유전자변형 작물이 토양 미생물상에 미치는 영향
The Effects of Genetically Modified Crops on Soil Microbial Community
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BACKGROUND: Genetically modified (GM) crops must receive relevant regulator’s authorization before they can be sold as seed or used food, feed and processing. Before approving any GM crop, the relevant government ministries are required to examine environmental risk assessment to make scientifically sound and socially acceptable decisions. But one of the least studied and understood areas in the environmental risk assessment of GM crops are their impact on soil microbial community. METHODS AND RESULTS: Recently, advanced methods have been developed to characterize the soil microbial community in various environments. In this study, the culture-dependent and culture-independent technical approaches for profiling soil microbial communities are summarized and their applicability to assess GM crops are discussed. CONCLUSION(S): We concluded that the effect of GM crops on soil microbial community need to be assessed on a case by case basis. The combination of culture-dependent and culture-independent method was necessary for reliable and detailed assessment of effect of GM crops on soil microbial community.
Abstract
BACKGROUND: Genetically modified (GM) crops must receive relevant regulator’s authorization before they can be sold as seed or used food, feed and processing. Before approving any GM crop, the relevant government ministries are required to examine environmental risk assessment to make scientifically sound and socially acceptable decisions. But one of the least studied and understood areas in the environmental risk assessment of GM crops are their impact on soil microbial community. METHODS AND RESULTS: Recently, advanced methods have been developed to characterize the soil microbial community in various environments. In this study, the culture-dependent and culture-independent technical approaches for profiling soil microbial communities are summarized and their applicability to assess GM crops are discussed. CONCLUSION(S): We concluded that the effect of GM crops on soil microbial community need to be assessed on a case by case basis. The combination of culture-dependent and culture-independent method was necessary for reliable and detailed assessment of effect of GM crops on soil microbial community.
참고문헌 (45)
- Ahrenholtz, I., "Increased killing of Bacillus subtilis on the hair roots of transgenic T4 lysozyme-producing potatoes", Appl. Environ. Microb, 66, pp.1862~1865 (2000)
- Angle, J. S., "Release of Transgenic Plants- Biodiversity and Population-Level Considerations", Mol. Ecol, 3, pp.45~50 (1994)
- Barton, J. E., "Genetically modified crops and the environment", Agron. J, 92, pp.797~803 (2000)
- Baumgarte, S., "Field studies on the environmental fate of the Cry1Ab Bt-toxin produced by transgenic maize (MON810) and its effect on bacterial communities in the maize rhizosphere", Mol. Ecol, 14, pp.2539~2551 (2005)
- Bruinsma, M., "Effects of genetically modified plants on microbial communities and processes in soil", Biol.Fert. Soils, 37, pp.329~337 (2003)
- Clausen, M., "Antifungal activity of a virally encoded gene in transgenic wheat", Nat. Biotech, 18, pp.446~449 (2000)
- Daniell, H., "Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection", Curr. Genet, 39, pp.109~116 (2001)
- De Vries, J., "The bacteriolytic activity in transgenic potatoes expressing a chimeric T4 lysozyme gene and the effect of T4 lysozyme on soil-and phytopathogenic bacteria", Syst. Appl. Microbiol, 22, pp.280~286 (1999)
- Di Giovanni, G. D., "Comparison of parental and pransgenic alfalfa rhizosphere bacterial communities using biolog GN metabolic fingerprinting and enterobacterial repetitive intergenic consensus sequence- PCR (ERIC-PCR)", Microb. Ecol, 37, pp.129~139 (1999)
- Donegan, K. K., "Changes in levels, species and DNA fingerprints of soil-microorganisms associated with cotton expressing the Bacillus thuringiensis Var. Kurstaki endotoxin", Appl. Soil. Ecol, 2, pp.111~124 (1995)
- Donegan, K. K., "Decomposition of genetically engineered tobacco under field conditions: Persistence of the proteinase inhibitor I product and effects on soil microbial respiration and protozoa, nematode and microarthropod populations", J. Appl. Ecol, 34, pp.767~777 (1997)
- Dunfield, K. E., "Diversity of bacterial communities in the rhizosphere and root interior of field-grown genetically modified Brassica napus", Fems Microbiol. Ecol, 38, pp.1~9 (2001)
- Filion, M., "Do transgenic plants affect rhizobacteria populations", Microb. Biotechnol, 1, pp.463~475 (2008)
- Germida, J. J., "Impact of genetically modified crops on soil-and plantassociated microbial communities", J. Environ. Qual, 33, pp.806~815 (2004)
