The Selective Inhibitory Activity of a Fusaricidin Derivative on a Bloom-Forming Cyanobacterium, Microcystis sp
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Fusaricidin analogs, produced by Paenibacillus polymyxa, were tested for selective control of a major bloom-forming cyanobacterium, Microcystis sp. Fusaricidin (A and B mixtures) and four analogs were isolated from P. polymyxa E681 and investigated for their inhibition of cyanobacterial cell growth. Among the four fusaricidin analogs, fraction 915 Da (designated as Fus901) showed growth inhibition activity for Microcystis aeruginosa but not for Anabaena variabilis and Scenedesmus acutus. Microcystin concentration decreased up to 70% and its content per cell also decreased over 50% after 3 days. Fusaricidin exhibited growth inhibition against Gram-positive bacteria but Fus901 did not. Molecular weights of fusaricidin A and B were 883 Da and 897 Da, whereas that of Fus901 was 915 Da. Structure analysis by a ringopening method revealed a linear form for Fus901. Expression of the pod gene related to oxidative stress was increased 2.1-fold by Fus901 and that of mcyD decreased up to 40%. These results indicate that Fus901 exerts oxidative stress against M. aeruginosa. Thus, Fus901 can be used as a selective cyanobactericide without disturbing the ecological system and could help in decreasing the microcystin concentration.
Abstract
Fusaricidin analogs, produced by Paenibacillus polymyxa, were tested for selective control of a major bloom-forming cyanobacterium, Microcystis sp. Fusaricidin (A and B mixtures) and four analogs were isolated from P. polymyxa E681 and investigated for their inhibition of cyanobacterial cell growth. Among the four fusaricidin analogs, fraction 915 Da (designated as Fus901) showed growth inhibition activity for Microcystis aeruginosa but not for Anabaena variabilis and Scenedesmus acutus. Microcystin concentration decreased up to 70% and its content per cell also decreased over 50% after 3 days. Fusaricidin exhibited growth inhibition against Gram-positive bacteria but Fus901 did not. Molecular weights of fusaricidin A and B were 883 Da and 897 Da, whereas that of Fus901 was 915 Da. Structure analysis by a ringopening method revealed a linear form for Fus901. Expression of the pod gene related to oxidative stress was increased 2.1-fold by Fus901 and that of mcyD decreased up to 40%. These results indicate that Fus901 exerts oxidative stress against M. aeruginosa. Thus, Fus901 can be used as a selective cyanobactericide without disturbing the ecological system and could help in decreasing the microcystin concentration.
참고문헌 (28)
- Dittmann E, "Insertional mutagenesis of a peptide synthetase gene that is responsible for hepatotoxin production in the cyanobacterium Microcystis aeruginosa PCC 7806", Mol. Microbiol, 26, pp.779~787 (1997)
- Tillett D, "Structural organization of microcystin biosynthesis in Microcystis aeruginosa PCC7806 : an integrated peptide-polyketide synthetase system", Chem. Biol, 7, pp.753~764 (2000)
- Kaebernick M, "Light and the transcriptional response of the microcystin biosynthesis gene cluster", Appl. Environ. Microbiol, 66, pp.3387~3392 (2000)
- Baker JA, "Monitoring changing toxigenicity of a cyanobacterial bloom by molecular methods", Appl. Environ. Microbiol, 68, pp.6070~6076 (2002)
- Lawton LA, "Removal of cyanobacterial toxins(microcystins)and cyanobacterial cells from drinking water using domestic water filters", Water Res, 32, pp.633~638 (1998)
- Zhang S, "Control of fusarium wilt disease of cucumber plants with the application of a bioorganic fertilizer", Biol. Fert. Soils, 44, pp.1073~1080 (2008)
