Nut, 3, pp.79~114 (1988)
- Cieśliński, G., "Low-molecular-weight organic acids in rhizosphere soils of durum wheat and their effect on cadmium bioaccumulation", Plant and Soil., 203, pp.109~117 (1998)
- McFee, W. W., "Carbon forms and functions in forest soils", pp.43~62 (1995), Soil Sci. Soc.Am.
- Strobel, B. W., "Influence of vegetation on low-molecular-weight carboxylic acids in soil solution-a review", Geoderma, 99(3), pp.169~198 (2001)
- Cieśliński, G., "Low molecular weight organic acids released from roots of durum wheat and flax into sterile nutrient solutions", J. Plant Nut., 20(6), pp.753~763 (1997)
- Alloway, B. J., "Heavy metals in soils", pp.7~27 (1990), Blackie and sons
- Salt, D. E., "Zinc ligands in the metal hyperaccumulator Thlaspi caerulescens as determined using X-ray absorption spectroscopy", Environ. Sci. Tech., 33, pp.713~717 (1999)
- Siegal, H., "Concepts in metal toxicology: metal ions in biological systems", pp.38~65 (1995), Dekker
- Zenk, M. H., "Heavy metal detoxification in higher plants-a review", Gene., 179, pp.21~30 (1996)
- Cobbett, C. S., "Phytochelatin biosynthesis and function in heavy-metal detoxification", Curr. Opin. Plant Biol., 3, pp.211~216 (2000)
- Knight, B., "Zinc and cadmium uptake by the yperaccumulator Thlaspi caerulescens in contaminated soils and its effects on the concentration and chemical speciation of metals in soil solution", Plant and Soil., 197, pp.71~78 (1997)
- Wenzel, W. W., "Rhizosphere characteristics of indigenously growing nickel hyperaccumulator and excluder plants on serpentine soil", Environ.Poll, 123(1), pp.131~138 (2003)
- Whiting, S. N., "Zinc accumulation by Thlaspi caerulescens from soils with different Zn availability: a pot study", Plant and Soil, 236, pp.11~18 (2001)
- Krishnamurti, G. S. R., "Kinetics of cadmium release from soils as influenced by organic acids:Implication in cadmium availability", J. Environ.Qual., 26, pp.271~277 (1997)
- Naidu, R., "Effect of different organic ligands on cadmium sorption by and extractability from soils", Soil Sci. Soc. Am. J., 62, pp.644~650 (1998)
- Reuter, J. H., "Importance of heavy metal-organic matter interactions in natural waters", Geochim. Cosmodhim. Acta., 41, pp.325~334 (1977)
- Kramer, U., "Free histidine as a metal chelator in plants that accumulate nickel", Nature, 379, pp.635~638 (1996)
- Lee, J., "The relation between nickel and citric acid in some nickel-accumulating plants", Phytochemistry, 17, pp.1033~1035 (1978)
- Sauvé, S., "Speciation and complexation of cadmium in extracted soil solutions", Environ.Sci. Tech., 34(2), pp.291~296 (2000)
- Vulkan, R., "Copper speciation and impacts on bacterial biosensors in the pore water of coppercontaminated soils", Environ. Sci. Tech., (24), pp.5115~5121 (2000)
- Chen, Z., "Determination of carboxylic acids from plant root exudates by ion exclusion chromatography-electrospray ionization mass spectrometry", Chromatographia, 67, pp.113~117 (2007)
- Kim, K-R., "Heavy metal distribution, bioaccessibility and phytoavailability in long-term contaminated soils from Lake Macquarie, Australia", Aust. J. Soil Res., 47(2), pp.166~176 (2009)
- Blaylock, M. J., "Enhanced accumulation of Pb in Indian mustard by soil-applied chelating agents", Environ. Sci. Tech., 31(3), pp.860~865 (1997)
- Ebbs, S. D., "Phytoextraction of Zinc by Oat (Avena sativa), Barley (Hordeum vulgare), and Indian mustard (Brassica juncea)", Environ. Sci. Tech., 32, pp.802~806 (1998)
- Ensley, B. D., "Phytoremediation of toxic metals", pp.89~106 (2000), John Wiley & Sons
- Qin, F., "Effects of 1low-molecular-weight organic acids and residence time on desorption of Cu, Cd, and Pb from soils", Chemosphere, 57(4), pp.253~263 (2004)
- Pinton, R., "The rhizosphere", pp.19~40 (2001), Marcel Dekker
- Prikryl, A., "Root exudates of plants. VI. Wheat root exudation as dependent on growth, concentration gradient of exudates and the presence of bacteria", Plant and Soil., 57, pp.69~83 (1980)
- Barber, D. A., "Microbial growth in the rhizosphere", Soil Biol. Biochem., 9, pp.305~308 (1977)
- Thurman, E.M., "Organic geochemistry of natural waters" (1985), Martinus Nijhoff
- Hees, P. A. Wv., "Determination of low molecular weight organic acids in soil solution by HPLC", Talanta., 48, pp.173~179 (1999)
- Hees, P. A. Wv., "Low molecular weight organic acids and their complexes in soil solution-composition, distribution and seasonal variation in three podzolized soils", Geoderma., 94, pp.173~200 (2000)
- Mench, M., "Metal binding with root exudates of low molecular weight", J. Soil Sci., 39, pp.521~ (1988)
- Mench, M, "Mobilization of cadmium and other metals from two soils by root exudates of Zea mays L., Nicotiana tabacum L. and Nicotiana rustica L", Plant and Soil., 132, pp.187~196 (1991)
- Collins, R. N., "Organic ligand and pH effects on isotopically exchangeable cadmium in polluted soils", Soil Sci. Soc. Am. J., 67, pp.112~121 (2003)
- Stumm, W., "Coordination interactions between soil solids and water: An aquatic chemist's point of view", Geoderma., 38, pp.19~30 (1986)
- Jones, D. L., "Role of root derived organic acids in the mobilization of nutrients from the rhizosphere", Plant and soil., 166, pp.247~257 (1994)
- White, M. C., "Metal complexation in xylem fluid. III Electrophoretic evidence", Plant physiol., 67, pp.311~315 (1981)