- Griffiths, B. S., "Testing genetically engineered potato, producingthe lectins GNA and Con A, on non-targetsoil organisms and processes", J. Appl. Ecol, 37, pp.159~170 (2000)
- Heuer, H., "Effects of T4 lysozyme release from transgenic potato roots on bacterial rhizosphere relative to communities are negligible natural factors", Appl. Environ. Microb, 68, pp.1325~1335 (2002)
- Heuer, H., "Bacterial phyllosphere communities of Solanum tuberosum L. and T4- lysozyme-producing transgenic variants", Fems Microbiol Ecol, 28, pp.357~371 (1999)
- James, C., "Global Status of Commercialized Biotech" (2011), GM Crops
- Kirk, J. L., "Methods of studying soil microbial diversity", J Microbiol Meth, 58, pp.169~188 (2004)
- Kremer, R. J., "Glyphosate affects soybean root exudation and rhizosphere micro-organisms", Int J Environ an Ch, 85, pp.1165~1174 (2005)
- 이은희, "폐가스 처리용 바이오필터에 미생물 군집 분석 기법의 적용", Korean Chemical Engineering Research(HWAHAK KONGHAK), 48(2), pp.147~156 (2010), 한국화학공학회
- Libiakova, G., "Efficacy of an introncontaining kanamycin resistance gene as a selectable marker in plant transformation", Plant Cell Rep, 20, pp.610~615 (2001)
- Liu, B., "Effects of transgenic plants on soil microorganisms", Plant Soil, 271, pp.1~13 (2005)
- Lorenz, M. G., "Mechanism of retarded DNA degradationand prokaryotic origin of DNases in nonsterilesoils", Syst Appl Microbiol, 20, pp.513~521 (1997)
- Losey, J. E., "Transgenic pollen harms monarch larvae", Nature, 399, pp.214~214 (1999)
- Lottmann, J., "Phenotypic and genotypic characterization of antagonistic bacteria associated with roots of transgenic and non-transgenic potato plants", Microbiol Res, 156, pp.75~82 (2001)
- Lukow, T., "Use of the T-RFLP technique to assess spatial and temporal changes in the bacterial community structure within an agricultural soil planted with transgenic and non-transgenic potato plants", Fems Microbiol Ecol, 32, pp.241~247 (2000)
- Lynch, J. M., "Microbial diversity in soil: ecological theories, the contribution of molecular techniques and the impact of transgenic plants and transgenic microorganisms", Biol Fert Soils, 40, pp.363~385 (2004)
- Maizel, A., "Temporally and spatially controlled induction of gene expression in Arabidopsis thaliana", Plant J, 38, pp.164~171 (2004)
- Marschner, P., "Development of specific rhizosphere bacterial communities in relation to plant species, nutrition and soil type", Plant Soil, 261, pp.199~208 (2004)
- Neal, J. L., "Changes in the rhizosphere microflora of spring wheat induced by disomic substitution of a chromosome", Can J Microbiol, 16, pp.153~158 (1970)
- Nielsen, K. M., "Transformation of Acinetobacter sp strain BD413 (pFG4 Delta nptII) with transgenic plant DNA in soil microcosms and effects of kanamycin on selection of transformants", Appl Environ Microb, 66, pp.1237~1242 (2000)
- Nocker, A., "Genotypic microbial community profiling: A critical technical review", Microbial Ecology, 54, pp.276~289 (2007)
- Oger, P., "Genetically engineered plants producing opines alter their biological environment", Nat Biotechnol, 15, pp.369~372 (1997)
- Paget, E., "The fate of recombinant plant DNA in soil", Eur J Soil Biol, 34, pp.81~88 (1998)
- Rodenburg, K. W., "Single-Stranded-DNA Used as an Efficient New Vehicle for Transformation of Plant-Protoplasts", Plant Mol Biol, 13, pp.711~719 (1989)
- Saxena, D., "Transgenic plants-Insecticidal toxin in root exudates from Bt corn", Nature, 402, pp.480~480 (1999)
- Saxena, D., "Insecticidal toxin from Bacillus thuringiensis is released from roots of transgenic Bt corn in vitro and in situ", Fems Microbiol Ecol, 33, pp.35~39 (2000)
- Schmalenberger, A., "Bacterial community composition in the rhizosphere of a transgenic, herbicide-resistant maize (Zea mays ) and comparison to its non-transgenic cultivar Bosphore", Fems Microbiol Ecol, 40, pp.29~37 (2002)
- Siciliano, S. D., "Taxonomic diversity of bacteria associated with the roots of field-grown transgenic Brassica napus cv. Quest, compared to the non-transgenic B. napus cv. Excel and B. rapa cv", Parkland, Fems Microbiol Ecol, 29, pp.263~272 (1999)
- Siciliano, S. D., "Differences in the microbial communities associated with the roots of different cultivars of canola and wheat", Can J Microbiol, 44, pp.844~851 (1998)
- Sims, S. R., "Soil inactivation of the Bacillus thuringiensis subsp kurstak i CryIIA insecticidal protein within transgenic cotton tissue: Laboratory microcosm and field studies", J Agr Food Chem, 45, pp.1502~1505 (1997)
- Smalla, K., "Monitoring field releases of genetically modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer", Fems Microbiol Ecol, 28, pp.261~272 (1999)
- Tilman, D., "Biodiversity and stability in grasslands", Nature, 367, pp.363~365 (1994)
- Widmer, F., "Quantification of transgenic plant marker gene persistence in the field", Mol Ecol, 6, pp.1~7 (1997)
