Enzyme Profiles of Alga-Lytic Bacterial Strain Ak-13 Related with Elimination of Cyanobacterium Anabaena Cylindrica
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Abstract
- To investigate bacteria with alga -lytic activities against Anabaenacylindrica when water blooming occurs and to study enzyme profiles associated withalga -lytic activity, various bacterial strains were isolated from surface waters andsediments in eutrophic lakes or reservoirs in Korea. Among 178 isolates, only nineisolates exhibited lytic abilities against A. cylindrica on the agar plates, and then theisolate AK-13 was selected as the strongest in lysing the cyanobacterium A. cylindrica .The strain AK -13 was characterized and identified as Sinorhizobium sp. based on fattyacid methyl ether profiles and 16S rDNA sequence. According to the results of theenzyme assays, in the strain AK-13 of Sinorhizobium sp., alginase, amylase, proteinase(caseinase and gelatinase), carboxymethyl -cellulase (CMCase), laminarinase, andlipase was produced, namely CMCase, laminarinase and protease were highly active.None of glycosidase was produced. Therefore, enzyme systems of Sinorhizobium sp.AK-13 were very complex to degrade cell walls of A. cylindrica . The peptidoglycans ofA. cylindrica may be hydrolyzed and metabolized to a range of easily utilizablemonosaccharides or other low molecular weight organic substances by Sinorhizobiumsp. AK-13.
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