바이오플락 양식기술 적용 넙치, Paralichthys olivaceus 수용밀도에 따른 수질변화 및 혈액학적 성상 변화
Changes in water quality and hematological parameters according to the stocking density of olive flounder, Paralichthys olivaceus raised in bio-floc environment
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본 연구에서 바이오플락 환경에서 적정 밀도 구간 산정을 위한 실험을 진행했으며, 본 실험의 결과 밀도 구간 Group 1 (40마리 m-2)과 Group 2 (60마리 m-2)에서는 수질안정을 통해 생리적 변화 없이 13주간 실험이 이루어졌지만, 밀도 구간 Group 3 (80마리 m-2)과 Group 4 (100마리 m-2)에서는 아질산염 안정화 실패, 혈액성상 및 혈장성분의 변화를 나타내었다. 물론, 유수를 하지 않는 바이오플락 시스템 특성상 고밀도 구간에 따른 밀도 스트레스뿐만 아니라, 고밀도 구간의 수질불안정에 따른 높은 암모니아 및 아질산 농도에 따른 영향도 복합적으로 고려해야 할 것이다. 본 실험의 결과 밀도 60마리 m-2까지의 밀도 구간에서 수질안정과 함께 혈액학적 성분의 유의적 변화 없이 100 g 크기의 넙치 사육양성에 적합할 것으로 보인다.
Abstract
The Paralichthys olivaceus (mean weight 34.3±3.5 g) was used in the conduction of density experiment in this study to establish the optimal density determination for a period of 13 weeks. The density consisted of four groups. These were group 1 (500 fish, 40 fish m-2), group 2 (750 fish, 60 fish m-2), group 3 (1,000 fish, 80 fish m-2) and group 4 (1,250 fish, 100 fish m-2), each in 12.56 m2 circular water tanks. In the high density groups, such as groups 1 and 2, the nitrite was increased to over 21 mg L-1 (group 3) and 25 mg L-1 (group 4). The experiment of the groups was terminated by skin ulcers and mortality after 49 days for group 3 and 43 days for group 4. The water quality environment, such as the water temperature, dissolved oxygen, salinity and pH, remained constant. The ammonia and nitrite in groups 1 and 2 remained stable after ammonia and nitrite peak. However, the experiments in groups 3 and 4 were terminated due to the mortality induced by high nitrite. Hematological parameters, such as hemoglobin and hematocrit, were significantly decreased in the high density groups. The plasma components were significantly changed in the high density groups, such as groups 3 and 4. The mean weight of groups 1 and 2 after 13 weeks were 91.3 g and 83.7 g, respectively. The survival rates were 99.4% and 98.2%, respectively. The final FCR was 0.6 in both groups. The results of this study show that the density of 80 fish m-2 or more induce mortality due to high nitrite with hematological changes. Additionally, they indicate that the 60 fish m-2 indicate proper density in bio-floc environment in olive flounder weighing less than 100 g.
참고문헌 (28)
- 배선혜, "바이오플락 및 일반 해수에서 질산염의 넙치 (Paralichthys olivaceus)에 미치는 급성 독성 및 혈액학적 성상의 변화", 환경생물, 35(3), pp.373~379 (2017), 한국환경생물학회[DOI]
- Bakhshi F, "Use of different carbon sources for the biofloc system during the grow-out culture of common carp(Cyprinus carpio L. )fingerlings", Aquaculture, 484, pp.259~267 (2018)
- Dauda AB, "Influence of carbon", nitrogen ratios on biofloc production and biochemical composition and subsequent effects on the growth, physiological status and disease resistance of African catfish(Clarias gariepinus)cultured in glycerol-based biofloc systems, 2018, pp.483, 120~130
- Hegazi MM, "Oxidative stress and antioxidant enzymes in liver and white muscle of Nile tilapia juveniles in chronic ammonia exposure", Aquat. Toxicol, 99, pp.118~125 (2010)
- Jensen FB, "Nitrite disrupts multiple physiological functions in aquatic animals", Comp. Biochem. Physiol. A-Mol. Integr. Physiol, 135, pp.9~24 (2003)
- Kamilya D, "Biofloc technology application in indoor culture of Labeo rohita(Hamilton, 1822)fingerlings : The effects on inorganic nitrogen control, growth and immunity", Chemosphere, 182, pp.8~14 (2017)
- Kim JH, "The selenium accumulation and its effect on growth, and haematological parameters in red sea bream, Pagrus major, exposed to waterborne selenium", Ecotoxicol. Environ. Saf, 104, pp.96~102 (2014)
- Kim JH, "The lead accumulation and hematological findings in juvenile rock fish Sebastes schlegelii exposed to the dietary lead(II)concentrations", Ecotoxicol. Environ. Saf, 115, pp.33~39 (2015)