- Raza W, "Paenibacillus polymyxa : antibiotics, hydrolytic enzymes and hazard assessment", J. Plant Pathol, 90, pp.419~430 (2008)
- Kuroda J, "LI-F antibiotics, a family of antifungal cyclic depsipeptides produced by Bacillus polymyxa L-1129", Heterocycles, 53, pp.1533~1549 (2000)
- Choi SK, "Identification and functional analysis of the fusaricidin biosynthetic gene of Paenibacillus polymyxa E681", Biochem. Biophys. Res. Comm, 365, pp.89~95 (2008)
- Wood LW, "Chloroform-methanol extraction of chlorophyll a", Can. J. Fish. Aquat. Sci, 42, pp.38~43 (1985)
- Ward CJ, "Colorimetric protein phosphatase inhibition assay of laboratory strains and natural blooms of cyanobacteria: comparisons with highperformance liquid chromatographic analysis for microcystins", FEMS Microbiol. Lett, 153, pp.465~473 (1997)
- Chini V, "Absolute and relative real-time PCR in the quantification of tst gene expression among methicillin-resistant Staphylococcus aureus : evaluation by two mathematical models", Lett. Appl. Microbiol, 45, pp.479~484 (2007)
- Rinta-Kanto, "Quantification of toxin Microcystis spp, during 2003 and 2004 blooms in Wastern Lake Eric using quantitative real-time PCR", Environ. Sci. Technol, 39, pp.4198~4205 (2005)
- Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔC T method", Methods, 25, pp.402~408 (2001)
- Choi SK, "Identification of a Polymyxin synthetase gene cluster of Paenibacillus polymyxa and heterologous expression of the gene in Bacillus subtilis", J. Bacteriol, 191, pp.3350~3358 (2009)
- Lee YJ, "Field experiments on mitigation of harmful algal blooms using a sophorolipid-yellow clay mixture and effects on marine plankton", Harmful Algae, 7, pp.154~162 (2008)
- Pan G, "Removal of cyanobacterial blooms in Taihu lake using local soils, I. Equilibrium and kinetic screening on the flocculation of Microcystis aeruginosa using commercially available clays and minerals", Environ. Pollut, 141, pp.195~200 (2006)
- Han FX, "Accumulation, distribution, and toxicity of copper in sediments of catfish ponds receiving periodic copper sulfate applications", J. Environ. Qual, 30, pp.912~919 (2001)
- Drábková M, "Photodynamic therapy against cyanobacteria", Environ. Toxicol, 22, pp.112~115 (2007)
- Jeong JH, "Algicidal activity of the seaweed Corallina pilulifera against red tide microalgae", J. Appl. Phycol, 12, pp.37~43 (2000)
- Manage PM, "Algicidal effect of the bacterium Alcaligenes denitrificans on Microcystis spp", Aquat. Microb. Ecol, 22, pp.111~117 (2000)
- Kang YK, "Isolation, identification and characterization of algicidal bacteria against Stephanodiscus hantzschii and Peridinium bipes for the control of freshwater winter algal blooms", J. Appl. Phycol, 20, pp.375~386 (2008)
- Yoshida T, "Isolation and characterization of a cyanophage infecting the toxic cyanobacterium Microcystis aeruginosa", Appl. Environ. Microbiol, 72, pp.1239~1247 (2006)
- Mayali X, "Microbial community interactions and population dynamics of an algicidal bacterium active against Karenia brevis(Dinophyceae)", Harmful Algae, 1, pp.277~293 (2002)
- Nakai S, "Myriophyllum spicatum-released allelopathic polyphenols inhibiting growth of blue-green algae Microcystis aeruginosa", Water Res, 34, pp.3026~3032 (2000)
- Kajimura Y, "Fusaricidins B, C, and D, new depsipeptide antibiotics produced by Bacillus polymyxa KT-8 : isolation, structure elucidation and biological activity", J. Antibiot, 50, pp.220~228 (1997)
- Urbach E, "Multiple evolutionary origins of prochlorophytes within the cyanobacterial radiation", Nature, 335, pp.267~270 (1992)
- Nübel U, "PCR primers to amplify 16S rRNA genes from cyanobacteria", Appl. Environ. Microbiol, 63, pp.3327~3332 (1997)