- Kim JH, "The chromium accumulation and its physiological effects in juvenile rockfish, Sebastes schlegelii, exposed to different levels of dietary chromium(Cr6+)concentrations", Environ. Toxicol. Pharmacol, 41, pp.152~158 (2016)
- 김준환, "암모니아 급성노출에 의한 바이오플락 사육 넙치(Paralichthys olivaceus)의 혈액학적 성상 및 항산화반응의 변화", 한국수산과학회지, 50(6), pp.750~755 (2017), 한국수산과학회[DOI]
- Kim JH, "Toxic effects of juvenile sablefish, Anoplopoma fimbria by ammonia exposure at different water temperature", Environ. Toxicol. Pharmacol, 54, pp.169~176 (2017)
- Kim JH, "Effects of dietary chromium exposure to rockfish, Sebastes schlegelii are ameliorated by ascorbic acid", Ecotoxicol. Environ. Saf, 139, pp.109~115 (2017)
- Kim JH, "Toxic effects on bioaccumulation and hematological parameters of juvenile rockfish Sebastes schlegelii exposed to dietary lead(Pb)and ascorbic acid", Chemosphere, 176, pp.131~140 (2017)
- Kim JH, "Effects of waterborne nitrite on hematological parameters and stress indicators in olive flounders, Paralichthys olivaceus, raised in bio-floc and seawater", Chemosphere, 209, pp.28~34 (2018)
- Kim JH, "Bio-floc technology application in flatfish Paralichthys olivaceus culture : Effects on water quality, growth, hematological parameters, and immune responses", Aquaculture, 495, pp.703~709 (2018)
- Kim JH, "Temperature-mediated changes in stress responses, acetylcholinesterase, and immune responses of juvenile olive flounder Paralichthys olivaceus in a bio-floc environment", Aquaculture, 506, pp.453~458 (2019)
- Kim JH, "Toxic effects of nitrogenous compounds(ammonia, nitrite, and nitrate)on acute toxicity and antioxidant responses of juvenile olive flounder, Paralichthys olivaceus", Environ. Toxicol. Pharmacol, 67, pp.73~78 (2019)
- Kim JH, "Changes in hematological parameters and heat shock proteins in juvenile sablefish depending on water temperature stress", J. Aquat. Anim. Health, pp.1~7 (2019)
- Kjartansson H, "Effects of different stocking densities on physiological parameters and growth of adult Atlantic salmon(Salmo salar L. )reared in circular tanks", Aquaculture, 73, pp.261~274 (1988)
- 이상민, "사육밀도에 따른 쏘가리(Siniperca scherzeri)의 성장 및 체조성 변화", 한국수산과학회지, 50(6), pp.762~769 (2017), 한국수산과학회[DOI]
- Mansour AT, "Effects of carbon sources and plant protein levels in a biofloc system on growth performance, and the immune and antioxidant status of Nile tilapia(Oreochromis niloticus)", Fish Shellfish Immunol, 64, pp.202~209 (2017)
- Martins GB, "The utilization of sodium bicarbonate, calcium carbonate or hydroxide in biofloc system : water quality, growth performance and oxidative stress of Nile tilapia(Oreochromis niloticus)", Aquaculture, 468, pp.10~17 (2017)
- Montero D, "High stocking density produces crowding altering some physiological and biochemical parameters in gilthead seabream, Sparus aurata, juveniles", Fish Physiol. Biochem, 20, pp.53~60 (1999)
- NIFS, "Standard manual of olive flounder culture", National Institute of Fisheries Science, ~ (2016)
- Rafatnezhad S, "Effects of stocking density on haematological parameters, growth and fin erosion of great sturgeon(Huso huso)juveniles", Aquac. Res, 39, pp.1506~1513 (2008)
- Wagner EJ, "Effects of rearing density upon cutthroat trout hematology, hatchery performance, fin erosion, and general health and condition", N. Am. J. Aquacult, 59, pp.173~187 (1997)
- Xu WJ, "Effects of bioflocs on growth performance, digestive enzyme activity and body composition of juvenile Litopenaeus vannamei in zero-water exchange tanks culture tanks manipulating C", N ratio in feed, 2012, pp.356-357, 147~152
- Zhao P, "The application of bioflocs technology in high-intensive, zero exchange farming systems of Marsupenaeus japonicus", Aquaculture, 354-355, pp.97~106 (2012)